中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2014年
10期
696-700
,共5页
胎儿缺氧%心室重构%大鼠
胎兒缺氧%心室重構%大鼠
태인결양%심실중구%대서
Fetal hypoxia%Ventricular remodeling%Rats
目的 研究妊娠不同时期缺氧对雄性子代大鼠成年期心肌重构的影响. 方法 将24只Sprague Dawley孕鼠随机分为早期缺氧组、中期缺氧组、晚期缺氧组和空白对照组,每组6只.缺氧组孕鼠分别自妊娠第3、9或1 5天起,每日置于氧气浓度为(10±1)%的低氧舱3h,至自然分娩.各组雄性子代大鼠于3和5月龄时检测血压、左心室质量指数(左心室质量/体重)、心肌纤维直径,以及心肌Ⅰ型和Ⅲ型胶原蛋白表达情况.采用单因素方差分析和SNK检验进行统计学分析. 结果 早期缺氧组子代大鼠3和5月龄收缩压明显升高,与同月龄空白对照组比较,差异有统计学意义[3月龄:(122.0±11.7) mmHg与(108.3±9.5) mmHg,F=2.97;5月龄:(128.5±7.9) mmHg与(114.6±10.0) mmHg,F=3.17;P值均<0.05;1 mmHg=0.133 kPa].3月龄时,中期缺氧组子代大鼠的心脏质量指数和左心室质量指数升高,与空白对照组比较,差异有统计学意义[心脏质量指数:2.92±0.06与2.67±0.06,左心室质量指数:2.15±0.05与1.98±0.02,P值均<0.05].5月龄时,早、中期缺氧组的心脏质量指数、左心室质量指数明显高于空白对照组[心脏质量指数:2.68±0.05和2.64±0.06与2.39±0.30,左心室质量指数:1.99±0.03和1.96±0.05与1.80±0.02],差异有统计学意义(P值均<0.05).3和5月龄雄性子代大鼠心肌组织HE染色切片光镜下见心肌细胞结构清晰,排列规整,各组间心肌纤维直径差异无统计学意义.3月龄时,中期缺氧组与空白对照组比较,心肌组织Ⅰ型和Ⅲ型胶原蛋白相对表达量增加[Ⅰ型胶原蛋白:0.98±0.02与0.87±0.02;Ⅲ型胶原蛋白:0.87±0.01与0.82±0.01;P值均<0.05],中期缺氧组Ⅲ型胶原蛋白相对表达量也较晚期缺氧组(0.79±0.02)增加,差异有统计学意义(P<0.01).5月龄时,早期缺氧组心肌组织Ⅰ型和Ⅲ型胶原蛋白的相对表达量明显高于空白对照组(Ⅰ型胶原:1.76±0.07与0.48±0.04;Ⅲ型胶原:0.68±0.05与0.34±0.03;P值均<0.01),且明显高于晚期缺氧组(Ⅰ型和Ⅲ型胶原分别为0.63±0.07和0.41±0.03),差异有统计学意义(P值均<0.01).中期缺氧组心肌组织Ⅰ型和Ⅲ型胶原蛋白的相对表达量(分别为1.61±0.05和0.61±0.04)明显高于空白对照组和晚期缺氧组,差异有统计学意义(P值均<0.01). 结论 妊娠不同时期缺氧对雄性子代大鼠成年期心脏组织具有不同的影响.妊娠早、中期开始缺氧会引起子代心脏胶原沉积,而不出现心肌细胞肥大,且心脏胶原沉积可能独立于血压改变.
