CAJ | 학술논문

目的观察胃转流术(GBP)对2型糖尿病大鼠胰高血糖素样肽1(GLP-1)及小肠黏膜的影响,探讨GBP术降糖机制.方法健康雄性SD大鼠随机分为正常手术组(NO组)、正常对照组(NC组)、糖尿病手术组(DO组)、糖尿病对照组(DC组).术前及术后第1、2、4、8周分别检测各组GLP-1、空腹血糖(FPG)、胰岛素.术后2个月苏木素-伊红(HE)染色观察末段回肠黏膜病理变化,测量肠黏膜厚度和绒毛高度.结果 DO、NO组GBP术后第8周空腹GLP-1分别由术前的(7.38±1.71) pmol/L和(7.23±1.59) pmol/L升高到(17.80±1.39) pmol/L和(15.48±1.21) pmol/L(P＜0.05).DO组术后第8周FPG由术前的(17.71 ±1.99) mmol/L下降到(5.95 ±0.51) mmol/L (P＜0.05).术后各时相点DO组GLP-1、FPG明显低于相应时间点DC组(P＜0.05),NO组手术前后FPG无明显变化.DO组术后第8周胰岛素由术前的(11.93±0.75) mmol/L升高到(14.80±0.78) mmol/L(P＜0.05).DO、NO组肠黏膜厚度和绒毛高度均显著高于DC组和NC组(P＜0.05).结论 GBP术后肠黏膜的增生导致GLP-1升高可能为降糖机制之一.
목적 관찰위전류술(GBP)대2형당뇨병대서이고혈당소양태1(GLP-1)급소장점막적영향,탐토GBP술강당궤제.방법 건강웅성SD대서수궤분위정상수술조(NO조)、정상대조조(NC조)、당뇨병수술조(DO조)、당뇨병대조조(DC조).술전급술후제1、2、4、8주분별검측각조GLP-1、공복혈당(FPG)、이도소.술후2개월소목소-이홍(HE)염색관찰말단회장점막병리변화,측량장점막후도화융모고도.결과 DO、NO조GBP술후제8주공복GLP-1분별유술전적(7.38±1.71) pmol/L화(7.23±1.59) pmol/L승고도(17.80±1.39) pmol/L화(15.48±1.21) pmol/L(P＜0.05).DO조술후제8주FPG유술전적(17.71 ±1.99) mmol/L하강도(5.95 ±0.51) mmol/L (P＜0.05).술후각시상점DO조GLP-1、FPG명현저우상응시간점DC조(P＜0.05),NO조수술전후FPG무명현변화.DO조술후제8주이도소유술전적(11.93±0.75) mmol/L승고도(14.80±0.78) mmol/L(P＜0.05).DO、NO조장점막후도화융모고도균현저고우DC조화NC조(P＜0.05).결론 GBP술후장점막적증생도치GLP-1승고가능위강당궤제지일.
Objective To observe the and influence on intestinal mucosa on type 2 diabetes rats and to research the function of glucagon-like peptide-1 (GLP-1).Methods Healthy male SD rats were randomly divided into normal operation group (NO),normal control group (NC),diabetic operation group (DO) and diabetic control group (DC).The GLP-1,plasma glucose and insulin were tested preoperatively and 1,2,4,8 weeks postoperatively.Intestinal mucosa were examined by hematoxylin and eosin (HE) stain 2 months after operation.The mucosal thickness and villus height were measured by histological analysis.Results The DO group' s and NO group' s GLP-1 increased from (7.38 ± 1.71) pmol/L and (7.23 ± 1.59) pmol/L to (17.80 ± 1.39) pmol/L and (15.48 ± 1.21) pmol/L (P ＜ 0.05) at 8th week after gastric bypass operation respectively.The GLP-1 level of DO group was higher than that of DC and NC groups at corresponding time (P ＜ 0.05).The DO group' s fasting plasma glucose level was decreased which from (17.71 ± 1.99) mmol/L (preoperatively) to (5.95 ± 0.51) mmol/L (8 weeks postoperative) (P ＜ 0.05).The plasma glucose of DC group was higher than DO group at corresponding time (P ＜0.05).The DO group' s fasting plasma insulin increased from the preoperative (11.93 ± 0.75) mmol/L to (14.80 ± 0.78) mmol/L (P ＜ 0.05)at 8th week after gastric bypass operation respectively.Postoperative pathologic exam showed that the mucosal thickness and villus height in DO group and NO group were significantly higher than DC group and NC group.Conclusion The glucagons-like peptide-1 plays an important role on hypoglycemic effect of diabetic type 2 rats treated by gastric bypass operation.