中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
12期
889-893
,共5页
王全懂%顾平%董巧云%王彦永%崔冬生%王铭维
王全懂%顧平%董巧雲%王彥永%崔鼕生%王銘維
왕전동%고평%동교운%왕언영%최동생%왕명유
经颅磁刺激%黑质%纹状体%帕金森病
經顱磁刺激%黑質%紋狀體%帕金森病
경로자자격%흑질%문상체%파금삼병
Transcranial magnetic stimulation%Substantia nigra%Corpus striatum%Parkinson's disease
目的 观察重复经颅磁刺激(rTMS)对帕金森病(PD)模型鼠黑质纹状体系统的保护作用.方法 40只雄性C57BL/6J小鼠按随机数字表法分为对照组、模型组、假磁刺激组和磁刺激组,每组10只.小鼠皮下注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP),按15 mg/kg体重每2h注射1次,注射4次,每次间隔2h,制作帕金森病模型；用重复经颅磁刺激方法进行干预,利用免疫组化染色技术检测黑质酪氨酸羟化酶(TH)阳性神经元数目及纹状体TH阳性产物的表达变化,并借助图像分析系统对其进行定量分析；利用尼氏染色观察黑质多巴胺神经元尼氏小体(Nissl body)的表达变化；利用嗜银染色技术显示纹状体区神经纤维的形态学变化.结果 对照组、模型组、假磁刺激组和磁刺激组黑质区TH阳性神经元平均数目分别为(55.20±11.60)、(23.60 ±9.90)、(21.30±8.40)和(39.20±12.40),磁刺激组较对照组的明显减少(P＜0.05),但明显高于模型组和假磁刺激组(P＜0.05)；模型组和假磁刺激组比较,差异无统计学意义(P＞0.05).对照组黑质区多巴胺神经元中布满着深蓝色呈颗粒状的尼氏体,模型组和假磁刺激组多巴胺神经元内尼氏体大量脱失,存在的尼氏体着色浅淡,磁刺激组多巴胺神经元内尼氏体有一定程度的脱失,但明显减轻.磁刺激组纹状体区TH阳性纤维校正光密度(COD)值为(0.278 ±0.014)较对照组的(0.304 ±0.012)明显减少(P＜0.05)；模型组和假磁刺激组相应的COD值分别为(0.247±0.014)和(0.243±0.030),磁刺激组分别与模型组和假磁刺激组比较,差异均有统计学意义(P＜0.05)；模型组与假磁刺激组之间比较,差异无统计学意义(P＞0.05).对照组纹状体区可见大量呈束状、密集、排列规则有序的神经纤维；模型组和假磁刺激组纹状体区神经纤维稀疏、断裂且排列零散无序；磁刺激组与模型组和假磁刺激组纹状体区染色结果相比,仍可见呈束状较为密集,且排列较有序的神经纤维.结论 磁刺激可有效保护受损的黑质区TH阳性神经元及纹状体区TH阳性纤维,从而推测磁刺激可能通过保护多巴胺神经元及其投射纤维,从而增加多巴胺合成与转运达到治疗作用.
目的 觀察重複經顱磁刺激(rTMS)對帕金森病(PD)模型鼠黑質紋狀體繫統的保護作用.方法 40隻雄性C57BL/6J小鼠按隨機數字錶法分為對照組、模型組、假磁刺激組和磁刺激組,每組10隻.小鼠皮下註射1-甲基-4-苯基-1,2,3,6-四氫吡啶(MPTP),按15 mg/kg體重每2h註射1次,註射4次,每次間隔2h,製作帕金森病模型；用重複經顱磁刺激方法進行榦預,利用免疫組化染色技術檢測黑質酪氨痠羥化酶(TH)暘性神經元數目及紋狀體TH暘性產物的錶達變化,併藉助圖像分析繫統對其進行定量分析；利用尼氏染色觀察黑質多巴胺神經元尼氏小體(Nissl body)的錶達變化；利用嗜銀染色技術顯示紋狀體區神經纖維的形態學變化.結果 對照組、模型組、假磁刺激組和磁刺激組黑質區TH暘性神經元平均數目分彆為(55.20±11.60)、(23.60 ±9.90)、(21.30±8.40)和(39.20±12.40),磁刺激組較對照組的明顯減少(P＜0.05),但明顯高于模型組和假磁刺激組(P＜0.05)；模型組和假磁刺激組比較,差異無統計學意義(P＞0.05).對照組黑質區多巴胺神經元中佈滿著深藍色呈顆粒狀的尼氏體,模型組和假磁刺激組多巴胺神經元內尼氏體大量脫失,存在的尼氏體著色淺淡,磁刺激組多巴胺神經元內尼氏體有一定程度的脫失,但明顯減輕.磁刺激組紋狀體區TH暘性纖維校正光密度(COD)值為(0.278 ±0.014)較對照組的(0.304 ±0.012)明顯減少(P＜0.05)；模型組和假磁刺激組相應的COD值分彆為(0.247±0.014)和(0.243±0.030),磁刺激組分彆與模型組和假磁刺激組比較,差異均有統計學意義(P＜0.05)；模型組與假磁刺激組之間比較,差異無統計學意義(P＞0.05).對照組紋狀體區可見大量呈束狀、密集、排列規則有序的神經纖維；模型組和假磁刺激組紋狀體區神經纖維稀疏、斷裂且排列零散無序；磁刺激組與模型組和假磁刺激組紋狀體區染色結果相比,仍可見呈束狀較為密集,且排列較有序的神經纖維.結論 磁刺激可有效保護受損的黑質區TH暘性神經元及紋狀體區TH暘性纖維,從而推測磁刺激可能通過保護多巴胺神經元及其投射纖維,從而增加多巴胺閤成與轉運達到治療作用.
