中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2453-2455
,共3页
吕晓涓%韩娜%张孟贤%董震
呂曉涓%韓娜%張孟賢%董震
려효연%한나%장맹현%동진
预防性脑照射%脑转移灶%小鼠黑色素瘤%血管新生%活体生物荧光发光%生存分析
預防性腦照射%腦轉移竈%小鼠黑色素瘤%血管新生%活體生物熒光髮光%生存分析
예방성뇌조사%뇌전이조%소서흑색소류%혈관신생%활체생물형광발광%생존분석
Prophylactic cranial irradiation%Brain metastasis%Murine melanoma%Angiogenesis%In vivo bioluminescence%Survival analysis
目的 观察预防性脑照射(PCI)对小鼠黑色素瘤脑转移灶生长和血管新生的影响并探讨其机制.方法 PCI组小鼠在颅内种植肿瘤1周前接受25 Gy的全脑照射,对照组小鼠接受伪照射.立体定向微量注射法建立C57/BL6小鼠同源性黑色素瘤B16F10Luc2颅内转移瘤.活体生物荧光发光技术定量测定颅内的肿瘤负荷,并进行生存分析.免疫荧光组织化学测定并比较两组肿瘤组织、对侧正常脑组织的CD31和CD105标记的微血管密度(MVD)和微血管面积(MVA).结果 肿瘤颅内种植后第10天PCI组颅内转移瘤活体荧光强度明显低于对照组(P＜0.05);生存分析显示PCI组动物中位数存活时间(1Sd)显著长于对照组(16 d)(P＜0.05);两组的肿瘤组织内的MVD[对照组(10.933±3.058)个/0.148mm2,PCI组(13.067±3.150)个/0.148mm2]均显著低于对侧脑组织[对照组(17.200±2.859)个/0.148 mm2,PCI组(16.267±2.434)个/0.148 mm2,P＜0.05],PCI组的CD105-MVD[(0.867 ±0.990)个/0.148 mm2]及CD31-MVA[(1.622 ±0.440)％]都显著低于对照组[CD105-MVD:(2.200±1.146)个/0.148 mm2,CD31-MVA:(2.219 ±0.712)％,P＜0.05].结论 PCI对小鼠黑色素瘤颅内转移灶生长和非出芽性血管新生有显著的抑制作用,其机制有待进一步研究.
目的 觀察預防性腦照射(PCI)對小鼠黑色素瘤腦轉移竈生長和血管新生的影響併探討其機製.方法 PCI組小鼠在顱內種植腫瘤1週前接受25 Gy的全腦照射,對照組小鼠接受偽照射.立體定嚮微量註射法建立C57/BL6小鼠同源性黑色素瘤B16F10Luc2顱內轉移瘤.活體生物熒光髮光技術定量測定顱內的腫瘤負荷,併進行生存分析.免疫熒光組織化學測定併比較兩組腫瘤組織、對側正常腦組織的CD31和CD105標記的微血管密度(MVD)和微血管麵積(MVA).結果 腫瘤顱內種植後第10天PCI組顱內轉移瘤活體熒光彊度明顯低于對照組(P＜0.05);生存分析顯示PCI組動物中位數存活時間(1Sd)顯著長于對照組(16 d)(P＜0.05);兩組的腫瘤組織內的MVD[對照組(10.933±3.058)箇/0.148mm2,PCI組(13.067±3.150)箇/0.148mm2]均顯著低于對側腦組織[對照組(17.200±2.859)箇/0.148 mm2,PCI組(16.267±2.434)箇/0.148 mm2,P＜0.05],PCI組的CD105-MVD[(0.867 ±0.990)箇/0.148 mm2]及CD31-MVA[(1.622 ±0.440)％]都顯著低于對照組[CD105-MVD:(2.200±1.146)箇/0.148 mm2,CD31-MVA:(2.219 ±0.712)％,P＜0.05].結論 PCI對小鼠黑色素瘤顱內轉移竈生長和非齣芽性血管新生有顯著的抑製作用,其機製有待進一步研究.
목적 관찰예방성뇌조사(PCI)대소서흑색소류뇌전이조생장화혈관신생적영향병탐토기궤제.방법 PCI조소서재로내충식종류1주전접수25 Gy적전뇌조사,대조조소서접수위조사.입체정향미량주사법건립C57/BL6소서동원성흑색소류B16F10Luc2로내전이류.활체생물형광발광기술정량측정로내적종류부하,병진행생존분석.면역형광조직화학측정병비교량조종류조직、대측정상뇌조직적CD31화CD105표기적미혈관밀도(MVD)화미혈관면적(MVA).결과 종류로내충식후제10천PCI조로내전이류활체형광강도명현저우대조조(P＜0.05);생존분석현시PCI조동물중위수존활시간(1Sd)현저장우대조조(16 d)(P＜0.05);량조적종류조직내적MVD[대조조(10.933±3.058)개/0.148mm2,PCI조(13.067±3.150)개/0.148mm2]균현저저우대측뇌조직[대조조(17.200±2.859)개/0.148 mm2,PCI조(16.267±2.434)개/0.148 mm2,P＜0.05],PCI조적CD105-MVD[(0.867 ±0.990)개/0.148 mm2]급CD31-MVA[(1.622 ±0.440)％]도현저저우대조조[CD105-MVD:(2.200±1.146)개/0.148 mm2,CD31-MVA:(2.219 ±0.712)％,P＜0.05].결론 PCI대소서흑색소류로내전이조생장화비출아성혈관신생유현저적억제작용,기궤제유대진일보연구.
Objective To study the impact of prophylactic cranial irradiation (PCI) on growth and angiogenesis of murine brain metastasis of syngeneic melanoma.Methods One week prior to implantation,eight mice from prophylactic cranial irradiation (PCI) group received PCI with total dosage of 25 Gy in 10 fractions,while eight mice from control group only received sham irradiation.The intracranial metastases of B16F10Luc2 melanoma syngeneic to C57/BL6 mice were established with stereotactic micro-injection implantation.In vivo bioluminescence,representing intracranial tumor burden,were quantitatively measured and survival analysis was carried out.Microvessel density (MVD) and microvessel area(MVA) labelled by anti-CD31 and anti-CD105 staining were analyzed.Results In vivo fluorescence intensity of PCI group was significant lower than control group (P ＜ 0.05) on the 10th day post-implantation.Survival analysis showed that the median survival time of PCI group (18 days) was longer than control group (16 days) (P ＜ 0.05).MVD within tumor tissue in both groups[(10.933 ± 3.058)/0.148 mm2] were significantly lower than the contralateral brain tissue [(17.200 ± 2.859)/0.148 mm2 for control group and (16.267 ± 2.434)/0.148 mm2 for PCI group,P ＜ 0.05].CD105-MVD [(0.867 ± 0.990)/0.148 mm2] and CD31-MVA[(1.622 ± 0.440)％] within tumor of PCI group were significantly lower than control group [CD105-MVD:(2.200 ± 1.146)/0.148 mm2,CD31-MVA:(2.219 ± 0.712) ％,P ＜ 0.05].Conclusion PCI might significantly suppress the growth of intracranial metastasis of murine melanoma through inhibiting tumor non-spouting angiogenesis.Further investigations of the mechanism are warranted.
