中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2426-2429
,共4页
朱宁%陈东晖%李付广%郑鹏远
硃寧%陳東暉%李付廣%鄭鵬遠
주저%진동휘%리부엄%정붕원
束缚应激%肠黏膜屏障%大鼠
束縳應激%腸黏膜屏障%大鼠
속박응격%장점막병장%대서
Restraint stress%Intestinal mucosal barrier%Rat
目的 观察束缚应激状态下大脑中动脉线栓(MCAO)模型大鼠肠黏膜屏障的改变,探讨其机制.方法 将雄性SD大鼠48只随机分为假手术组(J组)、假手术+束缚组(JS组)、MCAO模型组(M组)、MCAO模型+束缚组(MS组),每组12只.以改良Longa线栓法制作MCAO模型;以大鼠固定器固定制作束缚应激模型,检测大鼠血清二胺氧化酶(DAO)、D-乳酸(D-LA)、内毒素(LPS)、促肾上腺皮质激素释放因子(CRF)水平,回肠组织紧密连接蛋白水平,回肠组织CRF、CRF-r1、CRF-r2表达水平和脑组织CRF、肿瘤坏死因子(TNF)-α、CRP表达水平.结果 MS组大鼠血清DAO、LPS、CRF水平分别为:(693.96 ±55.38) U/L、(147.04±2.93) μgL、(194.03±19.80) ng/L,与M组比较显著增加(P＜0.05);回肠组织紧密连接蛋白ZO-1、occludin、claudin-1、claudin-2水平均分别为:0.52 ±0.05、0.58 ±0.03、0.57 ±0.05、0.35 ±0.07,与M组比较显著降低(P＜0.05),claudin-3、claudin-4水平分别为:0.59 ±0.40、0.58 ±0.03,与M组比较显著增高(P＜0.05);回肠组织CRF、CRF-r1表达水平及脑组织CRF、CRP表达水平分别为:173.80±34.34、169.56±26.99、115.27±17.79、135.27 ±40.67,与M组比较显著增高(P＜0.05).结论 束缚应激加重MCAO模型大鼠肠黏膜屏障功能的损害.
目的 觀察束縳應激狀態下大腦中動脈線栓(MCAO)模型大鼠腸黏膜屏障的改變,探討其機製.方法 將雄性SD大鼠48隻隨機分為假手術組(J組)、假手術+束縳組(JS組)、MCAO模型組(M組)、MCAO模型+束縳組(MS組),每組12隻.以改良Longa線栓法製作MCAO模型;以大鼠固定器固定製作束縳應激模型,檢測大鼠血清二胺氧化酶(DAO)、D-乳痠(D-LA)、內毒素(LPS)、促腎上腺皮質激素釋放因子(CRF)水平,迴腸組織緊密連接蛋白水平,迴腸組織CRF、CRF-r1、CRF-r2錶達水平和腦組織CRF、腫瘤壞死因子(TNF)-α、CRP錶達水平.結果 MS組大鼠血清DAO、LPS、CRF水平分彆為:(693.96 ±55.38) U/L、(147.04±2.93) μgL、(194.03±19.80) ng/L,與M組比較顯著增加(P＜0.05);迴腸組織緊密連接蛋白ZO-1、occludin、claudin-1、claudin-2水平均分彆為:0.52 ±0.05、0.58 ±0.03、0.57 ±0.05、0.35 ±0.07,與M組比較顯著降低(P＜0.05),claudin-3、claudin-4水平分彆為:0.59 ±0.40、0.58 ±0.03,與M組比較顯著增高(P＜0.05);迴腸組織CRF、CRF-r1錶達水平及腦組織CRF、CRP錶達水平分彆為:173.80±34.34、169.56±26.99、115.27±17.79、135.27 ±40.67,與M組比較顯著增高(P＜0.05).結論 束縳應激加重MCAO模型大鼠腸黏膜屏障功能的損害.
목적 관찰속박응격상태하대뇌중동맥선전(MCAO)모형대서장점막병장적개변,탐토기궤제.방법 장웅성SD대서48지수궤분위가수술조(J조)、가수술+속박조(JS조)、MCAO모형조(M조)、MCAO모형+속박조(MS조),매조12지.이개량Longa선전법제작MCAO모형;이대서고정기고정제작속박응격모형,검측대서혈청이알양화매(DAO)、D-유산(D-LA)、내독소(LPS)、촉신상선피질격소석방인자(CRF)수평,회장조직긴밀련접단백수평,회장조직CRF、CRF-r1、CRF-r2표체수평화뇌조직CRF、종류배사인자(TNF)-α、CRP표체수평.결과 MS조대서혈청DAO、LPS、CRF수평분별위:(693.96 ±55.38) U/L、(147.04±2.93) μgL、(194.03±19.80) ng/L,여M조비교현저증가(P＜0.05);회장조직긴밀련접단백ZO-1、occludin、claudin-1、claudin-2수평균분별위:0.52 ±0.05、0.58 ±0.03、0.57 ±0.05、0.35 ±0.07,여M조비교현저강저(P＜0.05),claudin-3、claudin-4수평분별위:0.59 ±0.40、0.58 ±0.03,여M조비교현저증고(P＜0.05);회장조직CRF、CRF-r1표체수평급뇌조직CRF、CRP표체수평분별위:173.80±34.34、169.56±26.99、115.27±17.79、135.27 ±40.67,여M조비교현저증고(P＜0.05).결론 속박응격가중MCAO모형대서장점막병장공능적손해.
Objective To investigate the effect of restrain stress on intestinal mucosal barrier function in middle cerebral artery occulusion (MCAO) rats and its mechanism.Methods Male SD rats were randomly divided into sham group (J),sham-operated plus restraint group (JS),MCAO group (M),and MACO plus restraint group (MS) (n =12 each).Improved Longa suture method was used to establish MCAO model in rats,and rat fixator to creat restraint stress model.Serum diamineoxidase (DAO),D-lactic acid (D-LA),endotoxin (LPS),and corticotropin releasing factor (CRF) levels,tight junction proteins in ileum tissue,CRF,CRF-r1 and CRF-r2 expression levels in ileum tissue,and CRF,tumor necrosis factor (TNF)-α and CRP expression levels in the brain tissues were measured.Results The serum DAO,LPS and CRF levels in MS group were (693.96 ± 55.38) U/L,(147.04 ± 2.93) μg/L,and (194.03 ± 19.80) ng/L respectively,significantly higher than in M group (all P ＜ 0.05).The levels of tight junction proteins ZO-1,occluding,claudin-1 and claudin-2 levels in the ileum tissue of MS group were 0.52 ± 0.05,0.58 ± 0.03,0.57 ± 0.05 and 0.35 ± 0.07 respectively,significantly higher than in M group (all P ＜ 0.05).The expression levels of claudin-3 and claudin-4 in MS group were 0.59 ± 0.40 and 0.58 ± 0.03 respectively,significantly higher than M group (all P ＜ 0.05).The CRF and CRF-r1 expression levels in ileum tissue,and CRF and CRP expression levels in brain tissues of MS group were 173.80 ± 34.34,169.56 ± 26.99,115.27 ± 17.79 and 135.27 ± 40.67 respectively,significantly higher than M group (all P ＜ 0.05).Conclusion Restrain stress aggravated intestinal mucosa barrier damage in MCAO rats.
