CAJ | 학술논문

目的观察重复经颅磁刺激(rTMS)对急性脑缺血大鼠室管膜下区(SVZ)神经干细胞增殖和微小核糖核酸-9(miR-9)表达的影响,探讨rTMS治疗急性脑缺血的可能机制.方法将雄性SD大鼠按随机数字表法分为假手术组、对照组和rTMS组,采用短暂性大脑中动脉阻塞(tMCAO)法制作急性脑缺血大鼠模型.假手术组和对照组没有进行任何治疗；rTMS组从术后1d开始给予患侧大脑磁刺激治疗(10 Hz,120％静息运动阈值,单序列30个脉冲,间隔50 s,共10个序列),每日1次,共治疗7次.治疗结束后,使用免疫组织荧光化学法检测患侧SVZ的溴脱氧尿苷(BrdU)/巢蛋白(nestin)双阳性细胞以分析神经干细胞增殖情况,并通过实时定量聚合酶链式反应(qRT-PCR)检测患侧脑组织miR-9表达量.结果治疗7d后,对照组患侧SVZ BrdU+ nestin+细胞数较假手术组增高(P＜0.05),rTMS组较其它两组显著增高(P＜0.05).同时,对照组患侧脑组织内miR-9表达显著低于假手术组(P＜0.05),rTMS组miR-9表达均显著低于其它2组(P＜0.05).结论 rTMS治疗可促进急性脑缺血大鼠SVZ神经干细胞增殖,且抑制患侧脑组织miR-9表达.
목적 관찰중복경로자자격(rTMS)대급성뇌결혈대서실관막하구(SVZ)신경간세포증식화미소핵당핵산-9(miR-9)표체적영향,탐토rTMS치료급성뇌결혈적가능궤제.방법 장웅성SD대서안수궤수자표법분위가수술조、대조조화rTMS조,채용단잠성대뇌중동맥조새(tMCAO)법제작급성뇌결혈대서모형.가수술조화대조조몰유진행임하치료；rTMS조종술후1d개시급여환측대뇌자자격치료(10 Hz,120％정식운동역치,단서렬30개맥충,간격50 s,공10개서렬),매일1차,공치료7차.치료결속후,사용면역조직형광화학법검측환측SVZ적추탈양뇨감(BrdU)/소단백(nestin)쌍양성세포이분석신경간세포증식정황,병통과실시정량취합매련식반응(qRT-PCR)검측환측뇌조직miR-9표체량.결과 치료7d후,대조조환측SVZ BrdU+ nestin+세포수교가수술조증고(P＜0.05),rTMS조교기타량조현저증고(P＜0.05).동시,대조조환측뇌조직내miR-9표체현저저우가수술조(P＜0.05),rTMS조miR-9표체균현저저우기타2조(P＜0.05).결론 rTMS치료가촉진급성뇌결혈대서SVZ신경간세포증식,차억제환측뇌조직miR-9표체.
Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) on the proliferation of neural stem cells in the subventricular zone (SVZ) and the expression of miR-9 in the ipsilesional hemisphere of rats with focal cerebral ischemia.Methods A rat model of acute focal cerebral ischemia was established using transient middle cerebral artery occlusion (tMCAO).Rats were then randomly assigned to a sham group,a control group or an rTMS group.Daily rTMS treatments (10 trains with 30 pulses per train and 50-second breaks between trains at 120％ of the resting motor threshold) were targeted at the ipsilesional MI cortex beginning one day after the tMCAO.After the treatment the proliferation of neural stem cells in the ipsilesional SVZ was identified by double immunofluorescence staining (BrdU/nestin).The expression of miR-9 was evaluated using quantitative PCR.Results Compared with the other two groups,BrdU + Nestin + cells in the ipsilesional SVZs of the rTMS group increased and the expression of miR-9 decreased significantly.Conclusion rTMS can promote the proliferation of neural stem cells and inhibit the expression of miR-9 in the ipsilesional hemisphere after focal cerebral ischemia.