中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
10期
2182-2184
,共3页
张宏伟%罗乾坤%秦涛%刘传江%胡明星%王玉柱
張宏偉%囉乾坤%秦濤%劉傳江%鬍明星%王玉柱
장굉위%라건곤%진도%류전강%호명성%왕옥주
慢性胰腺炎%微小RNA
慢性胰腺炎%微小RNA
만성이선염%미소RNA
Chronic pancreatitis%MicroRNA
目的 比较大鼠慢性胰腺炎(CP)胰腺组织与正常胰腺组织微小RNA (miRNA)表达谱的差异,探讨CP发病中潜在的关键调节作用的miRNA及其靶基因功能.方法 将16只SD大鼠随机分为实验组和对照组,分别尾部静脉注射二氯二丁基酯和无菌生理盐水建立模型,8周后检测血清淀粉酶及胰腺组织病理变化.检测分析胰腺组织miRNA表达谱差异,对差异表达的miRNA进行靶基因预测、Gene Ontology(GO)分析和信号通路分析.结果 实验组与对照组血清淀粉酶、病理组织学评分差异有统计学意义(P＜0.05);两组miRNA表达谱差异明显,与对照组胰腺组织比较,实验组胰腺组织中上调miRNA共49个,下调miRNA共37个(变化倍数＞2.0,P＜0.05),上调的部分miRNA参与胰腺组织的纤维化和细胞的凋亡.在差异表达miRNA中有24个(占27.91％)可以找到相应的靶基因,GO分析结果表明靶基因在细胞物质代谢、增殖周期、信号传导、胞外结构活动、细胞器合成等过程中起重要调节作用;信号通路分析表明基因在代谢通路、丝裂原活化蛋白激酶(MAPK)信号通路、癌症通路和Hedgehog信号通路等富集有显著变化.结论 慢性胰腺炎大鼠胰腺组织与正常大鼠胰腺组织中miRNA表达谱差异有统计学意义,miRNA通过调节靶基因的表达参与调控代谢通路、MAPK信号通路、癌症通路和Hedgehog信号通路等,在胰腺实质纤维化的进展和癌变中起重要调节作用.
目的 比較大鼠慢性胰腺炎(CP)胰腺組織與正常胰腺組織微小RNA (miRNA)錶達譜的差異,探討CP髮病中潛在的關鍵調節作用的miRNA及其靶基因功能.方法 將16隻SD大鼠隨機分為實驗組和對照組,分彆尾部靜脈註射二氯二丁基酯和無菌生理鹽水建立模型,8週後檢測血清澱粉酶及胰腺組織病理變化.檢測分析胰腺組織miRNA錶達譜差異,對差異錶達的miRNA進行靶基因預測、Gene Ontology(GO)分析和信號通路分析.結果 實驗組與對照組血清澱粉酶、病理組織學評分差異有統計學意義(P＜0.05);兩組miRNA錶達譜差異明顯,與對照組胰腺組織比較,實驗組胰腺組織中上調miRNA共49箇,下調miRNA共37箇(變化倍數＞2.0,P＜0.05),上調的部分miRNA參與胰腺組織的纖維化和細胞的凋亡.在差異錶達miRNA中有24箇(佔27.91％)可以找到相應的靶基因,GO分析結果錶明靶基因在細胞物質代謝、增殖週期、信號傳導、胞外結構活動、細胞器閤成等過程中起重要調節作用;信號通路分析錶明基因在代謝通路、絲裂原活化蛋白激酶(MAPK)信號通路、癌癥通路和Hedgehog信號通路等富集有顯著變化.結論 慢性胰腺炎大鼠胰腺組織與正常大鼠胰腺組織中miRNA錶達譜差異有統計學意義,miRNA通過調節靶基因的錶達參與調控代謝通路、MAPK信號通路、癌癥通路和Hedgehog信號通路等,在胰腺實質纖維化的進展和癌變中起重要調節作用.
목적 비교대서만성이선염(CP)이선조직여정상이선조직미소RNA (miRNA)표체보적차이,탐토CP발병중잠재적관건조절작용적miRNA급기파기인공능.방법 장16지SD대서수궤분위실험조화대조조,분별미부정맥주사이록이정기지화무균생리염수건립모형,8주후검측혈청정분매급이선조직병리변화.검측분석이선조직miRNA표체보차이,대차이표체적miRNA진행파기인예측、Gene Ontology(GO)분석화신호통로분석.결과 실험조여대조조혈청정분매、병리조직학평분차이유통계학의의(P＜0.05);량조miRNA표체보차이명현,여대조조이선조직비교,실험조이선조직중상조miRNA공49개,하조miRNA공37개(변화배수＞2.0,P＜0.05),상조적부분miRNA삼여이선조직적섬유화화세포적조망.재차이표체miRNA중유24개(점27.91％)가이조도상응적파기인,GO분석결과표명파기인재세포물질대사、증식주기、신호전도、포외결구활동、세포기합성등과정중기중요조절작용;신호통로분석표명기인재대사통로、사렬원활화단백격매(MAPK)신호통로、암증통로화Hedgehog신호통로등부집유현저변화.결론 만성이선염대서이선조직여정상대서이선조직중miRNA표체보차이유통계학의의,miRNA통과조절파기인적표체삼여조공대사통로、MAPK신호통로、암증통로화Hedgehog신호통로등,재이선실질섬유화적진전화암변중기중요조절작용.
Objective By comparing differences of microRNA (miRNA) expression profiles in chronic pancreatitis (CP) pancreatic tissue and normal pancreatic tissue of rats,to study the regulation function and protein-coding of miRNA in the fibrosis of CP organization and the biological function of the target gene.Methods Sixteen SD rats were divided into experimental and control groups randomly.Dichlorobis-butyl ester was injected via the tail vein in experimental group,and normal saline was given in control group.Serum amylase and pathological changes of pancreatic tissues after 8 weeks were observed.The differentially expressed miRNA profiles between CP and normal pancreatic tissues were analyzed by microarrays.The target gene was predicted,and the biological function (gene ontology,pathway analysis) of the target gene was investigated.Results The results showed that there was significant difference in serum amylase and histological score,and the expression levels of miRNA between two groups.As compared with control group,49 miRNAs were up-regulated,and 37 miRNAs were down-regulated (fold change ＞ 2.0,P ＜ 0.05) in experimental group.The up-regulated miRNAs were significantly related to the fibrosis and apoptosis of pancreatic tissues.Twenty-four miRNAs had their target gene.GO analysis showed that target genes played an important role in biological regulatory function.Pathway analysis revealed the pathways in mitogen-activated protein kinase (MAPK),and carcinoma signaling pathways etc.significantly changed in the CP tissues as compared with normal pancreatic tissues (P ＜ 0.05).Conclusion It was suggested miRNA expression profiles showed significant difference between CP and normal pancreatic tissues.MiRNA regulated MAPK,Hedgehog and carcinoma signaling pathways by regulating the expression of target genes,and played an important regulatory role in the fibrosis progress and canceration.
