中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2548-2550
,共3页
杜培准%李宇%周岩冰%吕亮%谭斌%孙腾%焦学龙%周金哲
杜培準%李宇%週巖冰%呂亮%譚斌%孫騰%焦學龍%週金哲
두배준%리우%주암빙%려량%담빈%손등%초학룡%주금철
甲状腺乳头状癌%长链非编码RNA%芯片
甲狀腺乳頭狀癌%長鏈非編碼RNA%芯片
갑상선유두상암%장련비편마RNA%심편
Thyroid papillary carcinoma%Long non-cording RNA%Microarray
目的 建立甲状腺乳头状癌组织与癌旁组织中长链非编码RNA (lncRNA)差异表达谱.方法 分别提取5例甲状腺乳头状癌患者癌组织和癌旁组织中的RNA,体外逆转录制备并标记双链DNA,用含有39000条lncRNA的双通道基因芯片检测,对原始数据进行归一化处理及差异分析.结果 芯片筛选出差异表达的lncRNA 26 924条.有明显差异的lncRNA共245条(变化>2倍,P <0.05),这其中在肿瘤组织中高表达的IncRNA 73条,低表达的lncRNA 172条.变化在3倍以上的lncRNA共40条,差异在4倍以上的lncRNA有2条.结论 甲状腺乳头状癌组织与癌旁组织比较,其lncRNA表达谱发生明显改变,提示lncRNA可能参与甲状腺乳头状癌的发生.
目的 建立甲狀腺乳頭狀癌組織與癌徬組織中長鏈非編碼RNA (lncRNA)差異錶達譜.方法 分彆提取5例甲狀腺乳頭狀癌患者癌組織和癌徬組織中的RNA,體外逆轉錄製備併標記雙鏈DNA,用含有39000條lncRNA的雙通道基因芯片檢測,對原始數據進行歸一化處理及差異分析.結果 芯片篩選齣差異錶達的lncRNA 26 924條.有明顯差異的lncRNA共245條(變化>2倍,P <0.05),這其中在腫瘤組織中高錶達的IncRNA 73條,低錶達的lncRNA 172條.變化在3倍以上的lncRNA共40條,差異在4倍以上的lncRNA有2條.結論 甲狀腺乳頭狀癌組織與癌徬組織比較,其lncRNA錶達譜髮生明顯改變,提示lncRNA可能參與甲狀腺乳頭狀癌的髮生.
목적 건립갑상선유두상암조직여암방조직중장련비편마RNA (lncRNA)차이표체보.방법 분별제취5례갑상선유두상암환자암조직화암방조직중적RNA,체외역전록제비병표기쌍련DNA,용함유39000조lncRNA적쌍통도기인심편검측,대원시수거진행귀일화처리급차이분석.결과 심편사선출차이표체적lncRNA 26 924조.유명현차이적lncRNA공245조(변화>2배,P <0.05),저기중재종류조직중고표체적IncRNA 73조,저표체적lncRNA 172조.변화재3배이상적lncRNA공40조,차이재4배이상적lncRNA유2조.결론 갑상선유두상암조직여암방조직비교,기lncRNA표체보발생명현개변,제시lncRNA가능삼여갑상선유두상암적발생.
Objective To analysis the expression profile variation of long non-cording RNA (lncRNA) in thyroid papillary carcinoma and normal thyroid tissue.Methods Total RNA from the thyroid papillary carcinoma tissue and normal thyroid tissue of 5 patients with thyroid papillary were prepared respectively.Hybridization was performed with the profile chip containing 39 000 human lncRNAs.After the scanning of Agilent scanner,data were normalized and analyzed by using the Agilent GeneSpring software.Results Compared with normal thyroid tissue,26 923 lncRNAs expressed differentially,245 lncRNAs were significantly differential (fold change > 2 ; P < 0.5) in thyroid papillary carcinoma tissue,in which 73 lncRNAs were up-regulation and 172 lncRNAs were down-regulation.Conclusion Obvious changes of lncRNA expression profile were observed in the pathogenesis of thyroid papillary carcinoma,lncRNA may be related to the progress of thyroid papillary carcinoma.