中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
6期
401-406
,共6页
王晓林%王庸晋%荣书玲%马红彪
王曉林%王庸晉%榮書玲%馬紅彪
왕효림%왕용진%영서령%마홍표
运动%高血压%心室重构%大鼠
運動%高血壓%心室重構%大鼠
운동%고혈압%심실중구%대서
Exercise%Hypertension%Ventricular remodeling%Rat
目的 观察运动训练对自发性高血压大鼠(SHR)左心室重构的影响.方法 将20只8周龄雄性SHR按随机数字表法分为高血压对照组和高血压运动组,每组10只;另选取8周龄健康雄性Wistar大鼠10只作为正常对照组.高血压运动组大鼠进行为期12周低强度的无负重游泳运动,每周6次、每次60 min;高血压对照组和正常对照组大鼠仅常规饲养,不进行任何训练.3组大鼠每周测量1次血压,运动12周后ELISA检测血清中去甲肾上腺素(NE)和血管紧张素Ⅱ(ANGⅡ)水平.组织学检测左心室心肌形态学的变化及心肌细胞直径,天狼星红染色检测左心室胶原沉积,半定量逆转录-聚合酶链反应(RT-PCR)和免疫印迹检测重塑的左心室心肌组织中肿瘤坏死因子-α(TNF-α),白介素-6(IL-6),白介素-1β(IL-1β)和转化生长因子β1(TGF-β1)的表达.结果 入组12周后,高血压运动组的收缩压和舒张压分别为(150.6±6.7) mmHg和(91.6±7.1) mmHg,与组内入组后当天和高血压对照组入组12周后比较,均显著下降(P＜0.05),但与正常对照组比较,差异仍有统计学意义(P＜0.05).入组12周后,高血压运动组的LVW/BW、NE和ANGⅡ水平显著低于高血压对照组大鼠(P＜0.05),但仍显著高于正常对照组大鼠(P＜0.05);高血压运动组和高血压对照组大鼠的左心室心肌细胞直径均显著大于正常对照组(P＜0.05),而高血压对照组的左心室心肌细胞直径亦大于高血压运动组(P＜0.05);高血压运动组和高血压对照组大鼠的胶原容积分数(CvF)值均显著高于正常对照组(P＜0.05),而高血压对照组的CVF值亦显著高于高血压运动组(P＜0.05).入组12周后,高血压运动组和高血压对照组重塑的左心室心肌中TNF-α,IL-6,IL-1β和TGF-β1的mRNA及蛋白的表达均显著高于正常对照组(P＜0.05);高血压运动组重塑的左心室心肌中TNF-α,IL-6,IL-1β和TGF-β1的mRNA及蛋白的表达均显著低于高血压对照组(P＜0.05).结论 游泳运动可显著降低SHR的血压水平,改善SHR大鼠的左心室重构,其改善左心室重构的机制不仅与降低交感神经与肾素-血管紧张素系统的活性有关,还与降低细胞因子TNF-α,IL-6,IL-1β和TGF-β1的表达有关.
目的 觀察運動訓練對自髮性高血壓大鼠(SHR)左心室重構的影響.方法 將20隻8週齡雄性SHR按隨機數字錶法分為高血壓對照組和高血壓運動組,每組10隻;另選取8週齡健康雄性Wistar大鼠10隻作為正常對照組.高血壓運動組大鼠進行為期12週低彊度的無負重遊泳運動,每週6次、每次60 min;高血壓對照組和正常對照組大鼠僅常規飼養,不進行任何訓練.3組大鼠每週測量1次血壓,運動12週後ELISA檢測血清中去甲腎上腺素(NE)和血管緊張素Ⅱ(ANGⅡ)水平.組織學檢測左心室心肌形態學的變化及心肌細胞直徑,天狼星紅染色檢測左心室膠原沉積,半定量逆轉錄-聚閤酶鏈反應(RT-PCR)和免疫印跡檢測重塑的左心室心肌組織中腫瘤壞死因子-α(TNF-α),白介素-6(IL-6),白介素-1β(IL-1β)和轉化生長因子β1(TGF-β1)的錶達.結果 入組12週後,高血壓運動組的收縮壓和舒張壓分彆為(150.6±6.7) mmHg和(91.6±7.1) mmHg,與組內入組後噹天和高血壓對照組入組12週後比較,均顯著下降(P＜0.05),但與正常對照組比較,差異仍有統計學意義(P＜0.05).入組12週後,高血壓運動組的LVW/BW、NE和ANGⅡ水平顯著低于高血壓對照組大鼠(P＜0.05),但仍顯著高于正常對照組大鼠(P＜0.05);高血壓運動組和高血壓對照組大鼠的左心室心肌細胞直徑均顯著大于正常對照組(P＜0.05),而高血壓對照組的左心室心肌細胞直徑亦大于高血壓運動組(P＜0.05);高血壓運動組和高血壓對照組大鼠的膠原容積分數(CvF)值均顯著高于正常對照組(P＜0.05),而高血壓對照組的CVF值亦顯著高于高血壓運動組(P＜0.05).入組12週後,高血壓運動組和高血壓對照組重塑的左心室心肌中TNF-α,IL-6,IL-1β和TGF-β1的mRNA及蛋白的錶達均顯著高于正常對照組(P＜0.05);高血壓運動組重塑的左心室心肌中TNF-α,IL-6,IL-1β和TGF-β1的mRNA及蛋白的錶達均顯著低于高血壓對照組(P＜0.05).結論 遊泳運動可顯著降低SHR的血壓水平,改善SHR大鼠的左心室重構,其改善左心室重構的機製不僅與降低交感神經與腎素-血管緊張素繫統的活性有關,還與降低細胞因子TNF-α,IL-6,IL-1β和TGF-β1的錶達有關.
