中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2013年
8期
611-615
,共5页
张盼%魏萌%蒋红利%任怡%史珂慧%王斐倩
張盼%魏萌%蔣紅利%任怡%史珂慧%王斐倩
장반%위맹%장홍리%임이%사가혜%왕비천
尿毒症%炎症%细菌移位%菌群失调
尿毒癥%炎癥%細菌移位%菌群失調
뇨독증%염증%세균이위%균군실조
Uremia%Inflammation%Bacterial translocation%Microbiome dysbiosis
目的 探讨尿毒症大鼠是否发生肠道菌群失调和肠道菌群移位以及肠道菌群移位与微炎性反应的关系.方法 建立大鼠5/6肾切除尿毒症模型及假手术模型.PCR扩增血、肝、脾、肠系膜淋巴结中细菌16SrDNA；设计血液、肝、脾中细菌特异性引物,扩增结肠内容物、肠系膜淋巴结中细菌16SrDNA片段；焦磷酸测序分析两组大鼠回肠及结肠内容物菌群谱.检测两组大鼠超敏C反应蛋白(hs-CRP)、白细胞介素6(IL-6)水平及肠道通透性.结果 部分尿毒症大鼠个体血液、肝、脾、肠系膜淋巴结检测到细菌DNA.血液、肝、脾中细菌特异性引物可扩增相同个体肠系膜淋巴结及结肠内容物的细菌16SrDNA.尿毒症组结肠菌群谱与假手术组在目、科、属水平存在差异.尿毒症组hs-CRP、IL-6、肠道通透性均高于假手术组(均P＜0.05).结论 尿毒症大鼠肠道菌群失调,推测肠道细菌移位诱发微炎性反应.
目的 探討尿毒癥大鼠是否髮生腸道菌群失調和腸道菌群移位以及腸道菌群移位與微炎性反應的關繫.方法 建立大鼠5/6腎切除尿毒癥模型及假手術模型.PCR擴增血、肝、脾、腸繫膜淋巴結中細菌16SrDNA；設計血液、肝、脾中細菌特異性引物,擴增結腸內容物、腸繫膜淋巴結中細菌16SrDNA片段；焦燐痠測序分析兩組大鼠迴腸及結腸內容物菌群譜.檢測兩組大鼠超敏C反應蛋白(hs-CRP)、白細胞介素6(IL-6)水平及腸道通透性.結果 部分尿毒癥大鼠箇體血液、肝、脾、腸繫膜淋巴結檢測到細菌DNA.血液、肝、脾中細菌特異性引物可擴增相同箇體腸繫膜淋巴結及結腸內容物的細菌16SrDNA.尿毒癥組結腸菌群譜與假手術組在目、科、屬水平存在差異.尿毒癥組hs-CRP、IL-6、腸道通透性均高于假手術組(均P＜0.05).結論 尿毒癥大鼠腸道菌群失調,推測腸道細菌移位誘髮微炎性反應.
목적 탐토뇨독증대서시부발생장도균군실조화장도균군이위이급장도균군이위여미염성반응적관계.방법 건립대서5/6신절제뇨독증모형급가수술모형.PCR확증혈、간、비、장계막림파결중세균16SrDNA；설계혈액、간、비중세균특이성인물,확증결장내용물、장계막림파결중세균16SrDNA편단；초린산측서분석량조대서회장급결장내용물균군보.검측량조대서초민C반응단백(hs-CRP)、백세포개소6(IL-6)수평급장도통투성.결과 부분뇨독증대서개체혈액、간、비、장계막림파결검측도세균DNA.혈액、간、비중세균특이성인물가확증상동개체장계막림파결급결장내용물적세균16SrDNA.뇨독증조결장균군보여가수술조재목、과、속수평존재차이.뇨독증조hs-CRP、IL-6、장도통투성균고우가수술조(균P＜0.05).결론 뇨독증대서장도균군실조,추측장도세균이위유발미염성반응.
Objective To investigate whether gut microbiome dysbiosis and translocation occurred in experimental uremia,and whether they consequently contribute to microinflammation.Methods Health male SD rats were randomly divided into uremic group and sham group.Uremic group were operated for 5/6 nephrectomy to establish uremic models,while sham group were only operated for nephrocapsulotomy.Postoperative blood,livers,spleens,and mesenteric lymph nodes (MLNs) were subjected to bacterial 16S ribosomal DNA amplification to determine if bacteria were present.Bacterial genomic DNA samples from the MLNs and colon were amplified with specific primers designed by the 16SrRNA sequence of the species obtained from blood,livers and spleens.Pyrosequencing was used to analyze the ileum and colonic microbio.me of each subject.Intestinal permeability to 99mTc-DTPA,plasma hs-CRP,and IL-6 were measured.Results Bacterial DNA in extraiutestinal sites and altered colonic microbiomes at the phylum,family,and genus levels were detected in some rats in the uremic group.Bacterial genomic DNA in MLNs and colon were obtained by primers specific for bacterial species observed from blood,livers,and spleens of identical individuals.Intestinal permeability,plasma hs-CRP,and IL-6 levels were statistically higher in the uremic group compared with that in sham group(all P ＜ 0.05).Conclusion Gut microbiome dysbiosis occurs and presumably bacteria translocate to the systemic and lymph circulation,thereby contributing to microinflammation in experimental uremia.
