CAJ | 학술논문

目的探讨褪黑素(MT)对大鼠急性脊髓损伤后白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和白细胞介素-10(IL-10)表达的影响及其对损伤后炎症反应和神经修复的作用.方法选取成年SD大鼠108只,按随机数字表法分为脊髓损伤组、MT治疗组和假手术组,每组36只.脊髓损伤组和MT治疗组采用改良垂直打击(WD)法(打击量5X10 g.cm)建立T12脊髓损伤模型,假手术组只切除T12椎板不损伤脊髓.脊髓损伤组和MT治疗组在脊髓损伤后10 min分别注射含5％乙醇的生理盐水和MT制剂(按大鼠体重100 mg/kg计算).分别于模型建立手术后6、12、18、24和48 h从每组大鼠中按随机数字表选取6只大鼠,取脊髓标本及血液,并检测血清中IL-6、IL-8和IL-10含量及脊髓中IL-10 mRNA的表达水平.术后18h时,再分别于3组中选取6只行主动脉灌流后取出脊髓标本甲醛固定,切片后行HE染色观察大体变化,用免疫组化SP法观察IL-10的表达特点.结果术后6、12、18、24和48 h脊髓内IL-10 mRNA水平和血清中IL-10含量对比,褪黑素治疗组均最高、脊髓损伤组次之、假手术组最低,各组间其差异均有统计学意义(P＜0.01);相应时间点脊髓损伤组的IL-6和IL-8水平最高、MT治疗组次之、假手术组最低,各组间差异均有统计学意义(P＜0.01).术后18h脊髓标本中IL-10 mRNA阳性细胞数(个/高倍视野)对比,MT治疗组最高(5.7±0.80)、脊髓损伤组次之(4.6±0.83),假手术组最低(3.8±0.77),各组间差异均有统计学意义(P＜0.01).组织形态学观察显示MT治疗后脊髓中央管及其周围的灰质IL-10水平改善.结论 MT可以通过提高IL-10的表达及抑制IL-6和IL-8的表达来改善损伤后神经的脂质过氧化反应和炎症反应来修复损伤神经.
목적 탐토퇴흑소(MT)대대서급성척수손상후백세포개소-6(IL-6)、백세포개소-8(IL-8)화백세포개소-10(IL-10)표체적영향급기대손상후염증반응화신경수복적작용.방법 선취성년SD대서108지,안수궤수자표법분위척수손상조、MT치료조화가수술조,매조36지.척수손상조화MT치료조채용개량수직타격(WD)법(타격량5X10 g.cm)건립T12척수손상모형,가수술조지절제T12추판불손상척수.척수손상조화MT치료조재척수손상후10 min분별주사함5％을순적생리염수화MT제제(안대서체중100 mg/kg계산).분별우모형건립수술후6、12、18、24화48 h종매조대서중안수궤수자표선취6지대서,취척수표본급혈액,병검측혈청중IL-6、IL-8화IL-10함량급척수중IL-10 mRNA적표체수평.술후18h시,재분별우3조중선취6지행주동맥관류후취출척수표본갑철고정,절편후행HE염색관찰대체변화,용면역조화SP법관찰IL-10적표체특점.결과 술후6、12、18、24화48 h척수내IL-10 mRNA수평화혈청중IL-10함량대비,퇴흑소치료조균최고、척수손상조차지、가수술조최저,각조간기차이균유통계학의의(P＜0.01);상응시간점척수손상조적IL-6화IL-8수평최고、MT치료조차지、가수술조최저,각조간차이균유통계학의의(P＜0.01).술후18h척수표본중IL-10 mRNA양성세포수(개/고배시야)대비,MT치료조최고(5.7±0.80)、척수손상조차지(4.6±0.83),가수술조최저(3.8±0.77),각조간차이균유통계학의의(P＜0.01).조직형태학관찰현시MT치료후척수중앙관급기주위적회질IL-10수평개선.결론 MT가이통과제고IL-10적표체급억제IL-6화IL-8적표체래개선손상후신경적지질과양화반응화염증반응래수복손상신경.
Objective To explore the effects of melatonin (MT) on the expression of interleukin (IL)-10,interleukin-6 and interleukin-8 as well as the inflammatory reaction and nerve repair after acute spinal cord injury (SCI).Methods One hundred and eight Sprague-Dawley rats were randomly divided into a spinal cord injury group (group A),an MT treatment group (group B) and a sham operation group (group C),each with 36 rats.SCI models were established in the rats of groups A and B using a version of Allen's weight drop method (50gcf at the T12 level).Group C had removal of the lamina only.Ten minutes later,group A was injected with 5％ ethanol in saline (the MT solvent) and group B with 100 mg/kg of melatonin preparation.At 6,12,18 and 24 hours,IL-6,IL-8 and IL-10 levels in serum were detected in 6 rats of each group.At 18 hours post-surgery,spinal cord specimens were taken from 6 rats of each group for hematoxylin eosin staining,morphological examination and immunohistochemical SP detection of IL-10 expression.Results The specimens of group A showed inflammatory reaction and ulceration at 48 h; groups B and C had no ulcers.Group B showed the highest levels of IL-10 in serum and IL-10 mRNA in the spinal cord,while group C showed the lowest level.The differences were statistically significant.Group A had the highest levels of IL-6 and IL-8 and group C had the lowest.The difference between group B and groups A and C was significant.The morpho-logical observation showed that after melatonin treatment the IL-10 levels in the spinal cord's central canal and around the gray matter improved.Conclusions Melatonin can improve nerve lipid peroxidation and inflammatory reaction in the treatment of spinal cord injury by increasing IL-10 expression and inhibiting IL-6 and IL-8 expression.