목적 관찰운동훈련대자발성고혈압대서(SHR)좌심실중구적영향.방법 장20지8주령웅성SHR안수궤수자표법분위고혈압대조조화고혈압운동조,매조10지;령선취8주령건강웅성Wistar대서10지작위정상대조조.고혈압운동조대서진행위기12주저강도적무부중유영운동,매주6차、매차60 min;고혈압대조조화정상대조조대서부상규사양,불진행임하훈련.3조대서매주측량1차혈압,운동12주후ELISA검측혈청중거갑신상선소(NE)화혈관긴장소Ⅱ(ANGⅡ)수평.조직학검측좌심실심기형태학적변화급심기세포직경,천랑성홍염색검측좌심실효원침적,반정량역전록-취합매련반응(RT-PCR)화면역인적검측중소적좌심실심기조직중종류배사인자-α(TNF-α),백개소-6(IL-6),백개소-1β(IL-1β)화전화생장인자β1(TGF-β1)적표체.결과 입조12주후,고혈압운동조적수축압화서장압분별위(150.6±6.7) mmHg화(91.6±7.1) mmHg,여조내입조후당천화고혈압대조조입조12주후비교,균현저하강(P＜0.05),단여정상대조조비교,차이잉유통계학의의(P＜0.05).입조12주후,고혈압운동조적LVW/BW、NE화ANGⅡ수평현저저우고혈압대조조대서(P＜0.05),단잉현저고우정상대조조대서(P＜0.05);고혈압운동조화고혈압대조조대서적좌심실심기세포직경균현저대우정상대조조(P＜0.05),이고혈압대조조적좌심실심기세포직경역대우고혈압운동조(P＜0.05);고혈압운동조화고혈압대조조대서적효원용적분수(CvF)치균현저고우정상대조조(P＜0.05),이고혈압대조조적CVF치역현저고우고혈압운동조(P＜0.05).입조12주후,고혈압운동조화고혈압대조조중소적좌심실심기중TNF-α,IL-6,IL-1β화TGF-β1적mRNA급단백적표체균현저고우정상대조조(P＜0.05);고혈압운동조중소적좌심실심기중TNF-α,IL-6,IL-1β화TGF-β1적mRNA급단백적표체균현저저우고혈압대조조(P＜0.05).결론 유영운동가현저강저SHR적혈압수평,개선SHR대서적좌심실중구,기개선좌심실중구적궤제불부여강저교감신경여신소-혈관긴장소계통적활성유관,환여강저세포인자TNF-α,IL-6,IL-1β화TGF-β1적표체유관.
Objective To investigate the effects of swimming exercise training on left ventricular (LV)remodeling and its possible mechanism in spontaneous hypertensive rats (SHR).Methods Twenty eightweek-old male SHRs were divided into SHR control (SC) group and SHR exercise training (ST) group,with 10 rats in each group.Ten eight-week-old male Wistar rats were used as normal control (WC) group.The ST group was subject to 60-min moderate swimming exercise without loading once daily,6 times a week,for a total of 12 weeks; while the SC and WC group had no special intervention.The blood pressure was examined once weekly.After 12 weeks,the norepinephrine (NE)and serum angiotonin Ⅱ (ANG Ⅱ) levels were determined by ELISA.The LV hypertrophy was assessed by analysing the ratio of LV weights to body weights (LVW/BW) and cardiomyocyte diameter.The collagen deposited in LV was detected by sirius red staining.The expressions of tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in LV were determined by semi-quantitative real time-polymerase chain reaction (RT-PCR) and Western blotting.Results After 12 weeks,the blood pressure,serum NE and ANG Ⅱ levels,LVW/BW ratio,cardiomyocyte diameter and collagen volume fraction (CVF) increased significantly in SC group compared with WC group; while those in ST group decreased significantly.In addition,in ST group the mRNA and protein expressions of TNF-α,IL-6,IL-1 βand TGF-β1 also decreased significantly.Conclusions The swimming exercise could reduce the blood pressure of SHR and improve LV remodeling.This effect was mediated not only by improving the hemodynamics,but also by decreasing sympathetic nerve and renin-angiotensin system (RAS) activities,decreasing the gene expressions of cytokines.The swimming exercise may be an effective strategy for improving LV remodeling in hypertension.
