CAJ | 학술논문

目的探讨高压氧干预对丙戊酸钠(VPA)孤独症模型鼠学习记忆能力及海马锥体细胞形态学的影响.方法通过Wistar大鼠怀孕第12.5天腹腔注射VPA的方法制作VPA孤独症大鼠模型,孤独症模型大鼠分为高压氧治疗组、高压空气治疗组、常压高氧治疗组、常压空气模型组,每组雄鼠12只；从正常对照组中随机选取12只雄鼠,作为常压空气正常组.孤独症模型鼠的干预措施:采用动物实验舱,高压氧治疗组每次治疗前用纯氧洗舱10 min,纯氧加压15 min,于2.0 ATA稳压45 min,减压15 min;高压空气治疗组空气加压15 min,于2.0 ATA稳压45 min,减压15 min；常压高氧治疗组治疗前纯氧洗舱10 min,不关紧舱门,持续供给纯氧1 h；常压空气模型组每日置于开放的动物实验舱1h,不加压,不关舱门；各组每日干预1次,每次1h,疗程7天.常压空气正常组置于笼内,未做特殊处理.采用Y型电迷宫检测大鼠学习记忆能力,HE染色观察海马锥体细胞的变化.结果高压氧治疗组模型鼠治疗后学习记忆能力提高,表现为Y型电迷宫检测尝试次数减少(30.69±0.63 vs31.54±0.88,t=5.500,P=0.001),再现次数增加(3.38 ±0.65 vs2.85±0.69,t=-2.214,P=0.047);常压高氧治疗组治疗后大鼠学习记忆能力提高,表现为Y型电迷宫检测尝试次数减少(31.15±0.99 vs 31.54±0.97,t=2.739,P=0.018),再现次数增加(3.00±0.58 vs 2.69±0.48,t=-2.309,P=0.040).高压氧干预后海马锥体细胞凋亡减少,正常形态细胞数增多.结论高压氧干预可提高孤独症模型鼠的学习记忆能力；高压氧干预后锥体细胞增殖,凋亡减少,可能是高压氧治疗孤独症发挥作用的途径之一.
목적 탐토고압양간예대병무산납(VPA)고독증모형서학습기억능력급해마추체세포형태학적영향.방법 통과Wistar대서부잉제12.5천복강주사VPA적방법제작VPA고독증대서모형,고독증모형대서분위고압양치료조、고압공기치료조、상압고양치료조、상압공기모형조,매조웅서12지；종정상대조조중수궤선취12지웅서,작위상압공기정상조.고독증모형서적간예조시:채용동물실험창,고압양치료조매차치료전용순양세창10 min,순양가압15 min,우2.0 ATA은압45 min,감압15 min;고압공기치료조공기가압15 min,우2.0 ATA은압45 min,감압15 min；상압고양치료조치료전순양세창10 min,불관긴창문,지속공급순양1 h；상압공기모형조매일치우개방적동물실험창1h,불가압,불관창문；각조매일간예1차,매차1h,료정7천.상압공기정상조치우롱내,미주특수처리.채용Y형전미궁검측대서학습기억능력,HE염색관찰해마추체세포적변화.결과 고압양치료조모형서치료후학습기억능력제고,표현위Y형전미궁검측상시차수감소(30.69±0.63 vs31.54±0.88,t=5.500,P=0.001),재현차수증가(3.38 ±0.65 vs2.85±0.69,t=-2.214,P=0.047);상압고양치료조치료후대서학습기억능력제고,표현위Y형전미궁검측상시차수감소(31.15±0.99 vs 31.54±0.97,t=2.739,P=0.018),재현차수증가(3.00±0.58 vs 2.69±0.48,t=-2.309,P=0.040).고압양간예후해마추체세포조망감소,정상형태세포수증다.결론 고압양간예가제고고독증모형서적학습기억능력；고압양간예후추체세포증식,조망감소,가능시고압양치료고독증발휘작용적도경지일.
Objective To investigate the effects of hyperbaric oxygen on learning and memory ability in rats with a model of VPA autism and on the pyramidal cells in the CA1 region of the hippocampus.Methods An animal model of autism was established in the offspring of Wistar rats which had received a single intraperitoneal injection of sodium valproate at the 12.5th day of pregnancy.A total of 48 male VPA autism model rats were randomly divided into the hyperbaric oxygen group,a high pressure air group,a normal pressure high oxygen group and a normal pressure air group (each group with 12 rats).A normal control group was obtained by injecting physiological saline.The autism model rats of the hyperbaric oxygen group were treated with high pressure oxygen in an animal experiment cabin which was cleaned with pure oxygen for 10 min,pressurized for 15 min,held at 2.0 atmospheres absolute (ATA) for 45 min and then had the pressure relieved over 15 min.For the high pressure air group the cabin was pressurized with air for 15 min,held at 2.0 ATA for 45 min and the pressure was relieved over 15 min.For the normal pressure,high oxygen group the cabin was cleaned for 10 min with pure oxygen,then pure oxygen was supplied for 1 hour with the cabin door open.The normal pressure air group rats were placed in the open cabin with no pressure or additional oxygen.The testing lasted 7 days for 1 hour per day.The normal control group rats were placed in ordinary cages.Learning and memory were evaluated using the Y electric maze test before and after the interventions.Any changes in the pyramidal cells in the CA1 region of the hippocampus were observed after hematoxylin and eosin (HE) staining.Results The average number of tries of the hyperbaric oxygen group after treatment was significantly less than before treatment and memory retention times were increased.The number was also less in the normobaric hyperoxia group and memory retention times again improved.The number of apoptotic cells was reduced and the number of normal form cells in the CA1 region increased after the hyperbaric oxygen intervention.Conclusions The learning and memory abilities of the autistic rats improved after the hyperbaric oxygen intervention.Pyramidal cells in the CA1 region proliferated and the number of apoptotic cell decreased.This may be the mechanism by which hyperbaric oxygen intervention treats autism.