中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2378-2380
,共3页
杨锐%李红波%王兵%邹声泉
楊銳%李紅波%王兵%鄒聲泉
양예%리홍파%왕병%추성천
雷公藤甲素%脂质体%增殖%凋亡%胆管癌
雷公籐甲素%脂質體%增殖%凋亡%膽管癌
뢰공등갑소%지질체%증식%조망%담관암
Triptolide%Liposome%Proliferation%Apoptosis%Cholangiocarcinoma
目的 观察雷公藤甲素(TP)及其脂质体(TP-LP)对人胆管癌细胞株TFK-1增殖和凋亡的影响;比较两者在体内抑制肿瘤生长的作用,探讨脂质体作为TP纳米载体的可行性.方法 采用噻唑蓝(MTT)比色法、平板克隆形成实验、流式细胞术等观察不同浓度的TP及TP-LP对胆管癌细胞TFK-1增殖、周期和凋亡的影响.建立TFK-1细胞种植瘤模型,比较TP及TP-LP对裸鼠种植瘤生长的抑制作用.结果 TP及TP-LP均呈时间、剂量依赖性地抑制TFK-1的增殖,TP-LP对TFK-1的抑制作用优于TP(P ＜0.05).TP和TP-LP均可阻滞TFK1细胞于G0/G1期,100 nmol/L浓度作用48 h后G0/G1期细胞比例分别为(73.26 ±2.56)％、(84.35±3.85)％,差异有统计学意义(P＜0.05).两者均可以诱导TFK1细胞的凋亡,100 nmol/L浓度作用48 h后TFK1细胞凋亡的比例分别为(22.71±3.66)％、(35.23±4.02)％,差异有统计学意义(P＜ 0.05);TFK1裸鼠种植瘤试验中,对照组、TP组和TP-LP组肿瘤体积分别为(1 073.33±20.82)、(306.67±15.28)、(88.0±8.26) mm3,差异有统计学意义(P＜0.05);用药21 d后处死裸鼠,对照组、TP组和TP-LP组荷瘤鼠体质量分别为(24.69±1.53)、(22.85±1.99)、(22.80±1.77)g,3组之间的差异无统计学意义(P＞0.05).结论 TP和TP-LP在体外均能通过阻滞细胞周期和诱导细胞凋亡而抑制TFK-1细胞的增殖,TP-LP的作用强于TP;在体内两者均具有抑制种植瘤生长的作用,TP-LP的抑瘤作用强于TP;TP-LP抗肿瘤的作用强于TP单药,是一种有潜力的新型载药体系.
目的 觀察雷公籐甲素(TP)及其脂質體(TP-LP)對人膽管癌細胞株TFK-1增殖和凋亡的影響;比較兩者在體內抑製腫瘤生長的作用,探討脂質體作為TP納米載體的可行性.方法 採用噻唑藍(MTT)比色法、平闆剋隆形成實驗、流式細胞術等觀察不同濃度的TP及TP-LP對膽管癌細胞TFK-1增殖、週期和凋亡的影響.建立TFK-1細胞種植瘤模型,比較TP及TP-LP對裸鼠種植瘤生長的抑製作用.結果 TP及TP-LP均呈時間、劑量依賴性地抑製TFK-1的增殖,TP-LP對TFK-1的抑製作用優于TP(P ＜0.05).TP和TP-LP均可阻滯TFK1細胞于G0/G1期,100 nmol/L濃度作用48 h後G0/G1期細胞比例分彆為(73.26 ±2.56)％、(84.35±3.85)％,差異有統計學意義(P＜0.05).兩者均可以誘導TFK1細胞的凋亡,100 nmol/L濃度作用48 h後TFK1細胞凋亡的比例分彆為(22.71±3.66)％、(35.23±4.02)％,差異有統計學意義(P＜ 0.05);TFK1裸鼠種植瘤試驗中,對照組、TP組和TP-LP組腫瘤體積分彆為(1 073.33±20.82)、(306.67±15.28)、(88.0±8.26) mm3,差異有統計學意義(P＜0.05);用藥21 d後處死裸鼠,對照組、TP組和TP-LP組荷瘤鼠體質量分彆為(24.69±1.53)、(22.85±1.99)、(22.80±1.77)g,3組之間的差異無統計學意義(P＞0.05).結論 TP和TP-LP在體外均能通過阻滯細胞週期和誘導細胞凋亡而抑製TFK-1細胞的增殖,TP-LP的作用彊于TP;在體內兩者均具有抑製種植瘤生長的作用,TP-LP的抑瘤作用彊于TP;TP-LP抗腫瘤的作用彊于TP單藥,是一種有潛力的新型載藥體繫.
