CAJ | 학술논문

目的研究针刺对快速老化小鼠P8(SAMP8)海马神经元突触可塑性及谷氨酸α-氨基-3-羟基-5-甲基-4-异唑丙酸(AMPA)受体表达的影响.方法选取雄性9月龄SAMP8 19只,按随机数字表法分为模型组(10只)和针刺组(9只),另选取雄性同月龄抗快速老化小鼠(SAMR1)9只作为对照组.针刺组给予“百会”、“涌泉”穴位针刺治疗,每日1次,7d为1个疗程,疗程之间相隔2d,共治疗4个疗程.第4疗程内每次治疗后,采用Morris水迷宫实验评估动物的学习记忆能力.应用透射电镜观察治疗后小鼠海马CA1区神经元突触的超微结构变化,用Western blot法检测小鼠海马AMPA受体亚基谷氨酸受体l(GluR1)及谷氨酸受体2(GluR2)的含量.结果定位航行实验中,与对照组比较,模型组逃避潜伏期延长(P＜0.05),针刺组较模型组缩短(P＜0.05).空间探索实验中,对照组、模型组、针刺组小鼠穿越有效区的次数分别为(5.33±2.29)次、(2.30 ±0.82)次、(5.22±2.05)次,与对照组比较,模型组穿越有效区的次数减少(P＜0.05),针刺组较模型组增加(P＜0.05).与对照组比较,模型组小鼠海马CA1区神经元PSD[(39.83 ±9.30)nm]变薄(P＜0.05)、突触间隙[(19.98±5.21)nm]增宽(P＜0.05)、突触界面曲率(1.12±0.05)下降(P＜0.05)；与模型组比较,针刺组小鼠突触PSD[(46.78±11.37)nm]增厚(P＜0.05)、突触间隙[(15.68±4.03)nm]缩小(P＜0.05)、突触界面曲率(1.14 ±0.09)增大(P＜0.05).模型组小鼠海马GluR1含量较对照组下降(P＞0.05),针刺组较模型组GluR1含量增加(P＞0.05),模型组GluR2含量较对照组下降(P＜0.05),针刺组较模型组GluR2含量增加(P＜0.05).结论针刺可显著提高SAMP8的学习记忆能力及突触可塑性,并促进其海马AMPA受体GluR2亚基表达,提示针刺可能是促进神经康复、改善学习记忆能力的方法之一.
목적 연구침자대쾌속노화소서P8(SAMP8)해마신경원돌촉가소성급곡안산α-안기-3-간기-5-갑기-4-이서병산(AMPA)수체표체적영향.방법 선취웅성9월령SAMP8 19지,안수궤수자표법분위모형조(10지)화침자조(9지),령선취웅성동월령항쾌속노화소서(SAMR1)9지작위대조조.침자조급여“백회”、“용천”혈위침자치료,매일1차,7d위1개료정,료정지간상격2d,공치료4개료정.제4료정내매차치료후,채용Morris수미궁실험평고동물적학습기억능력.응용투사전경관찰치료후소서해마CA1구신경원돌촉적초미결구변화,용Western blot법검측소서해마AMPA수체아기곡안산수체l(GluR1)급곡안산수체2(GluR2)적함량.결과 정위항행실험중,여대조조비교,모형조도피잠복기연장(P＜0.05),침자조교모형조축단(P＜0.05).공간탐색실험중,대조조、모형조、침자조소서천월유효구적차수분별위(5.33±2.29)차、(2.30 ±0.82)차、(5.22±2.05)차,여대조조비교,모형조천월유효구적차수감소(P＜0.05),침자조교모형조증가(P＜0.05).여대조조비교,모형조소서해마CA1구신경원PSD[(39.83 ±9.30)nm]변박(P＜0.05)、돌촉간극[(19.98±5.21)nm]증관(P＜0.05)、돌촉계면곡솔(1.12±0.05)하강(P＜0.05)；여모형조비교,침자조소서돌촉PSD[(46.78±11.37)nm]증후(P＜0.05)、돌촉간극[(15.68±4.03)nm]축소(P＜0.05)、돌촉계면곡솔(1.14 ±0.09)증대(P＜0.05).모형조소서해마GluR1함량교대조조하강(P＞0.05),침자조교모형조GluR1함량증가(P＞0.05),모형조GluR2함량교대조조하강(P＜0.05),침자조교모형조GluR2함량증가(P＜0.05).결론 침자가현저제고SAMP8적학습기억능력급돌촉가소성,병촉진기해마AMPA수체GluR2아기표체,제시침자가능시촉진신경강복、개선학습기억능력적방법지일.
Objective To observe any effect of acupuncture on AMPA receptor expression and synaptic plasticity in the hippocampal neurons of mice prone to accelerated senescence (SAMP8s).Methods Nineteen SAMP8s aged 9 months were randomly divided into a model group (n =10) and an acupuncture group (n =9).Nine mice resistant to accelerated senescence (SAMR1s) were used as a control group.Acupuncture was administered daily on the DU20 (Baihui) and K1 (Yongquan) acupoints,7 days in each course for 4 courses,with a 2 day interval between courses.The Morris water maze was used to observe learning and memory abilities.The mice were sacrificed after the last treatment.Transmission electron microscopy was used to observe the synaptic ultrastructure in subregion CA1 of the hippocampus.Western blotting was used to detect the expression of the AMPA receptor subunits GluR1 and GluR2.Results Compared to the control group,escaping latency was prolonged significantly in the model group but decreased in the acupuncture group.Target annulus crossings decreased significantly in the model group but increased significantly in the acupuncture group.Compared to the model group,the average thickness of the post-synaptic density (PSD) and the curvature of the synaptic interface both increased significantly in the acupuncture group,while the average synapse width decreased significantly.There were no significant differences in GluR1 expression among the groups,but compared to the control group,GluR2 expression decreased significantly in the model group but was significantly up-regulated in the acupuncture group.Conclusions Acupuncture can improve learning and memory,facilitate synaptic plasticity and up-regulate GluR2 expression in hippocampal neurons,at least among SAMP8 rats.