CAJ | 학술논문

目的探讨吡柔比星(THP)对人膀胱癌T24细胞凋亡的影响及其作用机制.方法使用不同浓度(0、0.1、1.0、10.0mg/L)THP作用膀胱癌T24细胞,逆转录-聚合酶链反应(RT-PCR)和Western blot检测细胞中生存素(Survivin)和半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3 mRNA及蛋白的表达;细胞计数试剂盒(CCK-8)检测细胞的增殖能力;流式细胞术检测细胞的周期及凋亡.结果与空白组比较,各THP作用组T24细胞中Survivin mRNA及蛋白水平表达降低,Caspase-3 mRNA及蛋白水平表达增高(P＜0.01);THP可抑制T24细胞的增殖,抑制率分别为(13.74±1.25)％、(36.28±0.75)％、(47.13±1.62)％,并诱导其凋亡,凋亡率为(15.29±0.98)％、(19.56±1.45)％、(23.81±0.81)％,并呈现一定的浓度和时间依赖性(P＜0.05).结论 THP可促进膀胱癌T24细胞的凋亡,其可能通过抑制细胞中Survivin的表达,增强Caspase-3的活性而发挥作用.
목적 탐토필유비성(THP)대인방광암T24세포조망적영향급기작용궤제.방법 사용불동농도(0、0.1、1.0、10.0mg/L)THP작용방광암T24세포,역전록-취합매련반응(RT-PCR)화Western blot검측세포중생존소(Survivin)화반광안선천동안산특이성단백매(Caspase)-3 mRNA급단백적표체;세포계수시제합(CCK-8)검측세포적증식능력;류식세포술검측세포적주기급조망.결과 여공백조비교,각THP작용조T24세포중Survivin mRNA급단백수평표체강저,Caspase-3 mRNA급단백수평표체증고(P＜0.01);THP가억제T24세포적증식,억제솔분별위(13.74±1.25)％、(36.28±0.75)％、(47.13±1.62)％,병유도기조망,조망솔위(15.29±0.98)％、(19.56±1.45)％、(23.81±0.81)％,병정현일정적농도화시간의뢰성(P＜0.05).결론 THP가촉진방광암T24세포적조망,기가능통과억제세포중Survivin적표체,증강Caspase-3적활성이발휘작용.
Objective To investigate the pirarubicin (THP)-induced apoptosis of hunan bladder cancer T24 cells and its mechanisms.Methods T24 cells were cultured in vitro,and incubated with different concentrations of THP (0,0.1,1.0,and 10.0 mg/L).The expression of Survivin and Cysteinyl aspartate-specific protease-3 (Caspase-3) was detected by reverse transcription-polymerase chain reaction (RT-PCR),and conformed by Western blotting.The cell proliferation was measured by growth curve.The cell cycle and apoptosis rate of T24 cells were tested by flow cytometry.Results As compared with the control group,the expression of Survivin mRNA and protein was decreased,and that of Caspase-3 mRNA and protein increased in the THP-treated group (P ＜ 0.01).The inhibition rate was (13.74 ± 1.25) ％,(36.28 ±0.75)％ and (47.13±1.62)％,and the apoptosis rate was (15.29 ±0.98)％,(19.56± 1.45) ％ and (23.81 ± 0.81) in THP 0.1,1.0 and 10.0 mg/L groups respectively.THP inhibited the proliferation of T24 cells in a time-and concentration-dependent manner (P ＜ 0.05).Conclusion THP could inhibit the growth and induce apoptosis of T24 cells,which may be principally associated with the down-regulation of Survivin and up-regulation of Caspase-3.