中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2014年
8期
612-616
,共5页
邹雷%罗开元%乔鸥%许建彪
鄒雷%囉開元%喬鷗%許建彪
추뢰%라개원%교구%허건표
胃肿瘤%体外研究%近距离放射疗法%碘放射性同位素%基因表达
胃腫瘤%體外研究%近距離放射療法%碘放射性同位素%基因錶達
위종류%체외연구%근거리방사요법%전방사성동위소%기인표체
Stomach neoplasms%In vitro%Brachytherapy%Iodine radioisotopes%Gene expression
目的 探讨胃癌细胞接受碘-125 (125I)粒子照射后的基因表达变化.方法 用125I粒子分别照射高分化(BGC-823)、中分化(AGS)以及低分化(NCI-N87)人胃癌细胞株.每个细胞株分为实验组(接受100 cGy125I粒子照射)和对照组(不接受照射).应用微阵列芯片检测3株细胞株被照射后全基因表达变化情况,设定检验水准(P＜0.05及表达倍数变化大于2)以筛选差异表达的基因.应用实时定量(qRT)-PCR验证差异基因表达情况,应用BisoGenet重建蛋白-蛋白相互作用网络,用Cytoscape复杂网络分析和形象化平台进行基因网络关系分析.应用DAVID进行GO及KEGG通路分析.结果 高、中、低3个不同分化细胞株经相同剂量率的125I粒子照射后,基因芯片的层次聚类分析结果显示,3株胃癌细胞株的对照组和实验组从细胞聚类分析上看是属于同一聚类,NCI-N87细胞经125I粒子照射后,有895个基因表达上调,786个基因表达下调;AGS细胞经125I粒子照射后,有124个基因表达上调,161个基因表达下调;BGC-823细胞经125I粒子照射后,有2 412个基因表达上调,3 243个基因表达下调.电离辐射后会引起非常复杂的转录调控变化,KEGG通路分析说明这些差异表达基因在特定的细胞通路中有重叠.经qRT-PCR验证其中具有相似动力学特性的差异表达基因TRAF3IP2-AS1、SDC1、RABL2B和NOM1 4个基因,与对照组相比均出现了差异表达(P＜0.05).结论 125I粒子照射胃癌细胞株后会导致较多基因表达上调或下调,其中TRAF3IP2-AS1、SDC1、RABL2B和NOM1在3株细胞株均发生显著变化,推测可能是125I粒子治疗胃癌的作用点,为将来研制治疗胃癌靶向药物提供参考.
目的 探討胃癌細胞接受碘-125 (125I)粒子照射後的基因錶達變化.方法 用125I粒子分彆照射高分化(BGC-823)、中分化(AGS)以及低分化(NCI-N87)人胃癌細胞株.每箇細胞株分為實驗組(接受100 cGy125I粒子照射)和對照組(不接受照射).應用微陣列芯片檢測3株細胞株被照射後全基因錶達變化情況,設定檢驗水準(P＜0.05及錶達倍數變化大于2)以篩選差異錶達的基因.應用實時定量(qRT)-PCR驗證差異基因錶達情況,應用BisoGenet重建蛋白-蛋白相互作用網絡,用Cytoscape複雜網絡分析和形象化平檯進行基因網絡關繫分析.應用DAVID進行GO及KEGG通路分析.結果 高、中、低3箇不同分化細胞株經相同劑量率的125I粒子照射後,基因芯片的層次聚類分析結果顯示,3株胃癌細胞株的對照組和實驗組從細胞聚類分析上看是屬于同一聚類,NCI-N87細胞經125I粒子照射後,有895箇基因錶達上調,786箇基因錶達下調;AGS細胞經125I粒子照射後,有124箇基因錶達上調,161箇基因錶達下調;BGC-823細胞經125I粒子照射後,有2 412箇基因錶達上調,3 243箇基因錶達下調.電離輻射後會引起非常複雜的轉錄調控變化,KEGG通路分析說明這些差異錶達基因在特定的細胞通路中有重疊.經qRT-PCR驗證其中具有相似動力學特性的差異錶達基因TRAF3IP2-AS1、SDC1、RABL2B和NOM1 4箇基因,與對照組相比均齣現瞭差異錶達(P＜0.05).結論 125I粒子照射胃癌細胞株後會導緻較多基因錶達上調或下調,其中TRAF3IP2-AS1、SDC1、RABL2B和NOM1在3株細胞株均髮生顯著變化,推測可能是125I粒子治療胃癌的作用點,為將來研製治療胃癌靶嚮藥物提供參攷.
