中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2386-2388
,共3页
陈有挺%洪志鹏%周智%欧荣文%石铮
陳有挺%洪誌鵬%週智%歐榮文%石錚
진유정%홍지붕%주지%구영문%석쟁
胆管癌%双氢青蒿素%缺氧诱导因子-1α%体内实验
膽管癌%雙氫青蒿素%缺氧誘導因子-1α%體內實驗
담관암%쌍경청호소%결양유도인자-1α%체내실험
Cholangiocarcinoma%Dihydroartemisinin%Hypoxia-inducible factor-1 α%In vivo study
目的 双氢青蒿素治疗人胆管癌QBC939裸鼠种植瘤的作用并探讨其可能的作用机制.方法 建立人胆管癌QBC939裸鼠种植瘤动物模型,随机分3组:双氢青蒿素组(DHA)、空白对照组(生理盐水)、溶剂对照组(植物油),对比分析各组裸瘤重量、抑瘤率、缺氧诱导因子(HIF)-1α的表达、计数微血管密度(MVD)和凋亡指数(AI).结果 DHA组的种植瘤体积(1 979.63±357.29) mm3]和重量[(1.77±0.23)g]明显小于对照组[种植瘤体积(2 827.69±549.00) mm3,重量(2.50±0.65)g,P＜0.05];实验组的HIF-1α的表达强度(0.2280±0.0439)少于对照组(0.336 7±0.089 6,P＜0.05);实验组的MVD值(11.06±1.44)低于对照组(17.44±2.24,P＜0.05);实验组肿瘤组织的凋亡指数[AI,(13.18±2.42)％]明显大于对照组[(3.28±1.04)％,P＜0.05].结论 双氢青蒿素对人胆管癌裸鼠种植瘤生长具有抑制作用,其作用机制可能与诱导肿瘤细胞凋亡和抑制肿瘤血管生成有关.
目的 雙氫青蒿素治療人膽管癌QBC939裸鼠種植瘤的作用併探討其可能的作用機製.方法 建立人膽管癌QBC939裸鼠種植瘤動物模型,隨機分3組:雙氫青蒿素組(DHA)、空白對照組(生理鹽水)、溶劑對照組(植物油),對比分析各組裸瘤重量、抑瘤率、缺氧誘導因子(HIF)-1α的錶達、計數微血管密度(MVD)和凋亡指數(AI).結果 DHA組的種植瘤體積(1 979.63±357.29) mm3]和重量[(1.77±0.23)g]明顯小于對照組[種植瘤體積(2 827.69±549.00) mm3,重量(2.50±0.65)g,P＜0.05];實驗組的HIF-1α的錶達彊度(0.2280±0.0439)少于對照組(0.336 7±0.089 6,P＜0.05);實驗組的MVD值(11.06±1.44)低于對照組(17.44±2.24,P＜0.05);實驗組腫瘤組織的凋亡指數[AI,(13.18±2.42)％]明顯大于對照組[(3.28±1.04)％,P＜0.05].結論 雙氫青蒿素對人膽管癌裸鼠種植瘤生長具有抑製作用,其作用機製可能與誘導腫瘤細胞凋亡和抑製腫瘤血管生成有關.
목적 쌍경청호소치료인담관암QBC939라서충식류적작용병탐토기가능적작용궤제.방법 건립인담관암QBC939라서충식류동물모형,수궤분3조:쌍경청호소조(DHA)、공백대조조(생리염수)、용제대조조(식물유),대비분석각조라류중량、억류솔、결양유도인자(HIF)-1α적표체、계수미혈관밀도(MVD)화조망지수(AI).결과 DHA조적충식류체적(1 979.63±357.29) mm3]화중량[(1.77±0.23)g]명현소우대조조[충식류체적(2 827.69±549.00) mm3,중량(2.50±0.65)g,P＜0.05];실험조적HIF-1α적표체강도(0.2280±0.0439)소우대조조(0.336 7±0.089 6,P＜0.05);실험조적MVD치(11.06±1.44)저우대조조(17.44±2.24,P＜0.05);실험조종류조직적조망지수[AI,(13.18±2.42)％]명현대우대조조[(3.28±1.04)％,P＜0.05].결론 쌍경청호소대인담관암라서충식류생장구유억제작용,기작용궤제가능여유도종류세포조망화억제종류혈관생성유관.
Objective To investigate the effect and the potential mechanisms of dihydroartemisinin on the treatment of human cholangiocarcinoma cell line QBC939 xenograft in nude mouse.Methods A total of 18 animal models of nude mouse xenograft by human cholangiocarcinoma cell line QBC939 were established and randomly divided into three groups:the dihydroartemisinin group (DHA),the control group (saline),and the solvent control group (vegetable oil).The weight of tumor,the tumor inhibition rate,the expression of hypoxia-inducible factor (HIF)-1α,the microvessel density (MVD) and the apoptotic index (AI) in each group were compared and analyzed.Results The volume and the weight of xenograft tumor in DHA group [(1 979.63 ± 357.29) mm3 and (1.77 ± 0.23) g] were significantly lower than those in the control group [(2 827.69 ± 549.00) mm3 and (2.50 ± 0.65) g,P ＜ 0.05].The expression of HIF-1α in the experimental group (0.228 0 ± 0.043 9)was less than that in the control group (0.336 7 ± 0.089 6,P ＜ 0.05).MVD in the experimental group (11.06 ± 1.44) was less than that in the control group (17.44 ± 2.24,P ＜ 0.05).The AI in the experimental group [(13.18 ± 2.42) ％] was significantly greater than that in the control group [(3.28 ± 1.04) ％,P ＜ 0.05].Conclusion Dihydroartemisinin has an inhibitory effect on the tumor growth of human cholangiocarcinoma in nude mice,and the mechanism may be related to the induction of the apoptosis of tumor cells,and the inhibition of tumor angiogenesis.
