CAJ | 학술논문

目的探讨结直肠腺癌遗传易感性与多药耐药基因(MDR1)多态性的关系.方法通过多重单碱基延伸反应(SnaPshot SNP分型技术)检测123例结直肠腺癌患者及386例健康对照者MDR1基因中C3435T、G2677T/A等位基因频率及基因型频率.分析正常人与结直肠腺癌患者C3435T、G2677T/A等位基因频率及基因型频率的差异.结果在正常人及结肠腺癌患者中,C3435T、G2677T/A等位基因频率及基因型频率总体分布差异无统计学意义(P＞0.05).结直肠腺癌组按性别、年龄、病变部位、分化程度、Dukes分期等临床病理特征分组后,C3435T基因型频率分布在结肠癌(CC 22.2％,CT 63.4％,TT 14.8％)和直肠癌(CC 50.7％,CT 31.9％,TT17.4％)患者中差异有统计学意义(P＜0.01),等位基因3435T在直肠癌患者中的分布频率(33.3％)较结肠癌(46.3％)显著降低(P＜0.05).与2677GG比较,2677GT基因型频率在中等分化腺癌(26.9％)中的分布较高分化(46.4％)和低分化腺癌(47.1％)显著降低(P＜0.05),2677GT基因型频率在结肠癌患者(46.3％)中较直肠癌患者(24.6％)占明显优势(P＜0.05).2677G等位基因在低分化腺癌(3.7％)的分布频率较中分化(28.5％)和高分化(11.0％)腺癌显著降低(P＜0.05).结论 C3435T、G2677T/A位点基因多态性与结直肠腺癌无明显相关.
목적 탐토결직장선암유전역감성여다약내약기인(MDR1)다태성적관계.방법 통과다중단감기연신반응(SnaPshot SNP분형기술)검측123례결직장선암환자급386례건강대조자MDR1기인중C3435T、G2677T/A등위기인빈솔급기인형빈솔.분석정상인여결직장선암환자C3435T、G2677T/A등위기인빈솔급기인형빈솔적차이.결과 재정상인급결장선암환자중,C3435T、G2677T/A등위기인빈솔급기인형빈솔총체분포차이무통계학의의(P＞0.05).결직장선암조안성별、년령、병변부위、분화정도、Dukes분기등림상병리특정분조후,C3435T기인형빈솔분포재결장암(CC 22.2％,CT 63.4％,TT 14.8％)화직장암(CC 50.7％,CT 31.9％,TT17.4％)환자중차이유통계학의의(P＜0.01),등위기인3435T재직장암환자중적분포빈솔(33.3％)교결장암(46.3％)현저강저(P＜0.05).여2677GG비교,2677GT기인형빈솔재중등분화선암(26.9％)중적분포교고분화(46.4％)화저분화선암(47.1％)현저강저(P＜0.05),2677GT기인형빈솔재결장암환자(46.3％)중교직장암환자(24.6％)점명현우세(P＜0.05).2677G등위기인재저분화선암(3.7％)적분포빈솔교중분화(28.5％)화고분화(11.0％)선암현저강저(P＜0.05).결론 C3435T、G2677T/A위점기인다태성여결직장선암무명현상관.
Objective To investigate the association between genetic polymorphism in exon 26 C3435T and exon 21 G2677T/A of the multidrug resistance 1 (MDR1) gene and the sporadic colorectal adenocarcinoma.Methods A total of 123 patients with colorectal adenocarcinoma and 86 healthy subjects as controls were included.Snapshot SNP typing was used to determine the allele and genotype frequencies of MDR1 C3435T,and G2677T/A.Results There was no significant difference in the distribution of MDR1 C3435T and G2677T/A alleles and genotypes frequencies between control group and case group.When stratified by clinicopathological features such as lesion distribution,differentiation degree and Duke' s stage in the patients with colorectal adenocarcinoma,the statistically significant differences were found in the frequency of C3435T genotype between patients with colon adenocarcinoma (CC 22.2％,CT 63.4％,and TT 14.8 ％) and rectal adenocarcinoma (CC 50.7％,CT 31.9％,and TT 17.4％) (P ＜ 0.01).The frequency of 3435T allele was decreased significantly in patiens with rectal adenocarcinoma (33.3％) as compared with colon adenocarcinoma (46.3％,P ＜0.05).And the frequency of 2677GT genotype was decreased significantly in patients with moderately-differentiated (26.9％) as compared with well-differentiated (46.4％) and poorly-differentiated (47.1％,P ＜ 0.05).Furthermore,the frequency of 2677GT genotype was increased significantly in patients with colon adenocarcinoma (46.3％) as compared with rectal adenocarcinoma (24.6％,P ＜ 0.05),and the frequency of 2677G allele was decreased significantly in patiens with poorly differentiated adenocarcinoma (3.7％) as compared with moderately-differentiated (28.5％) and well-differentiated adenocarcinoma (11.0％,P ＜ 0.05).Conclusion MDR1 C3435T and G2677T/A polymorphisms were found to be not associated with colorectal adenocarcinoma.