CAJ | 학술논문

目的探讨高压氧(HBO)联合细胞周期特异性药物氟尿嘧啶对结肠腺癌Lovo细胞的杀伤作用.方法 0.20 MPa HBO暴露后联合应用不同浓度氟尿嘧啶处理结肠腺癌Lovo细胞.流式细胞仪检测细胞周期,四甲基偶氮唑盐(MTT)法观察其对结肠腺癌细胞增殖的抑制作用.结果 ①HBO暴露后Lovo细胞S期细胞积聚较对照组明显增多(P＜0.01),HBO暴露后24 h组Lovo细胞S期积聚明显高于HBO暴露后12 h组和48 h组(P＜0.01)；②0.20 MPa HBO暴露后24 h联合应用低浓度氟尿嘧啶(≤8 μM)作用Lovo细胞12 h后,HBO联合氟尿嘧啶组癌细胞增殖的抑制程度与氟尿嘧啶组比较,差异有统计学意义(P＜0.05)；药物作用48 h后,HBO联合氟尿嘧啶组与氟尿嘧啶组比较,差异有统计学意义(P＜0.01)；③中浓度和高浓度(16、32和64 μM)的氟尿嘧啶作用于Lovo细胞12 h,HBO联合氟尿嘧啶组与氟尿嘧啶组之间差异无统计学意义(JP＞0.05)；药物作用48 h后,HBO联合氟尿嘧啶组与氟尿嘧啶组比较差异有统计学意义(P＜0.05).结论 0.20 MPa HBO暴露可提高细胞周期特异性药物氟尿嘧啶对Lovo细胞的杀伤作用,尤其增强低浓度氟尿嘧啶对结肠腺癌细胞的杀伤作用.
목적 탐토고압양(HBO)연합세포주기특이성약물불뇨밀정대결장선암Lovo세포적살상작용.방법 0.20 MPa HBO폭로후연합응용불동농도불뇨밀정처리결장선암Lovo세포.류식세포의검측세포주기,사갑기우담서염(MTT)법관찰기대결장선암세포증식적억제작용.결과 ①HBO폭로후Lovo세포S기세포적취교대조조명현증다(P＜0.01),HBO폭로후24 h조Lovo세포S기적취명현고우HBO폭로후12 h조화48 h조(P＜0.01)；②0.20 MPa HBO폭로후24 h연합응용저농도불뇨밀정(≤8 μM)작용Lovo세포12 h후,HBO연합불뇨밀정조암세포증식적억제정도여불뇨밀정조비교,차이유통계학의의(P＜0.05)；약물작용48 h후,HBO연합불뇨밀정조여불뇨밀정조비교,차이유통계학의의(P＜0.01)；③중농도화고농도(16、32화64 μM)적불뇨밀정작용우Lovo세포12 h,HBO연합불뇨밀정조여불뇨밀정조지간차이무통계학의의(JP＞0.05)；약물작용48 h후,HBO연합불뇨밀정조여불뇨밀정조비교차이유통계학의의(P＜0.05).결론 0.20 MPa HBO폭로가제고세포주기특이성약물불뇨밀정대Lovo세포적살상작용,우기증강저농도불뇨밀정대결장선암세포적살상작용.
Objective To investigate the effect of hyperbaric oxygen(HBO)combined with 5-fluorouracil (5-FU)chemotherapy on colon carcinoma Lovo cells.Methods Lovo ceils were exposed to 0.20 MPa HBO combined with 5-FU at different concentrations.The cell cycle was monitored with flow cytometry,and cell proliferation was detected using a methylthiazol tetrazolium test(MTT).Results ①After exposure to HBO,phase accumulation of Lovo cells was significantly higher than in the control group,and the accumulation of Lovo cells exposed to HBO after 24 h was significantly higher than at 12 and 48 hours.②There was difference between HBO(0.20 MPa,post 24 h)+ 5-FU(≤8 pM)group and 5-FU(≤8 μM,12 h)group on the inhibition of cells proliferation(P ＜0.05),and significan difference could be seen between the two groups after treatment for 48 h(P ＜ 0.01); ③There was no significant difference between HBO + 5-FU group and 5-FU group treatment with the concentration(16,32,64 μM)of 5-FU(P ＞ 0.05)for 12 h ;however,the difference could be seen after treatment for 48 h between the two groups(P ＜ 0.05).Conclusion The lethal effect on Lovo cells of 5-FU can be enhanced by exposure to 0.20 MPa HBO,especially with low concentrations of 5-FU.