中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
3期
161-164
,共4页
魏居瑞%刘喆%刘佩%夏玮%何方%吴杰%陈萍%骆碧君%谈胜国
魏居瑞%劉喆%劉珮%夏瑋%何方%吳傑%陳萍%駱碧君%談勝國
위거서%류철%류패%하위%하방%오걸%진평%락벽군%담성국
电针%海马%学习记忆
電針%海馬%學習記憶
전침%해마%학습기억
Electro-acupuncture%Hippocampus%Learning%Memory
目的 观察电针(EA)对慢性脑缺血大鼠学习记忆功能和海马组织头蛋白(Noggin) mRNA表达的影响.方法 选取雄性Sprague-Dawley(SD)大鼠120只,采用改良永久性结扎双侧颈总动脉法制作慢性脑缺血模型.将造模成功的104只大鼠按随机数字表法分为模型组和电针组,每组大鼠52只,每组再根据取材的时间点分为造模成功后第1、2、4、6周4个亚组,每个时间点取13只大鼠.电针组采用电针治疗,模型组仅常规饲养.2组大鼠均于取材前5d行Morris水迷宫检测,并在对应的时间点(造模成功后第1、2、4、6周)按随机数字表法各亚组抽取6只大鼠.采用逆转录-聚合酶链式反应(RT-PCR)方法半定量检测大鼠海马中Noggin mRNA的表达情况.结果 造模成功后第2、4、6周,电针组的逃避潜伏期分别为(23.8 ±4.16)s、(23.7 ±7.28)s、(22.26 ±4.90)s,与模型组同时间点的(32.21±7.91)s、(32.91±11.68)s、(30.11 ±5.76)s比较,差异均有统计学意义(P<0.05).造模成功后第2、4、6周,电针组的各时间点第一象限内游泳时间与模型组同时间点比较,差异均有统计学意义(P<0.05).电针组造模后各时间点的Noggin mRNA水平均高于模型组同时间点(P<0.05);造模后第6周,电针组Noggin mRNA的表达显著低于组内各时间点(P<0.05).结论 电针可改善慢性脑缺血大鼠空间学习能力和记忆能力,提高慢性脑缺血大鼠海马Noggin mRNA的表达.
目的 觀察電針(EA)對慢性腦缺血大鼠學習記憶功能和海馬組織頭蛋白(Noggin) mRNA錶達的影響.方法 選取雄性Sprague-Dawley(SD)大鼠120隻,採用改良永久性結扎雙側頸總動脈法製作慢性腦缺血模型.將造模成功的104隻大鼠按隨機數字錶法分為模型組和電針組,每組大鼠52隻,每組再根據取材的時間點分為造模成功後第1、2、4、6週4箇亞組,每箇時間點取13隻大鼠.電針組採用電針治療,模型組僅常規飼養.2組大鼠均于取材前5d行Morris水迷宮檢測,併在對應的時間點(造模成功後第1、2、4、6週)按隨機數字錶法各亞組抽取6隻大鼠.採用逆轉錄-聚閤酶鏈式反應(RT-PCR)方法半定量檢測大鼠海馬中Noggin mRNA的錶達情況.結果 造模成功後第2、4、6週,電針組的逃避潛伏期分彆為(23.8 ±4.16)s、(23.7 ±7.28)s、(22.26 ±4.90)s,與模型組同時間點的(32.21±7.91)s、(32.91±11.68)s、(30.11 ±5.76)s比較,差異均有統計學意義(P<0.05).造模成功後第2、4、6週,電針組的各時間點第一象限內遊泳時間與模型組同時間點比較,差異均有統計學意義(P<0.05).電針組造模後各時間點的Noggin mRNA水平均高于模型組同時間點(P<0.05);造模後第6週,電針組Noggin mRNA的錶達顯著低于組內各時間點(P<0.05).結論 電針可改善慢性腦缺血大鼠空間學習能力和記憶能力,提高慢性腦缺血大鼠海馬Noggin mRNA的錶達.
목적 관찰전침(EA)대만성뇌결혈대서학습기억공능화해마조직두단백(Noggin) mRNA표체적영향.방법 선취웅성Sprague-Dawley(SD)대서120지,채용개량영구성결찰쌍측경총동맥법제작만성뇌결혈모형.장조모성공적104지대서안수궤수자표법분위모형조화전침조,매조대서52지,매조재근거취재적시간점분위조모성공후제1、2、4、6주4개아조,매개시간점취13지대서.전침조채용전침치료,모형조부상규사양.2조대서균우취재전5d행Morris수미궁검측,병재대응적시간점(조모성공후제1、2、4、6주)안수궤수자표법각아조추취6지대서.채용역전록-취합매련식반응(RT-PCR)방법반정량검측대서해마중Noggin mRNA적표체정황.결과 조모성공후제2、4、6주,전침조적도피잠복기분별위(23.8 ±4.16)s、(23.7 ±7.28)s、(22.26 ±4.90)s,여모형조동시간점적(32.21±7.91)s、(32.91±11.68)s、(30.11 ±5.76)s비교,차이균유통계학의의(P<0.05).조모성공후제2、4、6주,전침조적각시간점제일상한내유영시간여모형조동시간점비교,차이균유통계학의의(P<0.05).전침조조모후각시간점적Noggin mRNA수평균고우모형조동시간점(P<0.05);조모후제6주,전침조Noggin mRNA적표체현저저우조내각시간점(P<0.05).결론 전침가개선만성뇌결혈대서공간학습능력화기억능력,제고만성뇌결혈대서해마Noggin mRNA적표체.
Objective To observe the effects of electro-acupuncture (EA) on learning,memory and the expression of Noggin mRNA in the hippocampus after cerebral ischemia.Methods One hundred and four male Sprague-Dawley (SD) rats were subjected to a modified form of permanent bilateral carotid artery ligation to produce a model of chronic cerebral ischemia.They were then randomly divided into a model group and an EA group,with 52 rats in each group.Each group was then subdivided into 1,2,4 and 6 weeks subgroups,with 13 rats in each subgroup.The EA group received EA administered to the Baihui (DU20) and Dazhui (BU14) acu-points; the model group received regular feeding only.The Morris water maze test was administered in both groups at 5 days before the rats were sacrificed.At the 1,2,4 and 6 week time points,6 rats were selected from each subgroup randomly and the expression of Noggin mRNA in the hippocampus was detected using RT-PCR techniques.Results The Morris water maze test showed significantly lower escape latency in the EA group at 2,4 and 6 weeks.Swimming time in the first quadrant was also significantly different at all time points.The average level of Noggin mRNA in the EA group was significantly higher than that in the model group at all of the time points.After 6 weeks,the average level of Noggin mRNA in the EA group was significantly lower than that at the other time points within the EA group.Conclusion EA can improve spatial learning and memory and elevate the expression of Noggin mRNA in the hippocampus in cases of chronic cerebral ischemia.