中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2013年
10期
589-592
,共4页
刘晓虹%杨杰%邓文喻%罗瑞桦%赵路宁%郑璇儿%杨浩鸣
劉曉虹%楊傑%鄧文喻%囉瑞樺%趙路寧%鄭璇兒%楊浩鳴
류효홍%양걸%산문유%라서화%조로저%정선인%양호명
脐带%间质干细胞%肺炎%通气机,机械%导管相关性感染%支气管肺泡灌洗液%内源性抗菌多肽类物质%β防御素
臍帶%間質榦細胞%肺炎%通氣機,機械%導管相關性感染%支氣管肺泡灌洗液%內源性抗菌多肽類物質%β防禦素
제대%간질간세포%폐염%통기궤,궤계%도관상관성감염%지기관폐포관세액%내원성항균다태류물질%β방어소
Umbilical cord%Mesenchymal stem cells%Pneumonia%Ventilators,mechanical%Catheter-related infections%Bronchoalveolar levage fluid%Cathelicidins%beta-Defensins
目的 探讨人脐带间充质干细胞对呼吸机相关性肺炎(ventilator associated pneumonia,VAP)新生儿支气管肺泡灌洗液中细菌生长的抑制作用及机制.方法 选取2012年6月1日至2012年12月31日在广东省妇幼保健院新生儿重症监护病房住院,支气管肺泡灌洗液培养阳性、需气管插管机械通气并诊断为VAP的患儿为研究对象.每例患儿收集支气管肺泡灌洗液标本2份,随机分入研究组和对照组.研究组中加入人脐带间充质干细胞悬液,对照组中加入等量细胞培养基,共培育6h后采用平板计数法对2组共培育后的细菌进行菌落数(colony-forming unit,CFU)计数.采用酶联免疫吸附法检测内源性抗菌肽(Cathelicidin/LL-37)及人β-防御素-2(human β-defensin-2,HBD-2)的水平,应用Western印迹法检测Cathelicidin/LL-37及HBD-2的特异性蛋白表达.采用配对t检验对菌落数及抗菌肽数据进行统计学分析.结果 31例患儿的支气管肺泡灌洗液中,培养出肺炎克雷伯菌及嗜麦芽窄食单胞菌各6例(各占19.3％),溶血葡萄球菌5例(16.1％),大肠埃希菌、脑膜败血伊丽沙白金菌和鲍曼不动杆菌各3例(各占9.7％),恶臭假单胞菌2例(6.4％),铜绿假单胞菌、阴沟肠杆菌和金黄色葡萄球菌各1例(各占3.2％).研究组菌落数目低于对照组_分别为(2.60±0.67)×104 CFU/ml与(1.18±0.32)×105 CFU/ml,t=-20.19,P＜0.01].研究组共培育液中Cathelicidin/LL-37和HBD-2的水平高于对照组[Cathelicidin/LL-37:(8.98±3.22) ng/ml与(3.18±1.57) ng/ml,t=17.79;HBD-2:(379.87±11.74) pg/ml与(39.89±2.86) pg/ml,t=37.62;P均＜0.01].Western印迹法检测到Cathelicidin/LL-37的特异性蛋白表达,并发现研究组中的Cathelicidin/LL-37表达高于对照组.结论 人脐带间充质干细胞可能通过分泌抗菌肽Cathelicidin/LL-37及HBD-2抑制VAP患者支气管肺泡灌洗液中的细菌生长.
目的 探討人臍帶間充質榦細胞對呼吸機相關性肺炎(ventilator associated pneumonia,VAP)新生兒支氣管肺泡灌洗液中細菌生長的抑製作用及機製.方法 選取2012年6月1日至2012年12月31日在廣東省婦幼保健院新生兒重癥鑑護病房住院,支氣管肺泡灌洗液培養暘性、需氣管插管機械通氣併診斷為VAP的患兒為研究對象.每例患兒收集支氣管肺泡灌洗液標本2份,隨機分入研究組和對照組.研究組中加入人臍帶間充質榦細胞懸液,對照組中加入等量細胞培養基,共培育6h後採用平闆計數法對2組共培育後的細菌進行菌落數(colony-forming unit,CFU)計數.採用酶聯免疫吸附法檢測內源性抗菌肽(Cathelicidin/LL-37)及人β-防禦素-2(human β-defensin-2,HBD-2)的水平,應用Western印跡法檢測Cathelicidin/LL-37及HBD-2的特異性蛋白錶達.採用配對t檢驗對菌落數及抗菌肽數據進行統計學分析.結果 31例患兒的支氣管肺泡灌洗液中,培養齣肺炎剋雷伯菌及嗜麥芽窄食單胞菌各6例(各佔19.3％),溶血葡萄毬菌5例(16.1％),大腸埃希菌、腦膜敗血伊麗沙白金菌和鮑曼不動桿菌各3例(各佔9.7％),噁臭假單胞菌2例(6.4％),銅綠假單胞菌、陰溝腸桿菌和金黃色葡萄毬菌各1例(各佔3.2％).研究組菌落數目低于對照組_分彆為(2.60±0.67)×104 CFU/ml與(1.18±0.32)×105 CFU/ml,t=-20.19,P＜0.01].研究組共培育液中Cathelicidin/LL-37和HBD-2的水平高于對照組[Cathelicidin/LL-37:(8.98±3.22) ng/ml與(3.18±1.57) ng/ml,t=17.79;HBD-2:(379.87±11.74) pg/ml與(39.89±2.86) pg/ml,t=37.62;P均＜0.01].Western印跡法檢測到Cathelicidin/LL-37的特異性蛋白錶達,併髮現研究組中的Cathelicidin/LL-37錶達高于對照組.結論 人臍帶間充質榦細胞可能通過分泌抗菌肽Cathelicidin/LL-37及HBD-2抑製VAP患者支氣管肺泡灌洗液中的細菌生長.
