中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
11期
2596-2597
,共2页
罗小锋%李仕军%郑育民%黄天德%邵林%冯茂辉
囉小鋒%李仕軍%鄭育民%黃天德%邵林%馮茂輝
라소봉%리사군%정육민%황천덕%소림%풍무휘
肺癌%糖皮质激素诱导蛋白激酶1
肺癌%糖皮質激素誘導蛋白激酶1
폐암%당피질격소유도단백격매1
Lung carcinoma%Serum and glucocorticoid induced protein kinase 1 gene
目的 观察人肺癌组织中糖皮质激素诱导蛋白激酶1(SGK1)基因的表达.方法 应用逆转录-聚合酶链反应(RT-PCR)方法检测10例中晚期肺癌及其癌旁组织中SGK1 mRNA的相对表达量,并采用Western blot检测上述组织中SGK1蛋白表达.结果 70％(7/10)的肺癌组织中SGK1 mRNA和蛋白表达下调或缺失,而癌旁组织中80％ (8/10)该基因表达上调和蛋白表达增加,两者差异有统计学意义(P＜0.05).结论 肺癌组织中SGK1表达下调,可能在肺癌的发生、发展过程中发挥重要作用.
目的 觀察人肺癌組織中糖皮質激素誘導蛋白激酶1(SGK1)基因的錶達.方法 應用逆轉錄-聚閤酶鏈反應(RT-PCR)方法檢測10例中晚期肺癌及其癌徬組織中SGK1 mRNA的相對錶達量,併採用Western blot檢測上述組織中SGK1蛋白錶達.結果 70％(7/10)的肺癌組織中SGK1 mRNA和蛋白錶達下調或缺失,而癌徬組織中80％ (8/10)該基因錶達上調和蛋白錶達增加,兩者差異有統計學意義(P＜0.05).結論 肺癌組織中SGK1錶達下調,可能在肺癌的髮生、髮展過程中髮揮重要作用.
목적 관찰인폐암조직중당피질격소유도단백격매1(SGK1)기인적표체.방법 응용역전록-취합매련반응(RT-PCR)방법검측10례중만기폐암급기암방조직중SGK1 mRNA적상대표체량,병채용Western blot검측상술조직중SGK1단백표체.결과 70％(7/10)적폐암조직중SGK1 mRNA화단백표체하조혹결실,이암방조직중80％ (8/10)해기인표체상조화단백표체증가,량자차이유통계학의의(P＜0.05).결론 폐암조직중SGK1표체하조,가능재폐암적발생、발전과정중발휘중요작용.
Objective To observe the expression of glucocorticoid-induced protein kinase 1 (SGK1) gene in human lung carcinoma.Methods The samples were obtained from 10 cases of lung carcinoma.Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used for analyzing the SGK1 mRNA and protein expression in 10 samples and adjacent noncanerous tissues.Results SGK1 mRNA and protein expression was not detected in 70％ of human lung carcinoma and 20％ adjacent noncanerous tissues (P ＜ 0.05).Conclusion SGK1 expression is downregulated in human lung carcinoma tissue,which may play some roles in the pathogenesis of human lung carcinoma.
