CAJ | 학술논문

目的检测γ突触核蛋白(SNCG)与基质金属蛋白酶9(MMP-9)在浸润性乳腺癌组织以及T47D和T47 DSNCG-细胞中的表达,探讨SNCG与MMP-9的相关性.方法选择2009年6月至2012年6月手术切除的96例浸润性乳腺癌标本[女性,年龄(56±8)岁],采用免疫组化法分别检测组织标本中SNCG和MMP-9的表达情况.建立T47D与SNCG干扰T47DSNCG-细胞株,采用Western blot法检测SNCG与MMP-9蛋白的表达,real-time PCR检测SNCG与MMP-9基因的表达.结果 96例标本中,26例(27.1％) SNCG与MMP-9同时表达,30例(31.12％)同时不表达,二者之间的表达呈明显正相关(r =0.655,P=0.000).T47D细胞株SNCG蛋白表达水平是T47DSNCG-细胞株的2.1倍,SNCG mRNA表达水平约为T47DSNCG-细胞的13.5倍.T47D细胞株MMP-9蛋白表达水平是T47DSNCG-细胞株的1.6倍,MMP-9 mRNA表达水平是T47DSNCG-细胞株的7.3倍.结论 SNCG、MMP-9蛋白在乳腺癌中的表达呈明显正相关.SNCG可能通过调节MMP-9的表达而影响乳腺癌的浸润、转移.
목적 검측γ돌촉핵단백(SNCG)여기질금속단백매9(MMP-9)재침윤성유선암조직이급T47D화T47 DSNCG-세포중적표체,탐토SNCG여MMP-9적상관성.방법 선택2009년6월지2012년6월수술절제적96례침윤성유선암표본[녀성,년령(56±8)세],채용면역조화법분별검측조직표본중SNCG화MMP-9적표체정황.건립T47D여SNCG간우T47DSNCG-세포주,채용Western blot법검측SNCG여MMP-9단백적표체,real-time PCR검측SNCG여MMP-9기인적표체.결과 96례표본중,26례(27.1％) SNCG여MMP-9동시표체,30례(31.12％)동시불표체,이자지간적표체정명현정상관(r =0.655,P=0.000).T47D세포주SNCG단백표체수평시T47DSNCG-세포주적2.1배,SNCG mRNA표체수평약위T47DSNCG-세포적13.5배.T47D세포주MMP-9단백표체수평시T47DSNCG-세포주적1.6배,MMP-9 mRNA표체수평시T47DSNCG-세포주적7.3배.결론 SNCG、MMP-9단백재유선암중적표체정명현정상관.SNCG가능통과조절MMP-9적표체이영향유선암적침윤、전이.
Objectives To evaluate the expression of synuclein-γ (SNCG) and metalloproteinase 9 (MMP-9) both in the invasive ductal breast cancer samples and T47D and T47DSNCG-cell lines,to investigate the correlation between SNCG and MMP-9.Methods Totally 96 invasive ductal breast cancer samples (female,mean age of (56 ± 8) years) were collected between June 2009 and June 2012.The expressions of SNCG and MMP-9 were investigated by immunohistochemistry.T47D and SNCG knock down T47DSNCG-cell lines were established and SNCG and MMP-9 protein expression were investigated by Western blot and gene expression by real-time PCR.Results Among 96 samples,26 (27.1％) of them co-expressed SNCG and MMP-9,30 (31.2％) of them expressed neither SNCG nor MMP-9.The expression of SNCG was correlated with the expression of MMP-9(r =0.655,P =0.000).SNCG mRNA level of T47D cell line was 13.5 fold of T47DSNCG-cell line and SNCG protein expression was 2.1 fold.While MMP-9 mRNA level of T47D cell line was 7.3 fold of T47DSNCG-cell line and MMP-9 protien expression was 1.6 fold.When SNCG was knocked down,the expression of MMP-9 decreased.Conclusions SNCG and MMP-9 are significantly correlated with each other in breast cancer.SNCG may promote the invasion and metastasis of breast cancer mediated by up-regulating the expression of MMP-9.