中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
10期
2326-2328,后插2
,共4页
杨冉星%李高峰%俞建军%张笑
楊冉星%李高峰%俞建軍%張笑
양염성%리고봉%유건군%장소
前列腺癌%细胞外信号调节酶5%微小RNA145
前列腺癌%細胞外信號調節酶5%微小RNA145
전렬선암%세포외신호조절매5%미소RNA145
Prostate cancer%Extracellular signal-regulated kinase 5%MicroRNA-145
目的 探讨细胞外信号调节酶5(ERK5)与微小RNA-145(miR-145)在前列腺癌组织中表达及两者之间的关系.方法 分别采用免疫组织化学和原位杂交的方法检测组织芯片上40例前列腺癌患者80点前列腺癌组织以及15点周围正常组织中ERK5与miR-145的表达,并与临床病理特征进行统计学分析.结果 ERK5在前列腺癌组织中阳性表达率为77.5％,而在癌旁组织阳性表达率为26.7％,两者表达差异有统计学意义(P＜0.05).miR-145在前列腺癌组织表达率为27.5％,在癌旁组织阳性表达率为62.5％,两者差异有统计学意义(P＜0.05).ERK5表达与患者的Gleason评分、前列腺特异抗原(PSA)水平、临床分期明显相关(P＜0.05).miR-145在Ⅱ期的前列腺癌患者的表达率明显高于Ⅲ期,ERK5与miR-145在前列腺癌中呈负相关.结论 ERK5与miR-145在前列腺癌中表达呈负相关,提示miR-145与ERK5在前列腺癌发生及进展中密切相关.
目的 探討細胞外信號調節酶5(ERK5)與微小RNA-145(miR-145)在前列腺癌組織中錶達及兩者之間的關繫.方法 分彆採用免疫組織化學和原位雜交的方法檢測組織芯片上40例前列腺癌患者80點前列腺癌組織以及15點週圍正常組織中ERK5與miR-145的錶達,併與臨床病理特徵進行統計學分析.結果 ERK5在前列腺癌組織中暘性錶達率為77.5％,而在癌徬組織暘性錶達率為26.7％,兩者錶達差異有統計學意義(P＜0.05).miR-145在前列腺癌組織錶達率為27.5％,在癌徬組織暘性錶達率為62.5％,兩者差異有統計學意義(P＜0.05).ERK5錶達與患者的Gleason評分、前列腺特異抗原(PSA)水平、臨床分期明顯相關(P＜0.05).miR-145在Ⅱ期的前列腺癌患者的錶達率明顯高于Ⅲ期,ERK5與miR-145在前列腺癌中呈負相關.結論 ERK5與miR-145在前列腺癌中錶達呈負相關,提示miR-145與ERK5在前列腺癌髮生及進展中密切相關.
목적 탐토세포외신호조절매5(ERK5)여미소RNA-145(miR-145)재전렬선암조직중표체급량자지간적관계.방법 분별채용면역조직화학화원위잡교적방법검측조직심편상40례전렬선암환자80점전렬선암조직이급15점주위정상조직중ERK5여miR-145적표체,병여림상병리특정진행통계학분석.결과 ERK5재전렬선암조직중양성표체솔위77.5％,이재암방조직양성표체솔위26.7％,량자표체차이유통계학의의(P＜0.05).miR-145재전렬선암조직표체솔위27.5％,재암방조직양성표체솔위62.5％,량자차이유통계학의의(P＜0.05).ERK5표체여환자적Gleason평분、전렬선특이항원(PSA)수평、림상분기명현상관(P＜0.05).miR-145재Ⅱ기적전렬선암환자적표체솔명현고우Ⅲ기,ERK5여miR-145재전렬선암중정부상관.결론 ERK5여miR-145재전렬선암중표체정부상관,제시miR-145여ERK5재전렬선암발생급진전중밀절상관.
Objective To study the expression of extracellular signal-regulated kinase 5 (ERK5) and microRNA-145 (miR-145) in prostate cancer (PCa) and their correlation.Methods The expression of ERK5 and miR-145 in 80 cases of carcinoma tissues and 15 cases of normal tissues (40 cases of 95 cores in a set of tissue microarrays) was respectively measured by immunohistochemistry and in situ hybridization method.The potential relationship between ERK5 or miR-145 and clinicopathological features was also analyzed.Results ERK5 expression was significantly higher in carcinoma tissues (77.5％) than in normal tissues (26.7％).miR-145 expression was significantly lower in carcinoma tissues (27.5％) than in normal tissues.Overexpression of ERK5 in carcinoma tissues was positively correlated with prostate specific antigen,gleason scores and clinical stages.The lower expression of mirR145 in carcinoma tissues was inversely correlated with clinical stages between Ⅱ and Ⅲ.There was a strong inverse correlation between ERK5 and miR-145.Conclusion The expression of ERK5 and miR-145 in prostate cancer tissues has a strong inverse correlation,which prompts that ERK5 and miR-145 play an important role in prostate cancer development and progression.
