CAJ | 학술논문

目的观察缺血再灌注(IR)大鼠肾组织凝血纤溶相关物质的蛋白表达并探讨其意义.方法雄性Wistar大鼠48只被随机分为6组:假手术组(Sham组)、缺血再灌注0、2、6、12、24 h组(IR0h组、IR2h组、IR6h组、IR 12h组、IR 24 h组),每组8只.用无创动脉夹夹闭双肾动脉45 min建立IR模型.HE染色观察肾组织病理改变；免疫组织化学法检测肾组织内皮素(ET-1)、组织型纤溶酶原激活物(tPA)、纤维蛋白原样蛋白2(Fgl-2)凝血酶原酶蛋白的表达.结果与Sham组相比,IR 24 h组大鼠血BUN及Scr升高,差异有统计学意义(P＜0.05).IR各组肾组织病理改变程度随再灌注时间延长而逐渐加重；与Sham组相比,IR各组肾小管损伤半定量评分明显升高,差异有统计学意义(P＜0.05).IR各组肾ET-1蛋白表达增加,6h达高峰,与Sham组相比差异有统计学意义(P＜0.05).IR各组肾Fgl-2凝血酶原酶表达增加,12h达高峰,24 h基本恢复正常,与Sham组相比,差异有统计学意义(P＜0.05).肾组织t-PA表达增加,12h达高峰,与Sham组相比,差异有统计学意义(P＜0.05).结论肾脏IR损伤后存在肾组织凝血纤溶相关物质的异常表达,推测后者可能参与了肾脏IR损伤.
목적 관찰결혈재관주(IR)대서신조직응혈섬용상관물질적단백표체병탐토기의의.방법 웅성Wistar대서48지피수궤분위6조:가수술조(Sham조)、결혈재관주0、2、6、12、24 h조(IR0h조、IR2h조、IR6h조、IR 12h조、IR 24 h조),매조8지.용무창동맥협협폐쌍신동맥45 min건립IR모형.HE염색관찰신조직병리개변；면역조직화학법검측신조직내피소(ET-1)、조직형섬용매원격활물(tPA)、섬유단백원양단백2(Fgl-2)응혈매원매단백적표체.결과 여Sham조상비,IR 24 h조대서혈BUN급Scr승고,차이유통계학의의(P＜0.05).IR각조신조직병리개변정도수재관주시간연장이축점가중；여Sham조상비,IR각조신소관손상반정량평분명현승고,차이유통계학의의(P＜0.05).IR각조신ET-1단백표체증가,6h체고봉,여Sham조상비차이유통계학의의(P＜0.05).IR각조신Fgl-2응혈매원매표체증가,12h체고봉,24 h기본회복정상,여Sham조상비,차이유통계학의의(P＜0.05).신조직t-PA표체증가,12h체고봉,여Sham조상비,차이유통계학의의(P＜0.05).결론 신장IR손상후존재신조직응혈섬용상관물질적이상표체,추측후자가능삼여료신장IR손상.
Objective To investigate the expression and significance of related substances of coagulation and fibrinolysis in the rat kidneys with ischemia reperfusion (IR) injury.Methods Forty-eight male Wistar rats were randomly divided into six groups:Sham,IR 0 h,IR 2 h,IR 6 h,IR 12 h and IR 24 h.IR rats were made by occlusion of bilateral renal arteries by bulldog clamp for 45 minutes.Renal pathology was observed by HE staining.The expression of endothelin-1 (ET-1),tissue plasminogen activator (t-PA) and fibrinogen-like protein 2 (Fgl-2) of renal tissue was detected by immunohistochemical method.Results Scr and BUN increased significantly in IR 24 h group compared with sham group(P＜0.05).Renal pathological injury was significantly aggravated with prolonged reperfusion.The semi-quantitative scores of renal tubular injury were increased in all IR group compared with sham group(P＜0.05).The expression of ET-1 was gradually increased before the first six hour after IR and decreased after twelve hour.Compared with sham group,ET-1 was increased significantly in all IR group.The expression of Fgl-2 fibroleukin in renal tissue increased progressively in the first six hour after IR,weaken in IR 12 h group and trended to normal in IR 24 h group.The expression of t-PA increased progressively during the first six hour after IR,maintained this level in IR 12 h group and decreased in IR 24 h group.Conclusion The abnormal expression of related substances of coagulation and fibrinolysis of rats may participate in renal IR injury.