CAJ | 학술논문

目的研究M型磷脂酶A2受体(PLA2R)的基因多态性与中国东北汉族人群特发性膜性肾病(IMN)的相关性.方法应用聚合酶链反应-限制性片段长度多态性(PCRRFLP)技术检测95例IMN患者(IMN组)及232例健康体检者(HC组)PLA2R基因rs35771982和rs3828323两个位点的基因型和等位基因频率.结果 IMN组和HC组间性别、体质量指数(BMI)相匹配.IMN组的平均年龄、Scr、总胆固醇(TC)和24 h尿蛋白量均显著高于HC组(均P＜ 0.01)；血清Alb和eGFR显著低于HC组(P＜0.01).IMN组rs35771982位点CC基因型和C等位基因的频率均显著高于HC组(x2=13.658,P=0.001；x2=15.315,P=9.10×10-5).而两组间rs3828323位点基因型和等位基因频率的差异无统计学意义.rs35771982位点CC基因型与年龄、性别、BMI、血压、血清Alb、TC、Scr、eGFR及24 h尿蛋白量等指标无相关性.rs35771982位点基因型、年龄、TC、Scr及eGFR与IMN的发病相关,rs35771982位点的CC基因型是IMN的危险因素(OR=4.408,95％ CI 1.488～ 13.058).结论中国汉族人群PLA2R rs35771982位点基因多态性可能与IMN易感性相关,而rs3828323位点基因多态性与IMN无相关.rs35771982位点CC基因型是IMN的危险因素.
목적 연구M형린지매A2수체(PLA2R)적기인다태성여중국동북한족인군특발성막성신병(IMN)적상관성.방법 응용취합매련반응-한제성편단장도다태성(PCRRFLP)기술검측95례IMN환자(IMN조)급232례건강체검자(HC조)PLA2R기인rs35771982화rs3828323량개위점적기인형화등위기인빈솔.결과 IMN조화HC조간성별、체질량지수(BMI)상필배.IMN조적평균년령、Scr、총담고순(TC)화24 h뇨단백량균현저고우HC조(균P＜ 0.01)；혈청Alb화eGFR현저저우HC조(P＜0.01).IMN조rs35771982위점CC기인형화C등위기인적빈솔균현저고우HC조(x2=13.658,P=0.001；x2=15.315,P=9.10×10-5).이량조간rs3828323위점기인형화등위기인빈솔적차이무통계학의의.rs35771982위점CC기인형여년령、성별、BMI、혈압、혈청Alb、TC、Scr、eGFR급24 h뇨단백량등지표무상관성.rs35771982위점기인형、년령、TC、Scr급eGFR여IMN적발병상관,rs35771982위점적CC기인형시IMN적위험인소(OR=4.408,95％ CI 1.488～ 13.058).결론 중국한족인군PLA2R rs35771982위점기인다태성가능여IMN역감성상관,이rs3828323위점기인다태성여IMN무상관.rs35771982위점CC기인형시IMN적위험인소.
Objective To investigate the correlation of M-type phospholipase A2 receptor (PLA2R) genetic polymorphism in two single nucleotide polymorphisms (SNPs) with idiopathic membranous nephropathy (IMN) of Chinese Han population in Northeast China.Methods A total of 327 individuals were enrolled in the study including 95 adult patients with biopsy-proved IMN (IMN group) followed up for (25.4±11.6) months and 232 healthy people identified by healthy examination in China-Japan Union Hospital of Jilin University (HC group).Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotype and allele frequency of rs35771982 and rs3828323 site in PLA2R gene.The x2 test was performed to compare the distribution difference of allelic frequency and genotype frequency of the two sites in PLA2R gene between two groups.Unconditional Logistic regression analysis was used to determine the risk factor of IMN.Results IMN and HC group were matched in male predominance and body mass index (BMI).Patients with IMN were older than the healthy controls and had higher Scr,serum total cholesterol (TC),24-hour urine protein level and lower serum albumin (Alb) level,lower estimated glomerular filtration rate (eGFR) than the healthy controls (all P ＜ 0.01).The CC genotype frequency and the C allele frequency at SNP rs35771982 site of PLA2R gene in IMN group were significantly higher than those in HC group (x2=13.658,P=0.001;x2=15.315,P=9.10× 10-5),whereas there was no distribution difference of genotype and allele frequency at rs3828323 site between two groups (x2=2.844,P =0.241; x2 =2.959,P=0.085).The CC genotype at rs35771982 site in patients with IMN was not related to,age,gender,BMI,blood pressure and several laboratory indexes such as Alb,TC,Scr,eGFR and 24-hour urine protein level (all P ＞ 0.05).Unconditional Logistic regression analysis revealed that the genotype at rs35771982,age,TC,Scr and eGFR were correlated with IMN occurrence.The CC genotype at rs35771982 was the risk factor of IMN (OR =4.408,95％CI 1.488-13.058).Conclusions The CC genotype and C allele at rs35771982 site in PLA2R may be associated with the susceptibility to IMN,whereas the correlation between gene polymorphism at rs3828323 site and IMN is not demonstrated.The CC genotype at rs35771982 is the independent risk factor of IMN in Chinese Han population in Northeast China.