CAJ | 학술논문

目的观察音猬因子(Shh)信号通路在小鼠急性胰腺炎中的作用.方法雨蛙素腹腔注射诱导急性胰腺炎模型后,引入Hedgehog信号的拮抗剂环杷明(Cyclopamine),用逆转录-聚合酶链反应(RT-PCR)、Western blot方法检测Shh在胰腺组织的表达,同时检测了胰腺髓过氧化物酶(MPO)水平.结果与对照组比较,雨蛙素注射1h后,Shh表达逐渐升高,在24h到达最高.与对照组比较[(231.13 ±10.03) U/mg],MPO活性逐渐升高,6h到达高峰[(1 255.56±126.68) U/mg],24h恢复正常[(230.48±17.69) U/mg].当Cyelopamine抑制Hedgehog信号后,胰腺炎症明显加重.结论在急性胰腺炎性过程中,Shh起重要的保护作用.
목적 관찰음위인자(Shh)신호통로재소서급성이선염중적작용.방법 우와소복강주사유도급성이선염모형후,인입Hedgehog신호적길항제배파명(Cyclopamine),용역전록-취합매련반응(RT-PCR)、Western blot방법검측Shh재이선조직적표체,동시검측료이선수과양화물매(MPO)수평.결과 여대조조비교,우와소주사1h후,Shh표체축점승고,재24h도체최고.여대조조비교[(231.13 ±10.03) U/mg],MPO활성축점승고,6h도체고봉[(1 255.56±126.68) U/mg],24h회복정상[(230.48±17.69) U/mg].당Cyelopamine억제Hedgehog신호후,이선염증명현가중.결론 재급성이선염성과정중,Shh기중요적보호작용.
Objective To study the potential role of Sonic Hedgehog (Shh) signal pathway in mice acute pancreatitis.Methods Acute pancreatitis was induced by intraperitoneal injections of cerulean 7 times at hourly intervals in mice,and the specific antagonist of Hedgehog was injected as well.Shh expression was detected by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC).The level of pancreatic myeloperoxidase (MPO) was determined.Results As compared with control group,RT-PCR and Western blotting results showed Shh in experimental group was increased,at 24 h,up to peak values.Inflammation was assessed by the activity of MPO.In control group,the MPO activity was (231.13 ± 10.03) U/mg,and experimental group showed a rapid increase in MPO activity,at 6 h,up to peak values [(1 255.56 ± 126.68) U/mg].The MPO activity recovered baseline at 24 h [(230.48 ± 17.69) U/mg].When hedgehog signal was inhibited by cyclopamine,the inflammation of pancreas was more serious.Conclusion During the acute pancreatitis,Shh signal may play a protective role to regulate the pancreatic inflammation.