中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2013年
12期
766-769
,共4页
常红%张秋业%庄超%盛红玲%吴蒙蒙%朱海燕
常紅%張鞦業%莊超%盛紅玲%吳矇矇%硃海燕
상홍%장추업%장초%성홍령%오몽몽%주해연
免疫球蛋白类,静脉内%胎血%T淋巴细胞%婴儿,早产
免疫毬蛋白類,靜脈內%胎血%T淋巴細胞%嬰兒,早產
면역구단백류,정맥내%태혈%T림파세포%영인,조산
Immunoglobulins,intravenous%Fetal blood%T-lymphocytes%Infant,premature
目的 观察不同剂量静脉免疫球蛋白(intravenous immunoglobulin,IVIG)对早产儿脐血T淋巴细胞功能的影响,探讨IVIG免疫调节作用的机制.方法 选择2011年6月1日至2011年12月31日在青岛大学医学院附属医院出生、胎龄32～34周的早产儿15例为研究对象.在无菌条件下采用密度梯度离心法获取脐血单个核细胞(cord blood mononuclear cells,CBMC),置于不同浓度IVIG(A、B、C组分别为6、9和12 mg/ml)条件下体外培养72h.采用流式细胞仪检测CD4+CD25+ Foxp3+调节性T淋巴细胞(regulatery T lymphocyte,Treg细胞)的表达率,酶联免疫吸附法检测培养上清液中干扰素(interferon,IFN)γ、白细胞介素(interleukin,IL)4、IL-10和转化生长因子(transforming growth factor,TGF)-β1的水平.采用单因素方差分析和LSD检验进行统计学处理.结果 (1) CBMC分泌IFN-γ及IL-4水平变化:C组CBMC分泌IFN 7水平为0.03±0.02,低于A组的0.18±0.08和B组的0.13±0.05(F=5.72,P＜0.01),而A组与B组差异无统计学意义(P＞0.05);B组和C组CBMC分泌IL-4水平(分别为0.02±0.01和0.01±0.01)均显著低于A组的0.03±0.01(F=20.38,P均＜0.01),B组与C组差异无统计学意义(P＞0.05);(2)各组Treg细胞的表达率差异均无统计学意义(F=0.67,P均＞0.05).(3)C组CBMC分泌IL-10水平为3.02±3.79,明显低于B组(6.90±4.64)和A组(10.78±5.44)(F=4.68,P＜0.05和0.01);B组也明显低于A组(P＜0.05);B组(16.15±13.94)和C组(8.44±13.71)CBMC分泌TGF-β1水平均显著低于A组(30.23±16.32)(F=5.22,P＜0.05和0.01),B组与C组差异无统计学意义(P＞0.05).结论 IVIG可抑制早产儿脐血辅助性T淋巴细胞1和2的细胞功能,且与其剂量有关;IVIG可通过调节细胞因子IL 10和TGF-β1的分泌抑制天然脐血Treg细胞的功能,且有剂量依赖效应.
目的 觀察不同劑量靜脈免疫毬蛋白(intravenous immunoglobulin,IVIG)對早產兒臍血T淋巴細胞功能的影響,探討IVIG免疫調節作用的機製.方法 選擇2011年6月1日至2011年12月31日在青島大學醫學院附屬醫院齣生、胎齡32～34週的早產兒15例為研究對象.在無菌條件下採用密度梯度離心法穫取臍血單箇覈細胞(cord blood mononuclear cells,CBMC),置于不同濃度IVIG(A、B、C組分彆為6、9和12 mg/ml)條件下體外培養72h.採用流式細胞儀檢測CD4+CD25+ Foxp3+調節性T淋巴細胞(regulatery T lymphocyte,Treg細胞)的錶達率,酶聯免疫吸附法檢測培養上清液中榦擾素(interferon,IFN)γ、白細胞介素(interleukin,IL)4、IL-10和轉化生長因子(transforming growth factor,TGF)-β1的水平.採用單因素方差分析和LSD檢驗進行統計學處理.結果 (1) CBMC分泌IFN-γ及IL-4水平變化:C組CBMC分泌IFN 7水平為0.03±0.02,低于A組的0.18±0.08和B組的0.13±0.05(F=5.72,P＜0.01),而A組與B組差異無統計學意義(P＞0.05);B組和C組CBMC分泌IL-4水平(分彆為0.02±0.01和0.01±0.01)均顯著低于A組的0.03±0.01(F=20.38,P均＜0.01),B組與C組差異無統計學意義(P＞0.05);(2)各組Treg細胞的錶達率差異均無統計學意義(F=0.67,P均＞0.05).(3)C組CBMC分泌IL-10水平為3.02±3.79,明顯低于B組(6.90±4.64)和A組(10.78±5.44)(F=4.68,P＜0.05和0.01);B組也明顯低于A組(P＜0.05);B組(16.15±13.94)和C組(8.44±13.71)CBMC分泌TGF-β1水平均顯著低于A組(30.23±16.32)(F=5.22,P＜0.05和0.01),B組與C組差異無統計學意義(P＞0.05).結論 IVIG可抑製早產兒臍血輔助性T淋巴細胞1和2的細胞功能,且與其劑量有關;IVIG可通過調節細胞因子IL 10和TGF-β1的分泌抑製天然臍血Treg細胞的功能,且有劑量依賴效應.
