中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2013年
5期
274-277
,共4页
王晴晴%朴梅花%王新利%韩彤妍%张娟%关育红%齐婧
王晴晴%樸梅花%王新利%韓彤妍%張娟%關育紅%齊婧
왕청청%박매화%왕신리%한동연%장연%관육홍%제청
胰岛素样生长因子Ⅰ%启动区(遗传学)%多态现象,遗传%婴儿,新生
胰島素樣生長因子Ⅰ%啟動區(遺傳學)%多態現象,遺傳%嬰兒,新生
이도소양생장인자Ⅰ%계동구(유전학)%다태현상,유전%영인,신생
Insulin-like growth factor Ⅰ%Promoter regions (genetics)%Polymorphism,genetic%Infant,newborn
目的 检测新生儿胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)启动子区域737/738位点胞嘧啶腺苷(cytosine-adenosine,CA)重复序列的基因多态性. 方法 选择2010年6月1日至2012年6月30日于北京大学第三医院出生的健康新生儿561例.记录新生儿的性别、出生胎龄、出生体重、身长,并进行胎龄评估.出生后3～5 d清晨空腹采集足跟血,提取DNA,分析IGF-1启动子区域基因多态性.采用x2检验、独立样本t检验、方差分析和Hardy-Weinberg平衡检验进行统计学分析. 结果 561例新生儿中,足月儿413例,早产儿148例；大于胎龄儿92例,适于胎龄儿433例,小于胎龄儿36例.561例共发现7种不同的等位基因及23种不同的基因型.7种等位基因分别为188、190、192、194、196、198和200 bp.最常见的3种基因型为190～192 bp、192～196 bp及192～192 bp,其频率分别为23.2％(130/561)、15.0％(84/561)和12.8％(72/561).CA19纯合基因型(CA19/CA19)、CA19杂合基因型(CA19/CAno19)及CA19突变基因型(CAno19/CAno19)的频率在足月儿和早产儿之间差异均无统计学意义[分别为11.4％(47/413)与16.9％(25/148)、55.9％(231/413)与50.7％(75/148)、32.7％(135/413)与32.4％(48/148),x2=2.96、1.21和0.00,P均＞0.05].CA19/CA19、CA19/CAno19、CAno19/CAno19这3种基因型新生儿的出生胎龄分别为(37.1±2.9)、(37.6±3.1)和(37.4±3.1)周,差异无统计学意义(F=0.54,P=0.58).CA19/CA19在小于胎龄儿中的频率明显高于大于胎龄儿及适于胎龄儿[分别为25.0％(9/36)、7.6％(7/92)及12.9％(56/433),x2=7.01,P=0.03],但CA19/CAno19及CAno19/CAno19基因型频率在大于胎龄儿、适于胎龄儿及小于胎龄儿3组之间差异无统计学意义(CA19/CAno19:x2=1.13,P=0.57；CAno19/CAno19:x2=0.58,P=0.75). 结论 IGF-1启动子区存在基因多态性,CA19等位基因频率与出生胎龄无关.
目的 檢測新生兒胰島素樣生長因子-1(insulin-like growth factor-1,IGF-1)啟動子區域737/738位點胞嘧啶腺苷(cytosine-adenosine,CA)重複序列的基因多態性. 方法 選擇2010年6月1日至2012年6月30日于北京大學第三醫院齣生的健康新生兒561例.記錄新生兒的性彆、齣生胎齡、齣生體重、身長,併進行胎齡評估.齣生後3～5 d清晨空腹採集足跟血,提取DNA,分析IGF-1啟動子區域基因多態性.採用x2檢驗、獨立樣本t檢驗、方差分析和Hardy-Weinberg平衡檢驗進行統計學分析. 結果 561例新生兒中,足月兒413例,早產兒148例；大于胎齡兒92例,適于胎齡兒433例,小于胎齡兒36例.561例共髮現7種不同的等位基因及23種不同的基因型.7種等位基因分彆為188、190、192、194、196、198和200 bp.最常見的3種基因型為190～192 bp、192～196 bp及192～192 bp,其頻率分彆為23.2％(130/561)、15.0％(84/561)和12.8％(72/561).CA19純閤基因型(CA19/CA19)、CA19雜閤基因型(CA19/CAno19)及CA19突變基因型(CAno19/CAno19)的頻率在足月兒和早產兒之間差異均無統計學意義[分彆為11.4％(47/413)與16.9％(25/148)、55.9％(231/413)與50.7％(75/148)、32.7％(135/413)與32.4％(48/148),x2=2.96、1.21和0.00,P均＞0.05].CA19/CA19、CA19/CAno19、CAno19/CAno19這3種基因型新生兒的齣生胎齡分彆為(37.1±2.9)、(37.6±3.1)和(37.4±3.1)週,差異無統計學意義(F=0.54,P=0.58).CA19/CA19在小于胎齡兒中的頻率明顯高于大于胎齡兒及適于胎齡兒[分彆為25.0％(9/36)、7.6％(7/92)及12.9％(56/433),x2=7.01,P=0.03],但CA19/CAno19及CAno19/CAno19基因型頻率在大于胎齡兒、適于胎齡兒及小于胎齡兒3組之間差異無統計學意義(CA19/CAno19:x2=1.13,P=0.57；CAno19/CAno19:x2=0.58,P=0.75). 結論 IGF-1啟動子區存在基因多態性,CA19等位基因頻率與齣生胎齡無關.
