CAJ | 학술논문

目的观察喉鳞状细胞癌中K-ras基因启动子甲基化及其蛋白的表达.方法应用甲基化特异性聚合酶链反应(MSP)检测55例喉鳞状细胞癌及其癌旁组织中K-ras基因启动子甲基化状态,并采用Western blot法检测K-ras蛋白的表达.结果 55例喉鳞状细胞癌组织中24例(43.6％) K-ras基因低甲基化,而癌旁组织中仅8例(14.5％)为低甲基化;喉鳞状细胞癌组织中K-ras基因启动子甲基化水平明显低于癌旁组织(P＜0.05);喉鳞状细胞癌组织K-ras基因低甲基化与年龄、性别及淋巴结转移等临床病理特征无明显相关(P＞0.05);而与分化程度及临床分期间明显相关(P＜0.05).此外,喉鳞状细胞癌组织中K-ras蛋白表达水平明显高于癌旁组织.结论 K-ras基因启动子低甲基化可能对喉鳞状细胞癌的发生发展具有重要作用,并可能是喉鳞状细胞癌中K-ras蛋白高表达的原因之一.
목적 관찰후린상세포암중K-ras기인계동자갑기화급기단백적표체.방법 응용갑기화특이성취합매련반응(MSP)검측55례후린상세포암급기암방조직중K-ras기인계동자갑기화상태,병채용Western blot법검측K-ras단백적표체.결과 55례후린상세포암조직중24례(43.6％) K-ras기인저갑기화,이암방조직중부8례(14.5％)위저갑기화;후린상세포암조직중K-ras기인계동자갑기화수평명현저우암방조직(P＜0.05);후린상세포암조직K-ras기인저갑기화여년령、성별급림파결전이등림상병리특정무명현상관(P＞0.05);이여분화정도급림상분기간명현상관(P＜0.05).차외,후린상세포암조직중K-ras단백표체수평명현고우암방조직.결론 K-ras기인계동자저갑기화가능대후린상세포암적발생발전구유중요작용,병가능시후린상세포암중K-ras단백고표체적원인지일.
Objective To investigate the methylation status of K-ras gene promoter and its protein expression in patients with larynx squamous cell carcinoma.Methods The methylation status of K-ras gene promoter in tumor and matched tissue from 55 patients with larynx squamous cell carcinoma was detected by methylation specific polymerease chain reaction (MSP) technique.The relationship between the methylation status of K-ras and the clinicopathological features in patients with larynx squamous cell carcinoma was also analyzed.Furthermore,the protein expression of K-ras was detected by immunoblotting.Results Hypomethylation of K-ras gene promoter was found in 43.6％ (24/55) of the all tumor tissue samples.However,hypomethylation was only found in 8 (14.5％) of 55 matched adjacent non-tumor samples,which was significantly lower than that in tumor tissue samples (P ＜ 0.05).Meanwhile,hypomethylation of K-ras was not obviously related to sex,age,or lymph node metastasis status (P ＞ 0.05).However,hypomethylation of K-ras was significantly correlated with degree of differention and TNM stage (P ＜ 0.05).Moreover,the expression of K-ras in tumor tissue samples was significantly higher than that in paired non-tumor tissue samples (P ＜ 0.05).Conclusion It suggests that hypomethylation of K-ras gene promoter may have an important role in the development of laryngocarcinoma,which also may be one of the reasons for the overexpression of K-ras.