CAJ | 학술논문

目的观察红光照射对大鼠缺氧缺血性脑损伤(HIBD)急性期凋亡因子Caspase-8及Caspase-3表达的影响.方法采用随机数字表法将45只SD大鼠分为假手术组、缺氧缺血性脑损伤组(简称模型组)及红光照射组.采用经典Rice法将模型组及红光照射组大鼠制成HIBD模型,假手术组仅分离左颈总动脉后缝合.红光照射组于HIBD损伤后立即对其额顶部进行红光照射,连续照射3d.于制模后第3天时取各组大鼠损伤侧海马组织,采用实时PCR技术检测Caspase-8及Caspase-3 mRNA表达,采用Western blot 技术检测Caspase-8及Caspase-3蛋白表达；采用免疫荧光法观察各组大鼠Caspase-8及Caspase-3定位并进行半定量分析.结果制模后第3天时发现模型组和红光照射组Caspase-8、Caspase-3基因及蛋白表达均较假手术组明显增强(P＜0.05)；红光照射组Caspase-8基因表达(1.55 ±0.12)及蛋白表达(0.70 ±0.18)、Caspase-3基因表达[(1.48±0.16)×10-4]及蛋白表达(0.74 ±0.13)均较模型组显著降低(P＜0.05).结论红光照射可通过抑制细胞凋亡因子Caspase-8、Caspase-3表达,减轻HIBD急性期神经细胞凋亡,从而发挥HIBD治疗作用.
목적 관찰홍광조사대대서결양결혈성뇌손상(HIBD)급성기조망인자Caspase-8급Caspase-3표체적영향.방법 채용수궤수자표법장45지SD대서분위가수술조、결양결혈성뇌손상조(간칭모형조)급홍광조사조.채용경전Rice법장모형조급홍광조사조대서제성HIBD모형,가수술조부분리좌경총동맥후봉합.홍광조사조우HIBD손상후립즉대기액정부진행홍광조사,련속조사3d.우제모후제3천시취각조대서손상측해마조직,채용실시PCR기술검측Caspase-8급Caspase-3 mRNA표체,채용Western blot 기술검측Caspase-8급Caspase-3단백표체；채용면역형광법관찰각조대서Caspase-8급Caspase-3정위병진행반정량분석.결과 제모후제3천시발현모형조화홍광조사조Caspase-8、Caspase-3기인급단백표체균교가수술조명현증강(P＜0.05)；홍광조사조Caspase-8기인표체(1.55 ±0.12)급단백표체(0.70 ±0.18)、Caspase-3기인표체[(1.48±0.16)×10-4]급단백표체(0.74 ±0.13)균교모형조현저강저(P＜0.05).결론 홍광조사가통과억제세포조망인자Caspase-8、Caspase-3표체,감경HIBD급성기신경세포조망,종이발휘HIBD치료작용.
Objective To investigate the effect of red light on the expression of caspase-8 and caspase-3 mRNA and protein during the acute stage of hypoxic-ischemic brain damage (HIBD) in neonatal rats.Methods Forty-five seven-day-old Sprague-Dawley rats were randomly divided into a sham group,an HIBD model group (model group) and an irradiation group.The rats in the model and irradiation groups were subject to HIBD induced using the Rice-Vannucci method.The irradiation group was treated by irradiation with red light on the forehead immediately after the establishment of the HIBD model for 30 min/d on 3 consecutive days,while the sham group and the model group received no treatment.On the 3rd day after the operation,10 rats from each group were sacrificed by cervical dislocation and the left hippocampus was rapidly isolated and snap-frozen in liquid nitrogen for mRNA and protein examination.Another 5 rats in each group were used for immunofluorescence testing to localize and semi-quantify the expression of caspase-8 and caspase-3.Results In the model group,expression of caspase-8 and caspase-3 mRNA and protein in the left hippocampus was higher than in the sham group on the 3rd day.After red light irradiation,expression of caspase-8 and caspase-3 mRNA and protein decreased significantly in the irradiation group compared with the HIBD group.In the CA 1 region of the hippocampus,the levels of caspase-8 and caspase-3 in the irradiation group were significantly lower than in the model group.Conclusion Red light irradiation can decrease the expression of such apoptosis factors as caspase-8 and caspase-3 in hypoxic-ischemic brain damage at the acute stage and inhibit neural cell apoptosis so as to exert therapeutic effects for hypoxic-ischemic brain damage.