中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2393-2395
,共3页
王百林%苑伟%翟淑萍%程海坤%王蕊%周海玲
王百林%苑偉%翟淑萍%程海坤%王蕊%週海玲
왕백림%원위%적숙평%정해곤%왕예%주해령
梗阻性黄疸%肝细胞%线粒体%氧化应激%丙二醛%8-羟基-2-脱氧鸟苷酸
梗阻性黃疸%肝細胞%線粒體%氧化應激%丙二醛%8-羥基-2-脫氧鳥苷痠
경조성황달%간세포%선립체%양화응격%병이철%8-간기-2-탈양조감산
Obstructive jaundice%Liver cells%Mitochondria%Pxidative damage%Malondialdehyde%8-hydroxydeoxyguanosine
目的 探讨梗阻性黄疸兔肝细胞线粒体氧化损伤的作用机制.方法 选择新西兰白兔30只,按照随机分配的原则分为梗阻性黄疸组24例和对照组6例,造模7d后,分别取各组家兔的肝组织各100 mg,对肝组织内的8-羟基-2-脱氧鸟苷酸(8-OHdG)和丙二醛(MDA)分别进行免疫组织荧光显像、酶联免疫吸附试验(ELISA)法和MDA含量检测.结果 (1)肝组织免疫组织荧光结果:梗阻性黄疸组呈现橘红色,对照组呈现绿色;(2)肝细胞线粒体内8-OHdG的含量:梗阻性黄疸组肝细胞内的8-OHdG的含量(1920 ±231) pg/mg蛋白明显高于对照组(210 ±78) pg/mg蛋白,差异有统计学意义(P<0.01);(3)肝细胞内MDA的含量:梗阻性黄疸组和对照组肝细胞内的MDA分别为(1.08±0.25)和(0.21±0.09) nmol/mg蛋白,前组明显高于后者,差异有统计学意义(P<0.01).结论 兔梗阻性黄疸可导致肝细胞线粒体内氧化应激损伤,其损伤机制可能与肝细胞线粒体内8-OHdG和MDA有关.
目的 探討梗阻性黃疸兔肝細胞線粒體氧化損傷的作用機製.方法 選擇新西蘭白兔30隻,按照隨機分配的原則分為梗阻性黃疸組24例和對照組6例,造模7d後,分彆取各組傢兔的肝組織各100 mg,對肝組織內的8-羥基-2-脫氧鳥苷痠(8-OHdG)和丙二醛(MDA)分彆進行免疫組織熒光顯像、酶聯免疫吸附試驗(ELISA)法和MDA含量檢測.結果 (1)肝組織免疫組織熒光結果:梗阻性黃疸組呈現橘紅色,對照組呈現綠色;(2)肝細胞線粒體內8-OHdG的含量:梗阻性黃疸組肝細胞內的8-OHdG的含量(1920 ±231) pg/mg蛋白明顯高于對照組(210 ±78) pg/mg蛋白,差異有統計學意義(P<0.01);(3)肝細胞內MDA的含量:梗阻性黃疸組和對照組肝細胞內的MDA分彆為(1.08±0.25)和(0.21±0.09) nmol/mg蛋白,前組明顯高于後者,差異有統計學意義(P<0.01).結論 兔梗阻性黃疸可導緻肝細胞線粒體內氧化應激損傷,其損傷機製可能與肝細胞線粒體內8-OHdG和MDA有關.
목적 탐토경조성황달토간세포선립체양화손상적작용궤제.방법 선택신서란백토30지,안조수궤분배적원칙분위경조성황달조24례화대조조6례,조모7d후,분별취각조가토적간조직각100 mg,대간조직내적8-간기-2-탈양조감산(8-OHdG)화병이철(MDA)분별진행면역조직형광현상、매련면역흡부시험(ELISA)법화MDA함량검측.결과 (1)간조직면역조직형광결과:경조성황달조정현귤홍색,대조조정현록색;(2)간세포선립체내8-OHdG적함량:경조성황달조간세포내적8-OHdG적함량(1920 ±231) pg/mg단백명현고우대조조(210 ±78) pg/mg단백,차이유통계학의의(P<0.01);(3)간세포내MDA적함량:경조성황달조화대조조간세포내적MDA분별위(1.08±0.25)화(0.21±0.09) nmol/mg단백,전조명현고우후자,차이유통계학의의(P<0.01).결론 토경조성황달가도치간세포선립체내양화응격손상,기손상궤제가능여간세포선립체내8-OHdG화MDA유관.
Objective To explore the mechanism of rabbit liver mitochondrial oxidative damage about obstructive jaundice.Methods The 30 New Zealand rabbits were divided into two groups,which were the control group and the obstructive jaundice group.After the rabbits were modeled for 7 days,they were taken in liver tissue 100 mg,which were detected the index——8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA).Results (1) The results of fluorescence immunohistochemistry:the liver cells of rabbits in the obstructive jaundice group (pre-treatment) were found to have significant mitochondrial deoxyribonucleicacid (mtDNA) oxidative damage.(2) Levels of 8-OHdG in the liver mitochondria:the 8-OHdG levels of the control group and the obstructive jaundice group were (210 ± 78) pg/mg protein and (1920 ±231) pg/mg protein,There is significant difference between them (P <0.01).(3) Levels of MDA in the liver mitochondria:the MDA levels of the control group and the obstructive jaundice group were (0.21 ± 0.09) nmol/mg protein and (1.08 ± 0.25) nmol/mg protein,There is significant difference between them (P < 0.01).Conclusion The obstructive jaundice of rabbits can cause hepatic mitochondrial oxidative stress injury,which mechanism maybe remove 8-OHdG and MDA in the liver mitochondria.