목적 관찰뢰공등갑소(TP)급기지질체(TP-LP)대인담관암세포주TFK-1증식화조망적영향;비교량자재체내억제종류생장적작용,탐토지질체작위TP납미재체적가행성.방법 채용새서람(MTT)비색법、평판극륭형성실험、류식세포술등관찰불동농도적TP급TP-LP대담관암세포TFK-1증식、주기화조망적영향.건립TFK-1세포충식류모형,비교TP급TP-LP대라서충식류생장적억제작용.결과 TP급TP-LP균정시간、제량의뢰성지억제TFK-1적증식,TP-LP대TFK-1적억제작용우우TP(P ＜0.05).TP화TP-LP균가조체TFK1세포우G0/G1기,100 nmol/L농도작용48 h후G0/G1기세포비례분별위(73.26 ±2.56)％、(84.35±3.85)％,차이유통계학의의(P＜0.05).량자균가이유도TFK1세포적조망,100 nmol/L농도작용48 h후TFK1세포조망적비례분별위(22.71±3.66)％、(35.23±4.02)％,차이유통계학의의(P＜ 0.05);TFK1라서충식류시험중,대조조、TP조화TP-LP조종류체적분별위(1 073.33±20.82)、(306.67±15.28)、(88.0±8.26) mm3,차이유통계학의의(P＜0.05);용약21 d후처사라서,대조조、TP조화TP-LP조하류서체질량분별위(24.69±1.53)、(22.85±1.99)、(22.80±1.77)g,3조지간적차이무통계학의의(P＞0.05).결론 TP화TP-LP재체외균능통과조체세포주기화유도세포조망이억제TFK-1세포적증식,TP-LP적작용강우TP;재체내량자균구유억제충식류생장적작용,TP-LP적억류작용강우TP;TP-LP항종류적작용강우TP단약,시일충유잠력적신형재약체계.
Objective To research the effect of triptolide (TP) and triptolide loaded liposome (TP-LP) on proliferation and apoptosis to human cholangiocarcinoma cell line TFK-1,and the inhibition of tumor growth so that we can evaluate the feasibility of liposome as TP nano-carrier.Methods Detected the effect of TP and TP-LP at different concentration level on proliferation,cell cycle and apoptosis to TFK-1 by methyl thiazolyl tetrazolium (MTT),clone formation assay and flow cytometry.In animal study,we constructed TFK-1 node mice xenograft mode to compare the inhibiting effect to mice xenograft of TP and TP-LP.Results TP and TP-LP inhibited proliferation of TFK-1 both time and dose dependent,and TP-LP was superior to TP (P ＜ 0.05).Both TP and TP-LP could block TFK-1 cells in G0/G1 phase and promote them to apoptosis.After treated by TP or TP-LP at 100nmol/L after 48 h,the percentage of G0/G1 phase were (73.26 ± 2.56) ％ and (84.35 ± 3.85) ％ seperately,and the percentage of apoptosis were (22.71 ± 3.66)％ and (35.23 ± 4.02) ％ separately,both of which had significant difference (P ＜ 0.05).The volume of xenograft in control,TP and TP-LP group were (1 073.33 ± 20.82),(306.67 ± 15.28) and (88.0 ±8.26) mm3 seperately,which had significant difference (P ＜0.05).While 21 days after drug-using,the weight of mice in three groups were (24.69 ± 1.53),(22.85 ± 1.99) and (22.80 ± 1.77) g separately,which had no significant difference (P ＞ 0.05).Conclusion Both of TP and TP-LP can inhibit proliferation of TFK-1 by blocking cell cycle and promoting apoptosis in vitro,and inhibit xenograft growth in vivo,while TP-LP is effective.So TP-LP is a potential new drug,which is more effective than TP.
