中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2012年
10期
780-784
,共5页
王彩丽%田园青%刘丽萍%贾妮亚%南蕾
王綵麗%田園青%劉麗萍%賈妮亞%南蕾
왕채려%전완청%류려평%가니아%남뢰
肌球蛋白重链9%多态性,单核苷酸%IgA肾病
肌毬蛋白重鏈9%多態性,單覈苷痠%IgA腎病
기구단백중련9%다태성,단핵감산%IgA신병
Myosin heavy chain 9%Polymorphism,single nucleotide%IgA nephropathy
目的 探讨肌球蛋白重链(MYH)9基因单核苷酸多态性与内蒙古自治区汉族IgA肾病患者临床特征、病理及预后的关系.方法 以经肾组织活检确诊的IgA肾病患者148例为研究对象,对其中56例患者进行了1～97月的随访.取外周血提取DNA,采用PCR限制性片段长度多态性分析(RFLP)法检测MYH9基因Rs3752462、Rs4821480位点单核苷酸多态性.研究各位点基因型与IgA肾病患者临床特征的相关性.分析不同基因型与疾病进展和预后的关系.结果 (1)Rs3752462位点符合Hardy-Weinberg平衡,Rs4821480位点不符合Hardy-Weinberg平衡.(2)IgA肾病患者MYH9基因Rs3752462位点TT基因型患者的收缩压低于CC+CT基因型(P＜0.05).Rs4821480位点GG基因型与TT+GT基因型两组患者收缩压、舒张压、年龄差异有统计学意义(P＜0.05).Scr、肌酐清除率、血白蛋白、血红蛋白、镜下血尿、蛋白尿程度等临床指标及病理HASS分级、肾病理改变在Rs4821480位点、Rs3752462位点3种基因型组间差异无统计学意义.(3) Kaplan-Meier生存分析提示Rs3752462位点CC基因型、Rs4821480位点TT基因型患者肾活检到肾功能减退时间显著较短(P＜0.05).结论 MYH9基因Rs3752462位点C等位基因是引起IgA肾病患者高血压损害的独立危险因素.MYH9基因Rs4821480位点3种基因型多态性与患者预后相关.携带Rs3752462位点C等位基因、Rs4821480位点T等位基因可能影响患者的预后.
目的 探討肌毬蛋白重鏈(MYH)9基因單覈苷痠多態性與內矇古自治區漢族IgA腎病患者臨床特徵、病理及預後的關繫.方法 以經腎組織活檢確診的IgA腎病患者148例為研究對象,對其中56例患者進行瞭1～97月的隨訪.取外週血提取DNA,採用PCR限製性片段長度多態性分析(RFLP)法檢測MYH9基因Rs3752462、Rs4821480位點單覈苷痠多態性.研究各位點基因型與IgA腎病患者臨床特徵的相關性.分析不同基因型與疾病進展和預後的關繫.結果 (1)Rs3752462位點符閤Hardy-Weinberg平衡,Rs4821480位點不符閤Hardy-Weinberg平衡.(2)IgA腎病患者MYH9基因Rs3752462位點TT基因型患者的收縮壓低于CC+CT基因型(P＜0.05).Rs4821480位點GG基因型與TT+GT基因型兩組患者收縮壓、舒張壓、年齡差異有統計學意義(P＜0.05).Scr、肌酐清除率、血白蛋白、血紅蛋白、鏡下血尿、蛋白尿程度等臨床指標及病理HASS分級、腎病理改變在Rs4821480位點、Rs3752462位點3種基因型組間差異無統計學意義.(3) Kaplan-Meier生存分析提示Rs3752462位點CC基因型、Rs4821480位點TT基因型患者腎活檢到腎功能減退時間顯著較短(P＜0.05).結論 MYH9基因Rs3752462位點C等位基因是引起IgA腎病患者高血壓損害的獨立危險因素.MYH9基因Rs4821480位點3種基因型多態性與患者預後相關.攜帶Rs3752462位點C等位基因、Rs4821480位點T等位基因可能影響患者的預後.
목적 탐토기구단백중련(MYH)9기인단핵감산다태성여내몽고자치구한족IgA신병환자림상특정、병리급예후적관계.방법 이경신조직활검학진적IgA신병환자148례위연구대상,대기중56례환자진행료1～97월적수방.취외주혈제취DNA,채용PCR한제성편단장도다태성분석(RFLP)법검측MYH9기인Rs3752462、Rs4821480위점단핵감산다태성.연구각위점기인형여IgA신병환자림상특정적상관성.분석불동기인형여질병진전화예후적관계.결과 (1)Rs3752462위점부합Hardy-Weinberg평형,Rs4821480위점불부합Hardy-Weinberg평형.(2)IgA신병환자MYH9기인Rs3752462위점TT기인형환자적수축압저우CC+CT기인형(P＜0.05).Rs4821480위점GG기인형여TT+GT기인형량조환자수축압、서장압、년령차이유통계학의의(P＜0.05).Scr、기항청제솔、혈백단백、혈홍단백、경하혈뇨、단백뇨정도등림상지표급병리HASS분급、신병리개변재Rs4821480위점、Rs3752462위점3충기인형조간차이무통계학의의.(3) Kaplan-Meier생존분석제시Rs3752462위점CC기인형、Rs4821480위점TT기인형환자신활검도신공능감퇴시간현저교단(P＜0.05).결론 MYH9기인Rs3752462위점C등위기인시인기IgA신병환자고혈압손해적독립위험인소.MYH9기인Rs4821480위점3충기인형다태성여환자예후상관.휴대Rs3752462위점C등위기인、Rs4821480위점T등위기인가능영향환자적예후.
Objective To study the association of MYH9 gene single nucleotide polymorphism (SNP) with clinical manifestation,pathology and prognosis of IgA nephropathy (IgAN) patients of Han nationality population in Inner Mongolia Autonomous Region.Method One hundred and forty-eight IgAN patients proven by biopsy were enrolled in the study.Fifty-six patients were followed up for 1-97 months.DNA was extracted from the peripheral blood of above patients.PCR restriction fragment length polymorphism (RFLP) assay was used to detect the single nucleotide polymorphisms of MYH9 gene Rs3752462,Rs4821480 sites.Association of different genotypes with clinical features,pathology and prognosis im patients with IgA nephropathy was examined.Result (1) Rs3752462 site was consistent with Hardy-Weinberg equilibrium,while Rs4821480 site did not meet the Hardy-Weinberg equilibrium.(2) IgAN patients with MYH9 gene Rs3752462 site TF genotype had lower systolic blood pressure as compared to those with CC +CT genotype (P＜0.05).There were significant differences in systolic blood pressure,diastolic blood pressure and age between patients with Rs4821480 site GG genotype and patients with TT or GT genotype (P＜0.05).There were no significant differences in Scr,Ccr,plasma albumin,hemoglobin,microscopic hematuria,proteinuria,pathological HASS classification,pathological lesion among Rs4821480 site GG,TT,GT genotypes.(3) Kaplan-Meier survival analysis revealed the time from renal biopsy to renal function decline was shorted in patients with Rs3752462 site CC genotype and Rs4821480 site TT genotype.Conclusions C allele of MYH9 gene Rs3752462 site is an independent risk factor of high blood pressure damage in IgAN patients.Polymorphism of 3 genotypes of MYH9 gene Rs4821480 site is associated to the prognosis of patients.Carrying Rs3752462 site C allele and Rs4821480 site T allele may affect the prognosis of patients.