목적 탐토인제대간충질간세포대호흡궤상관성폐염(ventilator associated pneumonia,VAP)신생인지기관폐포관세액중세균생장적억제작용급궤제.방법 선취2012년6월1일지2012년12월31일재광동성부유보건원신생인중증감호병방주원,지기관폐포관세액배양양성、수기관삽관궤계통기병진단위VAP적환인위연구대상.매례환인수집지기관폐포관세액표본2빈,수궤분입연구조화대조조.연구조중가입인제대간충질간세포현액,대조조중가입등량세포배양기,공배육6h후채용평판계수법대2조공배육후적세균진행균락수(colony-forming unit,CFU)계수.채용매련면역흡부법검측내원성항균태(Cathelicidin/LL-37)급인β-방어소-2(human β-defensin-2,HBD-2)적수평,응용Western인적법검측Cathelicidin/LL-37급HBD-2적특이성단백표체.채용배대t검험대균락수급항균태수거진행통계학분석.결과 31례환인적지기관폐포관세액중,배양출폐염극뢰백균급기맥아착식단포균각6례(각점19.3％),용혈포도구균5례(16.1％),대장애희균、뇌막패혈이려사백금균화포만불동간균각3례(각점9.7％),악취가단포균2례(6.4％),동록가단포균、음구장간균화금황색포도구균각1례(각점3.2％).연구조균락수목저우대조조_분별위(2.60±0.67)×104 CFU/ml여(1.18±0.32)×105 CFU/ml,t=-20.19,P＜0.01].연구조공배육액중Cathelicidin/LL-37화HBD-2적수평고우대조조[Cathelicidin/LL-37:(8.98±3.22) ng/ml여(3.18±1.57) ng/ml,t=17.79;HBD-2:(379.87±11.74) pg/ml여(39.89±2.86) pg/ml,t=37.62;P균＜0.01].Western인적법검측도Cathelicidin/LL-37적특이성단백표체,병발현연구조중적Cathelicidin/LL-37표체고우대조조.결론 인제대간충질간세포가능통과분비항균태Cathelicidin/LL-37급HBD-2억제VAP환자지기관폐포관세액중적세균생장.
Objective To explore the effect and its possible mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) on bacterial growth in bronchoalveolar lavage fluid (BALF) of newborns with ventilator associated pneumonia (VAP).Methods Newborns admitted to neonatal intensive care unit (NICU) in Guangdong Women and Children Hospital Affiliated to Guangzhou Medical University from June 1,2012 to December 31,2012 were reviewed.The inclusion criteria were:(1)Positive BALF culture results.(2) Requirement of mechanical ventilation with tracheal intubation.(3) Diagnosed as ventilator-associated pneumonia.Two pieces of BALF samples of newborn were collected and randornaly divided into experimental and control group.hUCMSCs were added into the experimental group,while the same volume of conditioned medium was added into the control group.Both groups were incubated for six hours in humidified CO2 incubator at 37 ℃,then,bacterial growth was assessed by colony forming unit (CFU) counts.Levels of the antimicrobial peptides (Cathelicidin/LL-37 and human HBD-2) were determined by enzyme-linked immunosorbent assay and Western blot.Paired t-test was used for statistical analysis.Results Among the culture results of 31 newborns,there were Klebsiella pneumoniae (6 cases,19.3％),Stenotrophomonas narrow food aeromonas (6 cases,19.3％),Hemolytic staphylococci (5 cases,16.1％),Escherichia coli (3 cases,9.7％),Bacterial meningitis septicemia Elizabeth Platinum (3 cases,9.7％),Acinetobacter baumannii (3 cases,9.7％),Pseudomonas putida (2 cases,6.4％),Pseudomonas aeruginosa (1 case,3.2％),Staphylococcus aureus (1 case,3.2％) and Enterobacter cloacae (1 ease,3.2％).The CFU counts in experimental group were much less than those in control group [(2.60±0.67) ×104] CFU/ml vs [(1.18±0.32) ×105] CFU/ml,(t=-20.19,P＜0.01).Levels of Cathelicidin/LL-37 and HBD-2 in experimental group were higher than those in control group [Cathelicidin/LL-37:(8.98 ± 3.22) ng/ml vs (3.18 ± 1.57) ng/ml,t =17.79,P ＜ 0.01 ;HBD-2:(379.87±11.74) pg/ml vs (39.89±2.86) pg/ml,t=37.62,P＜0.01].Conclusions hUCMSCs had antimicrobial effect on bacterial growth in BALFs from ventilator associated pneumonia possibly by the secretion of antimicrobial peptides (Cathelicidin/LL-37 and HBD-2).
