中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2012年
11期
840-844
,共5页
边琪%郭志勇%胡海燕%李娟
邊琪%郭誌勇%鬍海燕%李娟
변기%곽지용%호해연%리연
蜀葵属%足细胞%肾病%黄蜀葵花
蜀葵屬%足細胞%腎病%黃蜀葵花
촉규속%족세포%신병%황촉규화
Althaea%Podocytes%Nephrosis%Sunset Abelmoschus
目的 探讨黄蜀葵花(黄葵)治疗阿霉素肾病大鼠的疗效及其对足细胞病变的影响.方法 雄性SD大鼠50只,按随机数字法分为假手术组(n=10)、模型组(n=10)、黄葵低剂量组(0.5 g·kg-1·d-1,n=10)、黄葵中剂量组(1.0 g·kg-1·d-1,n=10)和黄葵高剂量组(2.0 g·kg-1·d-1,n=10).采用单侧肾切除联合两次阿霉素(ADR)注射法,制备阿霉素肾病大鼠模型.黄葵各组于右肾摘除术当天起给予相应剂量黄葵溶液灌胃.分别在术前、术后2、4、6、8周末检测大鼠尿蛋白、尿N-乙酰葡萄糖氨基转移酶(NAG)、血清白蛋白、Scr和血脂.第8周末宰杀大鼠,取肾组织行光镜和电镜检查,并观察肾组织nephrin的分布.结果 与模型组比较,黄葵各组在各时间点的尿蛋白量和尿NAG水平均降低,以高剂量组最显著(P＜0.01);且血浆白蛋白增加,血脂紊乱改善,差异均有统计学意义(P＜0.05).模型组及黄葵各治疗组Scr自第4周起较假手术组明显升高；第8周末,黄葵高剂量组Scr低于模型组(P＜0.05).黄葵各组肾小球球性硬化和节段硬化比例均低于模型组,肾小管间质损害改善,且以高剂量组最显著.与模型组比较,黄葵各组足细胞的足突损伤减轻,足突融合程度和范围均有所改善,以高剂量组最显著.黄葵各组肾组织nephrin表达较模型组增加.结论 黄葵能减少阿霉素肾病大鼠的蛋白尿,减轻肾组织损伤和慢性化,其机制可能与改善足细胞病变有关.
目的 探討黃蜀葵花(黃葵)治療阿黴素腎病大鼠的療效及其對足細胞病變的影響.方法 雄性SD大鼠50隻,按隨機數字法分為假手術組(n=10)、模型組(n=10)、黃葵低劑量組(0.5 g·kg-1·d-1,n=10)、黃葵中劑量組(1.0 g·kg-1·d-1,n=10)和黃葵高劑量組(2.0 g·kg-1·d-1,n=10).採用單側腎切除聯閤兩次阿黴素(ADR)註射法,製備阿黴素腎病大鼠模型.黃葵各組于右腎摘除術噹天起給予相應劑量黃葵溶液灌胃.分彆在術前、術後2、4、6、8週末檢測大鼠尿蛋白、尿N-乙酰葡萄糖氨基轉移酶(NAG)、血清白蛋白、Scr和血脂.第8週末宰殺大鼠,取腎組織行光鏡和電鏡檢查,併觀察腎組織nephrin的分佈.結果 與模型組比較,黃葵各組在各時間點的尿蛋白量和尿NAG水平均降低,以高劑量組最顯著(P＜0.01);且血漿白蛋白增加,血脂紊亂改善,差異均有統計學意義(P＜0.05).模型組及黃葵各治療組Scr自第4週起較假手術組明顯升高；第8週末,黃葵高劑量組Scr低于模型組(P＜0.05).黃葵各組腎小毬毬性硬化和節段硬化比例均低于模型組,腎小管間質損害改善,且以高劑量組最顯著.與模型組比較,黃葵各組足細胞的足突損傷減輕,足突融閤程度和範圍均有所改善,以高劑量組最顯著.黃葵各組腎組織nephrin錶達較模型組增加.結論 黃葵能減少阿黴素腎病大鼠的蛋白尿,減輕腎組織損傷和慢性化,其機製可能與改善足細胞病變有關.
목적 탐토황촉규화(황규)치료아매소신병대서적료효급기대족세포병변적영향.방법 웅성SD대서50지,안수궤수자법분위가수술조(n=10)、모형조(n=10)、황규저제량조(0.5 g·kg-1·d-1,n=10)、황규중제량조(1.0 g·kg-1·d-1,n=10)화황규고제량조(2.0 g·kg-1·d-1,n=10).채용단측신절제연합량차아매소(ADR)주사법,제비아매소신병대서모형.황규각조우우신적제술당천기급여상응제량황규용액관위.분별재술전、술후2、4、6、8주말검측대서뇨단백、뇨N-을선포도당안기전이매(NAG)、혈청백단백、Scr화혈지.제8주말재살대서,취신조직행광경화전경검사,병관찰신조직nephrin적분포.결과 여모형조비교,황규각조재각시간점적뇨단백량화뇨NAG수평균강저,이고제량조최현저(P＜0.01);차혈장백단백증가,혈지문란개선,차이균유통계학의의(P＜0.05).모형조급황규각치료조Scr자제4주기교가수술조명현승고；제8주말,황규고제량조Scr저우모형조(P＜0.05).황규각조신소구구성경화화절단경화비례균저우모형조,신소관간질손해개선,차이고제량조최현저.여모형조비교,황규각조족세포적족돌손상감경,족돌융합정도화범위균유소개선,이고제량조최현저.황규각조신조직nephrin표체교모형조증가.결론 황규능감소아매소신병대서적단백뇨,감경신조직손상화만성화,기궤제가능여개선족세포병변유관.
Objective To explore the effect of Sunset Abelmoschus on podocyte injury in adriamycin-induced nephropathy rats.Methods Fifty male SD rats were randomly divided into five groups:sham operation group (n=10),model group (n=10),Sunset Abelmoschus low dose group (0.5 g·kg-1· d-1 n=10),middle dose group (1.0 g· kg-1· d-1,n=10) and high dose group (2.0 g· kg-1· d-1,n=10).Unilateral nephrectomy combined repeated adriamycin injection were performed to establish adriamycininduced nephropathy models.The rats were administered with the corresponding dose of Sunset Abelmoschus during the experiment period.Urinary protein,urinary N-acetyl glucose aminotransferase (NAG),serum albumin,serum creatinine and blood lipid were measured before operation and 2,4,6,8 weeks after operation.The rats were sacrificed on week 8 for the renal histological examination,including light microscope and electron microscope.Expression of nephrin was examined by immunofluorescence assays.Results As compared to model group,urinary protein and NAG significantly decreases in Sunset Abelmoschus groups in each time point,especially in high dose group (P＜0.01),meanwhile the serum albumin increased and the disturbance of lipid metabolism was improved in Sunset Abelmoschus groups (P＜0.05).Compared with sham group,Scr increased significantly in model group and Sunset Abelmoschus groups at the 4th week.At the 8th week,Scr in high dose group was lower than that in model group (P＜0.05),and the ratio of glomerular globe sclerosis and segmental sclerosis,tubulointerstitial damage reduced in Sunset Abelmoschus groups,especially in high dose group.The podocyte damage and the extent of foot process fusion were improved in Sunset Abelmoschus groups compared with model group.Expression of nephrin increased in Sunset Abelmoschus groups than that in model group.Conclusion Sunset Abelmoschus can ameliorate proteinuria and renal tissue damage of adriamycin-induced nephropathy rats,whose mechanism may be associated with the improvement of podocyte injury.