目的 研究妊娠不同時期缺氧對雄性子代大鼠成年期心肌重構的影響. 方法 將24隻Sprague Dawley孕鼠隨機分為早期缺氧組、中期缺氧組、晚期缺氧組和空白對照組,每組6隻.缺氧組孕鼠分彆自妊娠第3、9或1 5天起,每日置于氧氣濃度為(10±1)%的低氧艙3h,至自然分娩.各組雄性子代大鼠于3和5月齡時檢測血壓、左心室質量指數(左心室質量/體重)、心肌纖維直徑,以及心肌Ⅰ型和Ⅲ型膠原蛋白錶達情況.採用單因素方差分析和SNK檢驗進行統計學分析. 結果 早期缺氧組子代大鼠3和5月齡收縮壓明顯升高,與同月齡空白對照組比較,差異有統計學意義[3月齡:(122.0±11.7) mmHg與(108.3±9.5) mmHg,F=2.97;5月齡:(128.5±7.9) mmHg與(114.6±10.0) mmHg,F=3.17;P值均<0.05;1 mmHg=0.133 kPa].3月齡時,中期缺氧組子代大鼠的心髒質量指數和左心室質量指數升高,與空白對照組比較,差異有統計學意義[心髒質量指數:2.92±0.06與2.67±0.06,左心室質量指數:2.15±0.05與1.98±0.02,P值均<0.05].5月齡時,早、中期缺氧組的心髒質量指數、左心室質量指數明顯高于空白對照組[心髒質量指數:2.68±0.05和2.64±0.06與2.39±0.30,左心室質量指數:1.99±0.03和1.96±0.05與1.80±0.02],差異有統計學意義(P值均<0.05).3和5月齡雄性子代大鼠心肌組織HE染色切片光鏡下見心肌細胞結構清晰,排列規整,各組間心肌纖維直徑差異無統計學意義.3月齡時,中期缺氧組與空白對照組比較,心肌組織Ⅰ型和Ⅲ型膠原蛋白相對錶達量增加[Ⅰ型膠原蛋白:0.98±0.02與0.87±0.02;Ⅲ型膠原蛋白:0.87±0.01與0.82±0.01;P值均<0.05],中期缺氧組Ⅲ型膠原蛋白相對錶達量也較晚期缺氧組(0.79±0.02)增加,差異有統計學意義(P<0.01).5月齡時,早期缺氧組心肌組織Ⅰ型和Ⅲ型膠原蛋白的相對錶達量明顯高于空白對照組(Ⅰ型膠原:1.76±0.07與0.48±0.04;Ⅲ型膠原:0.68±0.05與0.34±0.03;P值均<0.01),且明顯高于晚期缺氧組(Ⅰ型和Ⅲ型膠原分彆為0.63±0.07和0.41±0.03),差異有統計學意義(P值均<0.01).中期缺氧組心肌組織Ⅰ型和Ⅲ型膠原蛋白的相對錶達量(分彆為1.61±0.05和0.61±0.04)明顯高于空白對照組和晚期缺氧組,差異有統計學意義(P值均<0.01). 結論 妊娠不同時期缺氧對雄性子代大鼠成年期心髒組織具有不同的影響.妊娠早、中期開始缺氧會引起子代心髒膠原沉積,而不齣現心肌細胞肥大,且心髒膠原沉積可能獨立于血壓改變.
목적 연구임신불동시기결양대웅성자대대서성년기심기중구적영향. 방법 장24지Sprague Dawley잉서수궤분위조기결양조、중기결양조、만기결양조화공백대조조,매조6지.결양조잉서분별자임신제3、9혹1 5천기,매일치우양기농도위(10±1)%적저양창3h,지자연분면.각조웅성자대대서우3화5월령시검측혈압、좌심실질량지수(좌심실질량/체중)、심기섬유직경,이급심기Ⅰ형화Ⅲ형효원단백표체정황.채용단인소방차분석화SNK검험진행통계학분석. 결과 조기결양조자대대서3화5월령수축압명현승고,여동월령공백대조조비교,차이유통계학의의[3월령:(122.0±11.7) mmHg여(108.3±9.5) mmHg,F=2.97;5월령:(128.5±7.9) mmHg여(114.6±10.0) mmHg,F=3.17;P치균<0.05;1 mmHg=0.133 kPa].3월령시,중기결양조자대대서적심장질량지수화좌심실질량지수승고,여공백대조조비교,차이유통계학의의[심장질량지수:2.92±0.06여2.67±0.06,좌심실질량지수:2.15±0.05여1.98±0.02,P치균<0.05].5월령시,조、중기결양조적심장질량지수、좌심실질량지수명현고우공백대조조[심장질량지수:2.68±0.05화2.64±0.06여2.39±0.30,좌심실질량지수:1.99±0.03화1.96±0.05여1.80±0.02],차이유통계학의의(P치균<0.05).3화5월령웅성자대대서심기조직HE염색절편광경하견심기세포결구청석,배렬규정,각조간심기섬유직경차이무통계학의의.3월령시,중기결양조여공백대조조비교,심기조직Ⅰ형화Ⅲ형효원단백상대표체량증가[Ⅰ형효원단백:0.98±0.02여0.87±0.02;Ⅲ형효원단백:0.87±0.01여0.82±0.01;P치균<0.05],중기결양조Ⅲ형효원단백상대표체량야교만기결양조(0.79±0.02)증가,차이유통계학의의(P<0.01).5월령시,조기결양조심기조직Ⅰ형화Ⅲ형효원단백적상대표체량명현고우공백대조조(Ⅰ형효원:1.76±0.07여0.48±0.04;Ⅲ형효원:0.68±0.05여0.34±0.03;P치균<0.01),차명현고우만기결양조(Ⅰ형화Ⅲ형효원분별위0.63±0.07화0.41±0.03),차이유통계학의의(P치균<0.01).중기결양조심기조직Ⅰ형화Ⅲ형효원단백적상대표체량(분별위1.61±0.05화0.61±0.04)명현고우공백대조조화만기결양조,차이유통계학의의(P치균<0.01). 결론 임신불동시기결양대웅성자대대서성년기심장조직구유불동적영향.임신조、중기개시결양회인기자대심장효원침적,이불출현심기세포비대,차심장효원침적가능독립우혈압개변.
