CAJ | 학술논문

目的通过检测神经干细胞β-连接素基因和蛋白的表达,初步探讨脉冲强磁场(0.1 Hz,4T,8次)促神经干细胞增殖的作用机制.结果取新生SD大鼠双侧脑室下区神经干细胞,无血清培养2周,将细胞分成实验组和对照组.实验组的细胞给予0.1 Hz 4 T的脉冲强磁场下预8次；对照组的细胞置相同条件下不行干预.此后第1、3、5和7天,分别用RT-PCR和Western blot检测两组神经干细胞β-连接素的表达.结果干预后第1、3、5和7天,RT-PCR法显示实验组神经干细胞β连接素基因表达较对照组明显增高(P＜ 0.05)；Western blot也显示实验组神经干细胞β-连接素蛋白表达较对照组明显增高,增高水平在干预后第7天最显著.结论新生大鼠神经干细胞经0.1 Hz,4T脉冲强磁场干预8次后,β-连接素在基因及蛋白水平均增高,提示脉冲强磁场(0.1 Hz,4T,8次)促神经干细胞增殖的机制可能是通过激活Wnt/β-连接素通路实现.
목적 통과검측신경간세포β-련접소기인화단백적표체,초보탐토맥충강자장(0.1 Hz,4T,8차)촉신경간세포증식적작용궤제.결과 취신생SD대서쌍측뇌실하구신경간세포,무혈청배양2주,장세포분성실험조화대조조.실험조적세포급여0.1 Hz 4 T적맥충강자장하예8차；대조조적세포치상동조건하불행간예.차후제1、3、5화7천,분별용RT-PCR화Western blot검측량조신경간세포β-련접소적표체.결과 간예후제1、3、5화7천,RT-PCR법현시실험조신경간세포β련접소기인표체교대조조명현증고(P＜ 0.05)；Western blot야현시실험조신경간세포β-련접소단백표체교대조조명현증고,증고수평재간예후제7천최현저.결론 신생대서신경간세포경0.1 Hz,4T맥충강자장간예8차후,β-련접소재기인급단백수평균증고,제시맥충강자장(0.1 Hz,4T,8차)촉신경간세포증식적궤제가능시통과격활Wnt/β-련접소통로실현.
Objective To study the mechanism by which a high-intensity pulsed electromagnetic field (HIPEMF) (0.1 Hz,4 T,8 pulses) facilitates the proliferation of neural stem cells by detecting the expression of β-catenin genes and protein.Methods Neural stem cells (NSCs) were isolated from the sub-ventricular zone (SVZ) of neonatal rats and cultured in supplemented,serum-free medium for two weeks.The NSCs were then divided into an experimental group exposed to a HIPEMF for 8 pulses and a control group given sham stimulation.The gene and prorein expression of β-catenin in the NSCs were assayed by RT-PCR and Western blotting on the 1st,3rd,5th and 7th day after the stimulation.Results The NSCs' cloned spheres were round and translucent,and showed red fluorescence after staining with anti-nestin (cy3).The RT-PCR results showed β-catenin genes were highly expressed in the exposed group (significantly more than in the controls).The Western blotting showed that expression of β-catenin protein was also higher in the experimental group,especially at the 7th day after stimulation,a difference which was also statistically significant Conclusion HIPEMF at 0.1 Hz,4 T,in 8 pulse trains can promote NSC proliferation,perhaps through the Wnt/β-catenin signaling pathways.