목적 관찰불동제량정맥면역구단백(intravenous immunoglobulin,IVIG)대조산인제혈T림파세포공능적영향,탐토IVIG면역조절작용적궤제.방법 선택2011년6월1일지2011년12월31일재청도대학의학원부속의원출생、태령32～34주적조산인15례위연구대상.재무균조건하채용밀도제도리심법획취제혈단개핵세포(cord blood mononuclear cells,CBMC),치우불동농도IVIG(A、B、C조분별위6、9화12 mg/ml)조건하체외배양72h.채용류식세포의검측CD4+CD25+ Foxp3+조절성T림파세포(regulatery T lymphocyte,Treg세포)적표체솔,매련면역흡부법검측배양상청액중간우소(interferon,IFN)γ、백세포개소(interleukin,IL)4、IL-10화전화생장인자(transforming growth factor,TGF)-β1적수평.채용단인소방차분석화LSD검험진행통계학처리.결과 (1) CBMC분비IFN-γ급IL-4수평변화:C조CBMC분비IFN 7수평위0.03±0.02,저우A조적0.18±0.08화B조적0.13±0.05(F=5.72,P＜0.01),이A조여B조차이무통계학의의(P＞0.05);B조화C조CBMC분비IL-4수평(분별위0.02±0.01화0.01±0.01)균현저저우A조적0.03±0.01(F=20.38,P균＜0.01),B조여C조차이무통계학의의(P＞0.05);(2)각조Treg세포적표체솔차이균무통계학의의(F=0.67,P균＞0.05).(3)C조CBMC분비IL-10수평위3.02±3.79,명현저우B조(6.90±4.64)화A조(10.78±5.44)(F=4.68,P＜0.05화0.01);B조야명현저우A조(P＜0.05);B조(16.15±13.94)화C조(8.44±13.71)CBMC분비TGF-β1수평균현저저우A조(30.23±16.32)(F=5.22,P＜0.05화0.01),B조여C조차이무통계학의의(P＞0.05).결론 IVIG가억제조산인제혈보조성T림파세포1화2적세포공능,차여기제량유관;IVIG가통과조절세포인자IL 10화TGF-β1적분비억제천연제혈Treg세포적공능,차유제량의뢰효응.
Objective To observe the influence of different doses of intravenous immunoglobulin (IVIG) on function of T cells in cord blood of premature infants and to explore the immunomodulating mechanism of IVIG.Methods Cord blood mononuclear cells (CBMC) were isolated by density gradient centrifugation method from 15 preterm infants born between June 1,2011 to December 31,2011 at 32 34 gestational weeks.Three groups were formed according to the concentrations of IVIG used for CMBC culturing in vitro (Group A,6 mg/ml; Group B,9 mg/ml; Group C,12 mg/ml).After 72 hours in sterile conditions,the levels of IFN 7,IL 4,IL-10 and TGF β1 in the supernatant were determined by enzyme-linked immunosorbent assay.The expression of CD4+ CD25+ Foxp3+ Treg cells was examined by fluorescent activated cell sorter.Differences among groups were compared by one way analysis of variance.For comparison between two groups,LSD test was used.Results (1) The level of IFN γ secreted by CBMC in Group C was 0.03 ± 0.02,which was much lower than that in Group A(0.18±0.08) and Group B(0.13±0.05) (F=5.72,both P＜0.01).The level of IFN-γ in Group B and Group A did not show statistical difference (P＞0.05).Compared with Group A(0.03±0.01),the level of IL4 was much lower in Group B (0.02±0.01) and Group C(0.01±0.01) (F=20.38,both P＜0.01),while no significant difference was shown between Group B and Group C (P＞0.05).(2) No significant difference was found in the expression of CD4+CD25+Foxp3+Treg cells among different groups (F 0.67,P＞0.05).(3) The level of IL 10 in Group C was 3.02 ± 3.79,which was significantly lower than that in Group A (10.78±5.44) and Group B (6.90±4.64)(F=4.68,P＜0.01 and 0.05).The level of IL-10 inGroup B was still lower than that in Group A (P＜0.05).The levels of TGF-β1 in Group C(8.44± 13.71) and Group B(16.15 ±13.94) were significantly lower than that in Group A(30.23 ± 16.32) (F=5.22,P＜0.01,P＜0.05),but there was no significant difference between Group B and Group C (P＞0.05).Conclusions IVIG might inhibit the function of Th cells in CBMC of preterm infants in a dose dependent manner.IVIG could inhibit the function of natural Treg cells by regulating the secretion of cytokines IL-10 and TGF-β1 in a dose dependent effect.