Objective To evaluate whether the timing of maternal hypoxia during pregnancy could myocardial remodeling in adult offspring.Methods Twenty-four pregnant rats were assigned to the maternal hypoxia group starting from the early period of pregnancy (G1,day 3 to 21 of pregnancy),the group starting from the middle period of pregnancy (G2,day 9 to 21 of pregnancy),the group starting from the late period of pregnancy (G3,day 15 to 21 of pregnancy),or the control group (G0).Six rats were included in each group.Rats in the maternal hypoxia groups (G1,G2 and G3) were subjected to hypoxia for 3 hours in a low pressure cabin with an oxygen concentration of (10± 1) %.Systolic blood pressure,wet weight of the left ventricle normalized for body weight (left ventricular weight/body weight,LVW/BW),diameter of cardiomyocytes,and the expression levels of collagen Ⅰ and collagen Ⅲ were measured in adult male offspring at the age of 3 and 5 months.One-way ANOVA and SNK test were used for statistical analysis.Results Maternal hypoxia from day 3 to 21 of pregnancy induced higher systolic blood pressure in male offspring at the age of 3 months and 5 months [3 months:G1 (122.0± 11.7) mmHg,G0 (108.3±9.5) mmHg,F=2.97; 5 months:G1 (128.5±7.9) mmHg,G0 (114.6±-10.0) mmHg,F=3.17,both P<0.05].Maternal hypoxia also led to a higher LVW/BW ratio (G1 1.99±0.03,G0 1.80±0.02,P<0.05),and increased collagen Ⅰ and collagen Ⅲ expression (collagen Ⅰ /β-actin:G1 1.76±0.07,G0 0.48±0.04,P<0.01; collagen Ⅲ /β-actin:G1 0.68±0.05,G0 0.34±0.03,P<0.01) in adult male offspring at the age of 5 months.Maternal hypoxia from day 9 to 21 of pregnancy induced a higher LVW/BW ratio (3 months:G2 2.15 ± 0.05,G0 1.98 ± 0.02,P<0.05; 5 months:G2 1.96±±0.05,G0 1.80±0.02,P<0.05),and increased collagen Ⅰ and collagen Ⅲ expression (3 months:collagen Ⅰ /β-actin G2 0.98±±0.02,G0 0.87±0.02,P<0.05; collagen Ⅲ /β-actin:G2 0.87±±0.01,G0 0.82±0.01,P<0.05; 5 months; collagen Ⅰ /β-actin G2 1.61±0.05,G0 0.48±0.04,P<0.01; collagen Ⅲ / β-actin:G2 0.61 ±0.04,G0 0.34±0.03,P<0.01) in male offspring at the age of 3 months and 5 months,but did not affect systolic blood pressure.Maternal hypoxia from day 15 to 2l did not have any effect.The diameter of cardiomyocytes in male offspring was not significantly different between the groups.Conclusions Maternal hypoxia during different periods of pregnancy has different effects on cardiac structure in adult rat offspring.Maternal hypoxia started in early or mid-pregnancy leads to cardiac collagen deposition without an increase in myocyte size in adult offspring,which may be independent of the change in blood pressure.