목적 관찰중복경로자자격(rTMS)대파금삼병(PD)모형서흑질문상체계통적보호작용.방법 40지웅성C57BL/6J소서안수궤수자표법분위대조조、모형조、가자자격조화자자격조,매조10지.소서피하주사1-갑기-4-분기-1,2,3,6-사경필정(MPTP),안15 mg/kg체중매2h주사1차,주사4차,매차간격2h,제작파금삼병모형；용중복경로자자격방법진행간예,이용면역조화염색기술검측흑질락안산간화매(TH)양성신경원수목급문상체TH양성산물적표체변화,병차조도상분석계통대기진행정량분석；이용니씨염색관찰흑질다파알신경원니씨소체(Nissl body)적표체변화；이용기은염색기술현시문상체구신경섬유적형태학변화.결과 대조조、모형조、가자자격조화자자격조흑질구TH양성신경원평균수목분별위(55.20±11.60)、(23.60 ±9.90)、(21.30±8.40)화(39.20±12.40),자자격조교대조조적명현감소(P＜0.05),단명현고우모형조화가자자격조(P＜0.05)；모형조화가자자격조비교,차이무통계학의의(P＞0.05).대조조흑질구다파알신경원중포만착심람색정과립상적니씨체,모형조화가자자격조다파알신경원내니씨체대량탈실,존재적니씨체착색천담,자자격조다파알신경원내니씨체유일정정도적탈실,단명현감경.자자격조문상체구TH양성섬유교정광밀도(COD)치위(0.278 ±0.014)교대조조적(0.304 ±0.012)명현감소(P＜0.05)；모형조화가자자격조상응적COD치분별위(0.247±0.014)화(0.243±0.030),자자격조분별여모형조화가자자격조비교,차이균유통계학의의(P＜0.05)；모형조여가자자격조지간비교,차이무통계학의의(P＞0.05).대조조문상체구가견대량정속상、밀집、배렬규칙유서적신경섬유；모형조화가자자격조문상체구신경섬유희소、단렬차배렬령산무서；자자격조여모형조화가자자격조문상체구염색결과상비,잉가견정속상교위밀집,차배렬교유서적신경섬유.결론 자자격가유효보호수손적흑질구TH양성신경원급문상체구TH양성섬유,종이추측자자격가능통과보호다파알신경원급기투사섬유,종이증가다파알합성여전운체도치료작용.
Objective To investigate the effect of repetitive transcranial magnetic stimulation (rTMS) on dopaminergic neurons in substantia nigra and nigrostriate fibers in the striatum in cases of Parkinson's disease (PD).Methods Forty male C57BL/6J mice were randomly divided into a control group,a PD model group,a sham-rTMS (srTMS) group and an rTMS group with 10 mice in each group.A PD model was established by subcutaneous injection of a solution of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP),and the mice were treated with rTMS or sham rTMS for 14 days.Tyrosine hydroxylase (TH) expression in the substantia and striatum were detected using an immunohistochemical technique,and the corrected optical densities (CODs) of TH in the striatum were analysed using an image analysis system.The Nissl bodies were detected by Nissl staining.The morphological disposition of nerve fibers in the striatum was detected using Warthin-Starry staining.Results The fraction of neurons expressing TH decreased significantly more in the substantia nigras of mice in the rTMS group than in the control group,the PD group and the srTMS group.In the control group the neural plasm of dopaminergic neurons was full of dark blue and granular Nissl bodies.Many Nissl bodies were lost in the PD and srTMS groups,and the remaining Nissl bodies were colored lightly.Few Nissl bodies were lost in the rTMS group.Positive TH reactions in the striatum were significantly decreased in the rTMS group compared to the control group.The average COD was also significantly lower.But positive TH reactions in the striata of the rTMS group mice were significantly greater than in the PD and srTMS groups,and the average COD was significantly higher.In the control group,the disposition of nerve fibers in the striatum was typically fasciculated,concentrated and ordered; in PD and srTMS groups,many fibers were lost,and the remaining nerve fibers were rare,ruptured and scattered ; in the rTMS group fewer nerve fibers were lost and the disposition of the remaining fibers was more fasciculated,concentrated and ordered than in the PD and srTMS groups.Conclusion rTMS may play a role in treating Parkinson's disease by protecting dopaminergic neurons as well as nigrostriate fibers and by improving the synthesis and transport of dopamine.
