中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2012年
11期
1015-1020
,共6页
王轩%张宏伟%张安玲%韩磊%王坤%浦佩玉%申长虹%康春生%于春江
王軒%張宏偉%張安玲%韓磊%王坤%浦珮玉%申長虹%康春生%于春江
왕헌%장굉위%장안령%한뢰%왕곤%포패옥%신장홍%강춘생%우춘강
脑干肿瘤%神经胶质瘤%微RNAs%基因表达谱%微阵列分析
腦榦腫瘤%神經膠質瘤%微RNAs%基因錶達譜%微陣列分析
뇌간종류%신경효질류%미RNAs%기인표체보%미진렬분석
Brain stem neoplasms%Glioma%Micro RNAs%Gene expression profiling%Microarray analysis
目的 筛选儿童型脑干胶质瘤(BSG)对照于成人型BSG差异表达的miRNA,为探索儿童型BSG发病机制和治疗中的miRNA干预策略提供可供选择的目标miRNA.方法 利用miRNA 芯片检测儿童型与成人型BSG原位动物模型的miRNA表达谱,分析二者差异性表达的miRNA,并挑选差异表达最明显的miRNA在两种BSG的人体标本中进行qRT-PCR和原位杂交验证.结果 两个组别中共检测到216个miRNA表达,以成人型BSG为比照,儿童型BSG中3.5倍以上差异表达上调的miRNA 10个、下调的29个.其中差异最明显的为miR-20a和miR-106b,选取二者在两种BSG的人体标本中进行qRT-PCR和原位杂交验证,结果提示芯片结果适用于人类.结论 研究两种BSG的差异性表达的miRNA,将会为解释儿童型BSG较之成人型BSG的恶性进展及以此为控制BSG这一严重威胁儿童生命的疾病提供新的思路.
目的 篩選兒童型腦榦膠質瘤(BSG)對照于成人型BSG差異錶達的miRNA,為探索兒童型BSG髮病機製和治療中的miRNA榦預策略提供可供選擇的目標miRNA.方法 利用miRNA 芯片檢測兒童型與成人型BSG原位動物模型的miRNA錶達譜,分析二者差異性錶達的miRNA,併挑選差異錶達最明顯的miRNA在兩種BSG的人體標本中進行qRT-PCR和原位雜交驗證.結果 兩箇組彆中共檢測到216箇miRNA錶達,以成人型BSG為比照,兒童型BSG中3.5倍以上差異錶達上調的miRNA 10箇、下調的29箇.其中差異最明顯的為miR-20a和miR-106b,選取二者在兩種BSG的人體標本中進行qRT-PCR和原位雜交驗證,結果提示芯片結果適用于人類.結論 研究兩種BSG的差異性錶達的miRNA,將會為解釋兒童型BSG較之成人型BSG的噁性進展及以此為控製BSG這一嚴重威脅兒童生命的疾病提供新的思路.
목적 사선인동형뇌간효질류(BSG)대조우성인형BSG차이표체적miRNA,위탐색인동형BSG발병궤제화치료중적miRNA간예책략제공가공선택적목표miRNA.방법 이용miRNA 심편검측인동형여성인형BSG원위동물모형적miRNA표체보,분석이자차이성표체적miRNA,병도선차이표체최명현적miRNA재량충BSG적인체표본중진행qRT-PCR화원위잡교험증.결과 량개조별중공검측도216개miRNA표체,이성인형BSG위비조,인동형BSG중3.5배이상차이표체상조적miRNA 10개、하조적29개.기중차이최명현적위miR-20a화miR-106b,선취이자재량충BSG적인체표본중진행qRT-PCR화원위잡교험증,결과제시심편결과괄용우인류.결론 연구량충BSG적차이성표체적miRNA,장회위해석인동형BSG교지성인형BSG적악성진전급이차위공제BSG저일엄중위협인동생명적질병제공신적사로.
Objectives To study the different expression of miRNA between pediatric and adult types of brainstem gliomas,and to provide the target miRNAs for explore the mechanism and miRNA interference of the malignant progression of pediatric BSG.Methods miRNA expression profiles in orthotopic models which could simulate the BSG heterogeneity were examined by microarray and analyzed to obtain the aberrantly expressed miRNAs.The two types of human BSG tissue were utilized to verify the microarray data by qRT-PCR and in situ hybridization for the putative causative miRNAs.Results There were 216 miRNAs detected in both the pediatric BSG group and the adult BSG group,39 miRNAs to be differential expressed in the pediatric BSG group versus adult group,including 10 up-regulated and 29 downregulated.qRT-PCR and in situ hybridization indicated good consistency with that of the microarray method.Conclusions Aberrantly expressed miRNA may serve as putative causative involvement of malignant progression of pediatric BSG,thereby might be potentially novel targets for therapy.