목적 검측신생인이도소양생장인자-1(insulin-like growth factor-1,IGF-1)계동자구역737/738위점포밀정선감(cytosine-adenosine,CA)중복서렬적기인다태성. 방법 선택2010년6월1일지2012년6월30일우북경대학제삼의원출생적건강신생인561례.기록신생인적성별、출생태령、출생체중、신장,병진행태령평고.출생후3～5 d청신공복채집족근혈,제취DNA,분석IGF-1계동자구역기인다태성.채용x2검험、독립양본t검험、방차분석화Hardy-Weinberg평형검험진행통계학분석. 결과 561례신생인중,족월인413례,조산인148례；대우태령인92례,괄우태령인433례,소우태령인36례.561례공발현7충불동적등위기인급23충불동적기인형.7충등위기인분별위188、190、192、194、196、198화200 bp.최상견적3충기인형위190～192 bp、192～196 bp급192～192 bp,기빈솔분별위23.2％(130/561)、15.0％(84/561)화12.8％(72/561).CA19순합기인형(CA19/CA19)、CA19잡합기인형(CA19/CAno19)급CA19돌변기인형(CAno19/CAno19)적빈솔재족월인화조산인지간차이균무통계학의의[분별위11.4％(47/413)여16.9％(25/148)、55.9％(231/413)여50.7％(75/148)、32.7％(135/413)여32.4％(48/148),x2=2.96、1.21화0.00,P균＞0.05].CA19/CA19、CA19/CAno19、CAno19/CAno19저3충기인형신생인적출생태령분별위(37.1±2.9)、(37.6±3.1)화(37.4±3.1)주,차이무통계학의의(F=0.54,P=0.58).CA19/CA19재소우태령인중적빈솔명현고우대우태령인급괄우태령인[분별위25.0％(9/36)、7.6％(7/92)급12.9％(56/433),x2=7.01,P=0.03],단CA19/CAno19급CAno19/CAno19기인형빈솔재대우태령인、괄우태령인급소우태령인3조지간차이무통계학의의(CA19/CAno19:x2=1.13,P=0.57；CAno19/CAno19:x2=0.58,P=0.75). 결론 IGF-1계동자구존재기인다태성,CA19등위기인빈솔여출생태령무관.
Objective To investigate polymorphism in the promoter region of insulin-like growth factor-1(IGF-1) gene.Methods Five hundred and sixty-one neonates admitted to Peking University Third Hospital from June 1st,2010 to June 30th,2012 were recruited into the study.Gender,gestational age,birth weight and birth length were collected.Heel blood samples were collected on 3-5 days after birth.DNA was extracted to analyze the polymorphism in the promoter region of IGF-1 gene.Chi-square test,independent-sample t-test,analysis of variance and HardyWeinberg equilibrium were performed.Results Among the 561 neonates,413 were full term,and 148 were preterm; 92 were large for gestational age (LGA),433 were appropriate for gestional age (AGA),and 36 were small for gestional age (SGA).Seven different alleles and 23 genotypes in the promoter region of IGF-1 gene were identified in the population.The seven alleles were 188,190,192,194,196,198 and 200 bp respectively.The three most common genotypes were 190-192 bp,192-196 bp and 192-192 bp,whose frequencies were 23.2％ (130/561),15.0％ (84/561) and 12.8％(72/561).There were no significant differences of cytosine-adenosine (CA)19/CA19,CA19/CAno19and CAno19/CAno19 genotypes between full term and preterm infants [11.4％ (47/413) vs 16.9％(25/148),55.9％ (231/413) vs 50.7％ (75/148) and 32.7％ (135/413) vs 32.4％ (48/148)respectively,x2=2.96,1.21 and 0.00,all P＞0.05].There was no difference in the gestional age among infants with CA19/CA19,CA19/CAno19 and CAno19/CAno19 genotypes [(37.1±2.9),(37.6±3.1) and (37.4±3.1) weeks respectively,F=0.54,P=0.58].The frequency of CA19/CA19 in SGA neonates was higher than that in LGA and AGA neonates [25.0％ (9/36) vs 7.6％(7/92) and 12.9％ (56/433),x2 =7.01,P=0.03],but there were no differences in the frequency of CA19/CAno19 and CAno19/CAno19 among LGA,AGA and SGA neonates (CA19/CAno19:x2 =1.13,P=0.57; CAno19/CAno19:x2 =0.58,P=0.75).Conclusions Polymorphism exists in the promoter region of IGF-1 gene.The gestational age is not associated with the frequency of CA19 allele.
