CAJ | 학술논문

目的观察缺血前预运动对大鼠前额叶皮质内抗坏血酸水平及学习记忆能力的影响.方法选取健康3月龄雄性SD大鼠24只,按随机数字表法将其分为运动缺血组、运动对照组、缺血对照组和假手术组,每组6只.运动缺血组和运动对照组大鼠进行跑台训练,共3周,运动缺血组在此基础上采用双侧颈总动脉结扎(2-VO)制备慢性脑缺血大鼠模型,缺血对照组和假手术组大鼠不进行跑台运动训练,常规饲养3周后,缺血对照组建立慢性脑缺血大鼠模型,假手术组仅暴露双侧颈总动脉,不行结扎处理.脑缺血前、后采用活体动物脑内微透析采样及在线电化学分析技术对大鼠的抗坏血酸水平进行测定,脑缺血后24 h应用被动回避反射评价大鼠的学习记忆能力.结果脑缺血前,运动缺血组、运动对照组、缺血对照组及假手术组大鼠前额叶皮质的抗坏血酸基础浓度比较,差异无统计学意义(P＞0.05);运动缺血组大鼠的抗坏血酸浓度在缺血后5 min开始上升,最高值为基础浓度的(203.69±46.48)％,与缺血前比较,差异有统计学意义(P＜0.05);运动对照组和假手术组的抗坏血酸浓度与治疗前比较,差异均无统计学意义(P＞0.05);缺血对照组大鼠的抗坏血酸浓度在脑缺血后5 min内上升至基础浓度的(222.37±42.37)％ (P ＜0.05),且在缺血后lh内上升至(316.98±32.46)％,与缺血前基础浓度比较,差异有统计学意义(P＜0.05).组间比较发现,运动缺血组缺血后各时间点的抗坏血酸浓度均低于缺血对照组(P＜0.05).脑缺血后24 h,运动缺血组大鼠的被动回避反射时间长于缺血对照组,提示运动缺血组大鼠的学习记忆能力优于缺血对照组(P＜0.05).结论脑缺血前预运动可提高大鼠前额叶皮质的抗坏血酸水平,促进其学习记忆能力恢复.
목적 관찰결혈전예운동대대서전액협피질내항배혈산수평급학습기억능력적영향.방법 선취건강3월령웅성SD대서24지,안수궤수자표법장기분위운동결혈조、운동대조조、결혈대조조화가수술조,매조6지.운동결혈조화운동대조조대서진행포태훈련,공3주,운동결혈조재차기출상채용쌍측경총동맥결찰(2-VO)제비만성뇌결혈대서모형,결혈대조조화가수술조대서불진행포태운동훈련,상규사양3주후,결혈대조조건립만성뇌결혈대서모형,가수술조부폭로쌍측경총동맥,불행결찰처리.뇌결혈전、후채용활체동물뇌내미투석채양급재선전화학분석기술대대서적항배혈산수평진행측정,뇌결혈후24 h응용피동회피반사평개대서적학습기억능력.결과 뇌결혈전,운동결혈조、운동대조조、결혈대조조급가수술조대서전액협피질적항배혈산기출농도비교,차이무통계학의의(P＞0.05);운동결혈조대서적항배혈산농도재결혈후5 min개시상승,최고치위기출농도적(203.69±46.48)％,여결혈전비교,차이유통계학의의(P＜0.05);운동대조조화가수술조적항배혈산농도여치료전비교,차이균무통계학의의(P＞0.05);결혈대조조대서적항배혈산농도재뇌결혈후5 min내상승지기출농도적(222.37±42.37)％ (P ＜0.05),차재결혈후lh내상승지(316.98±32.46)％,여결혈전기출농도비교,차이유통계학의의(P＜0.05).조간비교발현,운동결혈조결혈후각시간점적항배혈산농도균저우결혈대조조(P＜0.05).뇌결혈후24 h,운동결혈조대서적피동회피반사시간장우결혈대조조,제시운동결혈조대서적학습기억능력우우결혈대조조(P＜0.05).결론 뇌결혈전예운동가제고대서전액협피질적항배혈산수평,촉진기학습기억능력회복.
Objective To investigate the effect of pre-ischemia physical activity on cognition and ascorbate content in medial prefrontal cortex (mPFC) after cerebral ischemia.Methods Twenty-four Sprague-Dowley rats were enrolled in this study and randomly divided into the following 4 groups:running-ischemia group,running group,ischemia group and shame operation group.Cerebral ischemia was brought about by permanent 2 vessels occlusion (2-VO) method.Treadmill running was used as physical activity training.Ascorbate in mPFC was monitored with in vivo microdialysis coupled with on-line electrochemical flow cell analysis.Passive avoidance was used to test cognitive function at 24 hours after 2-VO cerebral ischemia.Results Neurochemistry study showed that ascorbate level in mPFC increased within 3 hours after 2-VO ischemia and the increase was attenuated in the running-ischemia group.The baseline level of mPFC ascorbate in the four groups has no significant difference.Behavioral data indicated that 3 weeks pre-ischemia running promoted cognitive function recovery after 2-VO ischemia.Conclusion The pre-ischemia physical activity could increase the ascrobate storage in mPFC and enhance the antioxidant ability of this region.Therefore,it is one of the possible neurochemical mechanism underlying pre-ischemia physical activity for the improvement of cognitive function after cerebral ischemia.Thus pre-ischemia physical activity can be of benefit to cognition rehabilitation after stroke.