中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2012年
10期
804-807
,共4页
臧秀娟%宫丽%洪海娟%姜燕%郑峰%刘梅%于青
臧秀娟%宮麗%洪海娟%薑燕%鄭峰%劉梅%于青
장수연%궁려%홍해연%강연%정봉%류매%우청
再灌注损伤%细胞凋亡%肾小管上皮细胞%凋亡蛋白%中性粒细胞明胶酶相关脂质运载蛋白
再灌註損傷%細胞凋亡%腎小管上皮細胞%凋亡蛋白%中性粒細胞明膠酶相關脂質運載蛋白
재관주손상%세포조망%신소관상피세포%조망단백%중성립세포명효매상관지질운재단백
Reperfusion injury%Apoptosis%Renal tubular epithelial cells%Apoptosis-regulated protein%Neutrophil gelatinase associated lipocalin
目的 探讨中性粒细胞明胶酶相关脂质运载蛋白(NGAL)对大鼠缺血再灌注损伤肾脏肾小管上皮细胞凋亡的保护作用及机制.方法 建立大鼠肾脏缺血再灌注模型,雄性SD大鼠随机分为对照组、缺血再灌注模型组、NGAL组 ;HE染色观察3组大鼠肾组织病理变化 ;TUNEL法检测肾小管上皮细胞凋亡 ;实时定量PCR、Western印迹法检测凋亡蛋白fas、bcl-2的表达变化.结果 与缺血再灌注模型组比较,NGAL组肾小管上皮细胞凋亡数量显著减少[(8.6±3.4)/HP比(20.8±3.7)/HP,P＜0.05] ;NGAL组肾组织fas mRNA(2.34±0.51比6.84±2.34,P＜0.05)、fas蛋白(0.65±0.05比0.95±0.08,P＜0.05)表达显著下调,bcl-2蛋白(0.33±0.05比0.24±0.03,P＜0.05)表达显著上调,但bcl-2 mRNA表达无明显改变.结论 NGAL对大鼠缺血再灌注损伤肾小管上皮细胞有保护作用,其作用可能与减少细胞凋亡、改变凋亡蛋白的表达有关.
目的 探討中性粒細胞明膠酶相關脂質運載蛋白(NGAL)對大鼠缺血再灌註損傷腎髒腎小管上皮細胞凋亡的保護作用及機製.方法 建立大鼠腎髒缺血再灌註模型,雄性SD大鼠隨機分為對照組、缺血再灌註模型組、NGAL組 ;HE染色觀察3組大鼠腎組織病理變化 ;TUNEL法檢測腎小管上皮細胞凋亡 ;實時定量PCR、Western印跡法檢測凋亡蛋白fas、bcl-2的錶達變化.結果 與缺血再灌註模型組比較,NGAL組腎小管上皮細胞凋亡數量顯著減少[(8.6±3.4)/HP比(20.8±3.7)/HP,P＜0.05] ;NGAL組腎組織fas mRNA(2.34±0.51比6.84±2.34,P＜0.05)、fas蛋白(0.65±0.05比0.95±0.08,P＜0.05)錶達顯著下調,bcl-2蛋白(0.33±0.05比0.24±0.03,P＜0.05)錶達顯著上調,但bcl-2 mRNA錶達無明顯改變.結論 NGAL對大鼠缺血再灌註損傷腎小管上皮細胞有保護作用,其作用可能與減少細胞凋亡、改變凋亡蛋白的錶達有關.
목적 탐토중성립세포명효매상관지질운재단백(NGAL)대대서결혈재관주손상신장신소관상피세포조망적보호작용급궤제.방법 건립대서신장결혈재관주모형,웅성SD대서수궤분위대조조、결혈재관주모형조、NGAL조 ;HE염색관찰3조대서신조직병리변화 ;TUNEL법검측신소관상피세포조망 ;실시정량PCR、Western인적법검측조망단백fas、bcl-2적표체변화.결과 여결혈재관주모형조비교,NGAL조신소관상피세포조망수량현저감소[(8.6±3.4)/HP비(20.8±3.7)/HP,P＜0.05] ;NGAL조신조직fas mRNA(2.34±0.51비6.84±2.34,P＜0.05)、fas단백(0.65±0.05비0.95±0.08,P＜0.05)표체현저하조,bcl-2단백(0.33±0.05비0.24±0.03,P＜0.05)표체현저상조,단bcl-2 mRNA표체무명현개변.결론 NGAL대대서결혈재관주손상신소관상피세포유보호작용,기작용가능여감소세포조망、개변조망단백적표체유관.
Objective To investigate the effects of neutrophil gelatinase associated lipocalin(NGAL) on renal tubular epithelial cells apoptosis and apoptosis-regulated protein fas,bcl-2 in rat renal ischemia reperfusion injury (IRI).Methods Renal IRI models of rats were established.Rats were randomly divided into 3 groups,including control group,IRI model group and NGAL group.The pathological change of kidney tissue was investigated by hemotoxylin-eosin staining.Renal tubular epithelial cells apoptosis was detected by TUNEL method.Expression of fas and bcl-2 was measured by real-time PCR and Western blotting.Results Compared with IRI model group,NGAL group showed a decreased number of renal tubular epithelial cells apoptosis [(8.6±3.4)/HP vs (20.8±3.7)/HP,P＜0.05],down-regulated fas mRNA (2.34±0.51 vs 6.84±2.34,P＜ 0.05),fas protein (0.65±0.05 vs 0.95±0.08,P＜0.05) and up-regulated bcl-2 protein (0.33±0.05 vs 0.24±0.03,P＜0.05),but the bcl-2 mRNA had no significant change.Conclusion NGAL can protect renal tubular epithelial cells in renal IRI,which may be associated with decreasing cell apoptosis and adjusting protein expression by apoptosis-regulated cytokines.