목적 탐토위암세포접수전-125 (125I)입자조사후적기인표체변화.방법 용125I입자분별조사고분화(BGC-823)、중분화(AGS)이급저분화(NCI-N87)인위암세포주.매개세포주분위실험조(접수100 cGy125I입자조사)화대조조(불접수조사).응용미진렬심편검측3주세포주피조사후전기인표체변화정황,설정검험수준(P＜0.05급표체배수변화대우2)이사선차이표체적기인.응용실시정량(qRT)-PCR험증차이기인표체정황,응용BisoGenet중건단백-단백상호작용망락,용Cytoscape복잡망락분석화형상화평태진행기인망락관계분석.응용DAVID진행GO급KEGG통로분석.결과 고、중、저3개불동분화세포주경상동제량솔적125I입자조사후,기인심편적층차취류분석결과현시,3주위암세포주적대조조화실험조종세포취류분석상간시속우동일취류,NCI-N87세포경125I입자조사후,유895개기인표체상조,786개기인표체하조;AGS세포경125I입자조사후,유124개기인표체상조,161개기인표체하조;BGC-823세포경125I입자조사후,유2 412개기인표체상조,3 243개기인표체하조.전리복사후회인기비상복잡적전록조공변화,KEGG통로분석설명저사차이표체기인재특정적세포통로중유중첩.경qRT-PCR험증기중구유상사동역학특성적차이표체기인TRAF3IP2-AS1、SDC1、RABL2B화NOM1 4개기인,여대조조상비균출현료차이표체(P＜0.05).결론 125I입자조사위암세포주후회도치교다기인표체상조혹하조,기중TRAF3IP2-AS1、SDC1、RABL2B화NOM1재3주세포주균발생현저변화,추측가능시125I입자치료위암적작용점,위장래연제치료위암파향약물제공삼고.
Objective To study genome-wide gene expression changes in gastric cancer cells after iodine-125 (125 I) particle irradiation.Methods 125I particles were used to irradiate three gastric cancer cell lines of various differentiation levels:high (BGC-823),medium (AGS) and low (NCI-N87).Whole-genome gene expression was investigated with microarray.The gene expression in iodine-125 irradiated and untreated cancer cells was compared,and the genes with transcript levels altered for at least 2 folds (P ＜ 0.05) were selected.The change in gene expression levels was verified by using quantitative real-time (qRT)-PCR.Results The three gastric cancer cell lines received the same dose rate of 125I particle irradiation.Cluster analysis showed that the Gene Ontology(GO) categories did not change in the three cell lines,but changes in gene expression levels were evident for many genes.After 125I particle irradiate NCI-N87 cells,895 genes were up-regulated,786 genes were down-regulated; AGS was irradiated by 125I seed,there were 124 genes upregulated,161 genes were down-regulated; BGC-823 cells were treated by 125I seed irradiation,2 412 genes upregulated,3 243 downregulated genes.After ionizing radiation can cause very complex transcriptional regulation changes,KEGG pathway analysis shows that these differentially expressed genes overlap in a particular cell pathway.Four genes,TRAF3IP2-AS1,SDC1,RABL2B and NOM,were found having at least 2-fold difference in expression (P ＜ 0.05),and the gene expression alteration was confirmed by qRT-PCR.Conclusions 125I particle irradiation caused gene expression changes in gastric cancer cells.The expressions of TRAF3IP2-AS1,SDC1,RABL2B and NOM are altered significantly in all three cell lines studied,indicating that these genes may play an important role in the 125I seed treatment of gastric cancer.These genes could be potential targets for developing anti-cancer drugs in the future.
