CAJ | 학술논문

目的观察过表达核心蛋白聚糖(DCN)基因对胆管癌细胞迁移及侵袭功能的影响.方法脂质体介导真核表达质粒pEGFP-DCN和空载体pEGFP-N1转入人胆管癌细胞株QBC939,Western blot检测DCN蛋白以及E-钙黏蛋白的表达,逆转录-聚合酶链反应(RT-PCR)检测DCNmRNA以及E-钙黏蛋白mRNA的表达,Transwell实验检测细胞迁移、侵袭能力.结果 RT-PCR结果显示过表达DCN较pEGFP-N1上调32.34倍;Western blot结果显示过表达组DCN上调2倍.Transwell结果显示转染pEGFP-DCN的胆管癌细胞迁徙和侵袭能力较转染pEGFP-N1分别降低约56.9％和40.2％;转染pEGFP-DCN与pEGFP-N1胆管癌细胞中E-钙黏蛋白的表达水平,结果可见较转染pEGFP-N1体组胆管癌细胞比较,转染pEGFP-DCN组E-钙黏蛋白的表达水平明显升高.结论 DCN过表达可抑制QBC939细胞的迁移与侵袭能力,可能与升高E-钙黏蛋白表达相关.
목적 관찰과표체핵심단백취당(DCN)기인대담관암세포천이급침습공능적영향.방법 지질체개도진핵표체질립pEGFP-DCN화공재체pEGFP-N1전입인담관암세포주QBC939,Western blot검측DCN단백이급E-개점단백적표체,역전록-취합매련반응(RT-PCR)검측DCNmRNA이급E-개점단백mRNA적표체,Transwell실험검측세포천이、침습능력.결과 RT-PCR결과현시과표체DCN교pEGFP-N1상조32.34배;Western blot결과현시과표체조DCN상조2배.Transwell결과현시전염pEGFP-DCN적담관암세포천사화침습능력교전염pEGFP-N1분별강저약56.9％화40.2％;전염pEGFP-DCN여pEGFP-N1담관암세포중E-개점단백적표체수평,결과가견교전염pEGFP-N1체조담관암세포비교,전염pEGFP-DCN조E-개점단백적표체수평명현승고.결론 DCN과표체가억제QBC939세포적천이여침습능력,가능여승고E-개점단백표체상관.
Objective To investigate the effects of decorin (DCN) gene overexpression on the migration and invasion of human cholangiocarcinoma cells.Methods The pEGFP-DCN plasmids and pEGFP-N1 control plasmids were transfected into the human cholangiocarcinoma cell line QBC939 cells.The mRNA and protein expression of decorin and E-cadherin in QBC939 cells transfected with plasmid overexpressing decorin was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively.Transwell assay was used to observe the migration and invasion.Results Compared to the control plasmids,RT-PCR showed the mRNA expression of decorin in the pEGFP-DCN plasmids was increased by 32.34 fold.Western blotting showed the protein expression of decorin was increased by 2 fold.Transwell assay showed that the migration and invasion capacity of the QBC939 cells transfected with pEGFP-DCN was decreased by 56.9％ and 40.2％ respectively as compared with the control cells.The expression level of E-cadherin was significantly increased after DCN transfection into QBC939 cells as compared with the control cells.Conclusion After the over-expression of DCN gene,the migration and invasion capacity of the QBC939 cells was effectively suppressed,which may be associated with vthe elevated E-cadherin expression.