中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
10期
2191-2193
,共3页
孙国柱%杨建凯%李胜超%赵宗茂%马波涛%朱小辉
孫國柱%楊建凱%李勝超%趙宗茂%馬波濤%硃小輝
손국주%양건개%리성초%조종무%마파도%주소휘
灯盏花素%脑损伤%液压冲击%水通道蛋白4%脑水肿
燈盞花素%腦損傷%液壓遲擊%水通道蛋白4%腦水腫
등잔화소%뇌손상%액압충격%수통도단백4%뇌수종
Breviscapine%Brain injury%Fluid percussion%Aquaporin 4%Cerebral edema
目的 观察灯盏花素对大鼠液压冲击脑损伤神经保护作用并探讨其机制.方法 成年雄性SD大鼠80只制作颅脑损伤模型,随机分为两组:模型组和干预组.干预组应用灯盏花素90 mg/kg腹腔注射.两组大鼠均于术后6、12、24 h、3、7d进行神经功能评分和组织学观察,并用干湿重法、免疫组织化学法和Western blot分别测定水肿脑组织含水量及其水通道蛋白4(AQP4)的表达变化.结果 与模型组比较,干预组大鼠神经损伤明显缓解,脑组织水肿程度减轻,以24 h、3d减轻最为明显[24 h:(82.54±1.89)％比(80.84±1.08)％;3d:(80.61±0.49)％比(79.11 ±1.14)％];神经功能评分明显改善,以24 h、3、7d3个时间点最为显著(24 h:5.83 ±0.41比7.33 ±0.82;3d:6.17 ±0.75比7.50 ±0.55;7 d:7.00±0.63比8.33 ±0.52),同时AQP4蛋白表达也出现不同程度减少,尤以24 h、3、7d显著(24 h:0.83 ±0.03比0.57 ±0.04;3 d:0.91 ±0.02比0.61 ±0.02;7d:0.64±0.05比0.42±0.07).结论 灯盏花素能够改善神经细胞受损程度,通过下调AQP4表达减轻液压冲击脑损伤脑水肿发挥神经保护作用.
目的 觀察燈盞花素對大鼠液壓遲擊腦損傷神經保護作用併探討其機製.方法 成年雄性SD大鼠80隻製作顱腦損傷模型,隨機分為兩組:模型組和榦預組.榦預組應用燈盞花素90 mg/kg腹腔註射.兩組大鼠均于術後6、12、24 h、3、7d進行神經功能評分和組織學觀察,併用榦濕重法、免疫組織化學法和Western blot分彆測定水腫腦組織含水量及其水通道蛋白4(AQP4)的錶達變化.結果 與模型組比較,榦預組大鼠神經損傷明顯緩解,腦組織水腫程度減輕,以24 h、3d減輕最為明顯[24 h:(82.54±1.89)％比(80.84±1.08)％;3d:(80.61±0.49)％比(79.11 ±1.14)％];神經功能評分明顯改善,以24 h、3、7d3箇時間點最為顯著(24 h:5.83 ±0.41比7.33 ±0.82;3d:6.17 ±0.75比7.50 ±0.55;7 d:7.00±0.63比8.33 ±0.52),同時AQP4蛋白錶達也齣現不同程度減少,尤以24 h、3、7d顯著(24 h:0.83 ±0.03比0.57 ±0.04;3 d:0.91 ±0.02比0.61 ±0.02;7d:0.64±0.05比0.42±0.07).結論 燈盞花素能夠改善神經細胞受損程度,通過下調AQP4錶達減輕液壓遲擊腦損傷腦水腫髮揮神經保護作用.
목적 관찰등잔화소대대서액압충격뇌손상신경보호작용병탐토기궤제.방법 성년웅성SD대서80지제작로뇌손상모형,수궤분위량조:모형조화간예조.간예조응용등잔화소90 mg/kg복강주사.량조대서균우술후6、12、24 h、3、7d진행신경공능평분화조직학관찰,병용간습중법、면역조직화학법화Western blot분별측정수종뇌조직함수량급기수통도단백4(AQP4)적표체변화.결과 여모형조비교,간예조대서신경손상명현완해,뇌조직수종정도감경,이24 h、3d감경최위명현[24 h:(82.54±1.89)％비(80.84±1.08)％;3d:(80.61±0.49)％비(79.11 ±1.14)％];신경공능평분명현개선,이24 h、3、7d3개시간점최위현저(24 h:5.83 ±0.41비7.33 ±0.82;3d:6.17 ±0.75비7.50 ±0.55;7 d:7.00±0.63비8.33 ±0.52),동시AQP4단백표체야출현불동정도감소,우이24 h、3、7d현저(24 h:0.83 ±0.03비0.57 ±0.04;3 d:0.91 ±0.02비0.61 ±0.02;7d:0.64±0.05비0.42±0.07).결론 등잔화소능구개선신경세포수손정도,통과하조AQP4표체감경액압충격뇌손상뇌수종발휘신경보호작용.
Objective To investigate the effect of breviscapine on neuroprotection of rats following fluid percussion injury.Methods Eighty rats were employed to establish experimental models according to Dixon' s method and randomly divided into two group:model group and intervention group.The rats in intervention group were adminstered breviscapine (90 mg/kg) by intraperitoneal injection.Nervous function score,brain water content,histological changes,and aquaporin 4 (AQP4) expression were observed by Shapira and Wahld method,dry-wet measurement,light microscopy,immunohistochemistry and Western blotting at 6,12,24 h and 3,7 d after strike respectively.Results As compared with model group,the pathological changes were significantly alleviated,and water content of edemamtous brain was significantly reduced in intervention group,especially at 24 h and 3 d after strike [24 h:(82.54 ± 1.89) ％ vs.(80.84±1.08)％; 3 d:(80.61 ±0.49)％ vs.(79.11 ±1.14)％].Nervous function score was increased significantly,especially at 24 h,3 d and 7 d after strike (24 h:5.83 ±0.41 vs.7.33 ±0.82;3 d:6.17 ±0.75 vs.7.50 ±0.55; 7 d:7.00 ±0.63 vs.8.33 ±0.52).AQP4 protein expression was decreased signifacantly at 24 h,3 d and 7 d after strock (24 h:0.83 ± 0.03 vs.0.57 ± 0.04; 3 d:0.91 ± 0.02 vs.0.61 ±0.02; 7 d:0.64 ±0.05 vs.0.42±0.07).Conclusion Breviscapine plays arole of neuroprotective effect on brain damage from fluid percussion injury by downregulating AQP4 expression and improving nervous cell injury.
