CAJ | 학술논문

目的建立一种稳定的马斯特里赫特Ⅱ类心脏死亡捐献模型.方法选取巴马小型猪12头,以静脉推注氯化钾的方法诱导心跳骤停,继而通过心肺复苏机给予30 min标准心脏按压,5 min不接触时间之内无心电活动及自主呼吸,宣布死亡.建立模型过程中持续监测生命体征、肝脏血流,并检测肝脏功能、血气分析及肝脏病理变化.结果所有实验猪静脉推注氯化钾后出现室颤,心肺复苏过程中平均动脉压迅速降至30 mmHg(1 mmHg=0.133 kPa)以下,肝脏血流100 ～140 ml/min,为基础值的14％～ 20％.宣布死亡时pH值显著降低,乳酸显著升高,病理检查提示肝细胞出现肝窦阻塞,弥漫性肝细胞水样变性.结论静脉推注氯化钾,继而给予心肺复苏的方法可以建立一种稳定的马斯特里赫特Ⅱ类心脏死亡捐献低血压模型.
목적 건립일충은정적마사특리혁특Ⅱ류심장사망연헌모형.방법 선취파마소형저12두,이정맥추주록화갑적방법유도심도취정,계이통과심폐복소궤급여30 min표준심장안압,5 min불접촉시간지내무심전활동급자주호흡,선포사망.건립모형과정중지속감측생명체정、간장혈류,병검측간장공능、혈기분석급간장병리변화.결과 소유실험저정맥추주록화갑후출현실전,심폐복소과정중평균동맥압신속강지30 mmHg(1 mmHg=0.133 kPa)이하,간장혈류100 ～140 ml/min,위기출치적14％～ 20％.선포사망시pH치현저강저,유산현저승고,병리검사제시간세포출현간두조새,미만성간세포수양변성.결론 정맥추주록화갑,계이급여심폐복소적방법가이건립일충은정적마사특리혁특Ⅱ류심장사망연헌저혈압모형.
Objective To establish a stable Maastricht type Ⅱ donation after cardiac death model.Methods Cardiac arrest was induced in 12 mini-pigs by administration of 1 g KCL intravenously,followed by 30 minutes cardiopulmonary resuscitation (CPR) according to standard guideline.Then death was declared after 5 minutes of "no-touch" time without cardiac activities and spontaneous respiration.During this process,vital signs and hepatic flow were monitored continuously,and liver biochemistry,arterial blood gas analysis and biopsy were taken intermittently.Results Ventricular fibrillation was induced immediately after the administration of intravenous KCL.Mean arterial blood pressure during CPR was below 30 mmHg (1 mmHg =0.133 kPa),with hepatic flow of 100-140 ml/min (14％-20％ of the basic flow).pH decreased significantly at the time of cardiac death with lactate increasing significantly,and liver biopsy indicated congested hepatic sinusoids and diffuse hydropic changes in the hepatocytes.Conclusion We successfully established a Maastricht type Ⅱ donation after cardiac death model in pig with intravenous KCL and CPR method.