CAJ | 학술논문

目的探讨射频消融(RFA)治疗大鼠肝肿瘤对其脾脏组织内树突状细胞(DC)表型的影响及其意义.方法选取40只正常Sprague Dawley大鼠,10只作为正常对照组,其余30只制作Walker-256肝肿瘤模型后,随机分为RFA后7 d组、RFA后14 d组和荷瘤对照组,每组10只.RFA后7 d组、RFA后14 d组分别于RFA处理后7 d及14 d处死,荷瘤对照组与正常对照组不做RFA处理即处死.取其脾脏组织,采用Ficoll密度梯度离心法分离出单个核细胞,应用流式细胞术检测单个核细胞OX62、OX6及CD86表达水平.结果正常大鼠与荷瘤大鼠的脾脏单个核细胞中分别有(10.36±3.21)%、(11.69±4.39)%表达OX62,二者比较差异无统计学意义(P＞0.05).正常大鼠与荷瘤大鼠的脾脏单个核细胞中分别有(76.33±7.86)%、(60.58±6.26)%表达OX6;而分别有(63.06±8.77)%、(40.87±8.66)%表达CD86,2者比较差异均有统计学意义(P＜0.05).RFA后7 d 0X62阳性表达率为(10.24±2.49)%,RFA后14 d为(15.10±2.37)%,RFA后14 d组与荷瘤对照组比较差异有统计学意义(P＜0.05).RFA后7 d OX6阳性表达率为(77.45±4.53)%,RFA后14 d为(75.47±5.22)%,2组与荷瘤对照组比较差异均有统计学意义(P＜0.05).RFA后7 d CD86阳性表达率为(46.86±9.42)%,RFA后14 d为(45.53±9.13)%,2组与荷瘤对照组比较差异均无统计学意义(P＞0.05).结论荷瘤鼠脾脏组织中未成熟DC比例高,抗原提呈能力弱,RFA促使荷瘤大鼠外周血中DC前体细胞迁移到脾脏组织中的数量增多,并进一步分化成熟,对提高机体在免疫应答中的抗原提呈能力起到促进作用.
목적 탐토사빈소융(RFA)치료대서간종류대기비장조직내수돌상세포(DC)표형적영향급기의의.방법 선취40지정상Sprague Dawley대서,10지작위정상대조조,기여30지제작Walker-256간종류모형후,수궤분위RFA후7 d조、RFA후14 d조화하류대조조,매조10지.RFA후7 d조、RFA후14 d조분별우RFA처리후7 d급14 d처사,하류대조조여정상대조조불주RFA처리즉처사.취기비장조직,채용Ficoll밀도제도리심법분리출단개핵세포,응용류식세포술검측단개핵세포OX62、OX6급CD86표체수평.결과 정상대서여하류대서적비장단개핵세포중분별유(10.36±3.21)%、(11.69±4.39)%표체OX62,이자비교차이무통계학의의(P＞0.05).정상대서여하류대서적비장단개핵세포중분별유(76.33±7.86)%、(60.58±6.26)%표체OX6;이분별유(63.06±8.77)%、(40.87±8.66)%표체CD86,2자비교차이균유통계학의의(P＜0.05).RFA후7 d 0X62양성표체솔위(10.24±2.49)%,RFA후14 d위(15.10±2.37)%,RFA후14 d조여하류대조조비교차이유통계학의의(P＜0.05).RFA후7 d OX6양성표체솔위(77.45±4.53)%,RFA후14 d위(75.47±5.22)%,2조여하류대조조비교차이균유통계학의의(P＜0.05).RFA후7 d CD86양성표체솔위(46.86±9.42)%,RFA후14 d위(45.53±9.13)%,2조여하류대조조비교차이균무통계학의의(P＞0.05).결론 하류서비장조직중미성숙DC비례고,항원제정능력약,RFA촉사하류대서외주혈중DC전체세포천이도비장조직중적수량증다,병진일보분화성숙,대제고궤체재면역응답중적항원제정능력기도촉진작용.
Objective To study the influence of radio-frequency ablation (RFA) of liver tumors on the phenotype of spleen dendritic cells in rats.MethodsTen healthy Sprague Dawley rats constituted the normal control group.Thirty experimental Walker-256 liver tumor model rats were prepared by implantating tumor particles,and these were then randomly divided equally into three groups:a 7 days after RFA group,a 14 d after RFA group and a tumor control group. The spleen tissue of all groups was sampled before the experiment,and the spleens of the 7 d and 14 d after RFA groups were sampled again 7 d and 14 d after RFA respectively.The mononuclear cells in the spleen tissue were separated through Ficoll density gradient centrifugation.The expression level of OX62,OX6 and CD86 in the mononuelear spleen cells was analyzed with flow eytometry. ResultsIn the spleens of the normal control rats (10.36±3.21) % of mononuclear cells expressed OX-62.In the tumor control group the proportion was (11.69±4.39) % ,and the difference between the two groups was not significant.In the spleens of the normal rats (76.33±7.86)% of mononuelear cells expressed OX-6,while in the minor control group the ratio was (60.58±6.26)% ,a significant difference.In the spleens of the normal rats (63.06±8.77) % of mononuclear cells expressed CD-86,while in the tumor control rats the ratio was (40.87±8.66) % ,a difference which was not significant.In spleens of rats with tumors 7 d and 14 d after RFA the proportion of OX62 was (10.24±2.49)% and (15.10±2.37)% respectively.The difference between the tumor control group and the 14 d after RFA group was significant.The proportion of OX-6 in the 7 d and 14 d groups was (77.45±4.53) % and (75.47±5.22) % respectively,and both levels were significantly different from the tumor control group.The proportion of CD86 was (46.86±9.42) % and (45.53±9.13) % in the 7 d and 14 d RFA groups respectively,and neither was significantly different from the tumor control group. Conclusions Immature dendritic cells were more common in the spleens of rats with tumors than in the normal controls.Their antigen-presenting capacity was weaker.RFA can increase the number of precursor dendritic cells migrating from peripheral blood to the spleen,and those cells may further differentiate or mature,which may improve antigen-presenting capacity to a certain extent.