CAJ | 학술논문

目的探讨转录因子Twist、缺氧诱导因子(HIF)-1α在乳腺浸润性导管癌组织中的表达及其与上皮-间充质转化的关系.方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法检测90例乳腺浸润性导管癌组织及其对应癌旁组织标本中Twist、HIF-1α、E-钙黏蛋白(E-cadherin)及波形蛋白(Vimentin)的表达.结果在乳腺癌组织中Twist、HIF-1α、E-cadherin和Vimentin的阳性率分别为73.3％、68.9％、15.6％和64.4％,癌旁组织中的阳性率分别为7.8％、8.9％、75.6％和34.4％.该4种蛋白在肿瘤组织中的表达率与癌旁组织之间差异均有统计学意义(P＜0.05).Twist与HIF-1α的表达均与组织学分级和腋窝淋巴结转移有关(P＜0.05).Twist与HIF-1α的表达呈正相关(P＜0.05).Twist、HIF-1α的表达也均与E-cadherin的低表达和Vimentin的高表达密切相关(P＜0.05).结论 Twist、HIF-1α和Vimentin在乳腺癌组织中呈高表达,而E-cadherin呈低表达.HIF-1α可能通过上调Twist表达,促进上皮-间充质转化发生,成为乳腺癌浸润转移的途径之一.
목적 탐토전록인자Twist、결양유도인자(HIF)-1α재유선침윤성도관암조직중적표체급기여상피-간충질전화적관계.방법 채용면역조직화학련매균항생물소단백-과양화물매(SP)법검측90례유선침윤성도관암조직급기대응암방조직표본중Twist、HIF-1α、E-개점단백(E-cadherin)급파형단백(Vimentin)적표체.결과 재유선암조직중Twist、HIF-1α、E-cadherin화Vimentin적양성솔분별위73.3％、68.9％、15.6％화64.4％,암방조직중적양성솔분별위7.8％、8.9％、75.6％화34.4％.해4충단백재종류조직중적표체솔여암방조직지간차이균유통계학의의(P＜0.05).Twist여HIF-1α적표체균여조직학분급화액와림파결전이유관(P＜0.05).Twist여HIF-1α적표체정정상관(P＜0.05).Twist、HIF-1α적표체야균여E-cadherin적저표체화Vimentin적고표체밀절상관(P＜0.05).결론 Twist、HIF-1α화Vimentin재유선암조직중정고표체,이E-cadherin정저표체.HIF-1α가능통과상조Twist표체,촉진상피-간충질전화발생,성위유선암침윤전이적도경지일.
Objective To investigate the expression of Twist and hypoxia inducible factor-1α (HIF-1α) in invasive ductal carcinoma of breast and their relationships with epithelial-mesenchymal transition (EMT).Methods Expression levels of Twist,HIF-1α,E-cadherin and Vimentin in 90 cases of invasive ductal carcinoma tissues and corresponding adjacent tissues were detected by immunohistochemical staining of streptavid-in-peroxidase (SP) method.Results The positive expression rate of Twist,HIF-1 α,E-cadherin and Vimentin was 73.3％,68.9％,15.6％ and 64.4％ respectively in breast cancer tissues,and 7.8％,8.9％,75.6％ and 34.4％ respectively in adjacent tissues.The difference in positive rate between breast cancer tissues and adjacent tissues was statistically significant (P ＜ 0.05).The expression levels of Twist and HIF-1α were correlated with histological grade and lymph node metastasis (P ＜ 0.05).There was a positive correlation between expression levels of Twist and HIF-1α.The expression levels of Twist and HIF-1α were closely correlated with low expression of E-cadherin and high expression of Vimentin.Conclusion Twist,HIF-1α and Vimentin in breast cancer tissues showed higher expression,while E-cadherin lower expression.HIF-1α may promote the occurrence of EMT by up-regulating the expression of Twist,which makes it become one of the possible ways to promote the invasion and metastasis of breast cancer.