CAJ | 학술논문

目的观察高压氧(HBO)联合中药冰片对大鼠血脑屏障(BBB)丙戊酸钠透过率的影响.方法选取56只健康SD大鼠,按照随机数字表法将其分为对照组(腹腔注射丙戊酸钠0.1 g/kg)、HBO-1组(HBO治疗结束后0.5h注射丙戊酸钠0.1 g/kg)、HBO-2组(HBO治疗同时注射丙戊酸钠0.1 g/kg)、HBO-3组(HBO治疗前0.5h注射丙戊酸钠0.1 g/kg)、低剂量冰片组(灌服0.125 g/kg冰片0.5h后注射丙戊酸钠0.1 g/kg)、高剂量冰片组(灌服0.25 g/kg冰片0.5h后注射丙戊酸钠0.1 g/kg)、联合应用组(灌服0.125 g/kg冰片0.5h后注射丙戊酸钠0.1 g/kg,0.5h后进行HBO治疗),每组8只.注射丙戊酸钠1.5h后,取大鼠的血液及脑脊液,采用高效液相色谱法测定丙戊酸钠的浓度.结果与对照组比较,HBO-2组[(97.43 ±12.09)mg/L]、HBO-3组[(100.10±13.54) mg/L]大鼠脑脊液丙戊酸钠浓度均显著增加,差异有统计学意义(P＜0.05),脑脊液血浆浓度比也明显增高(P＜0.05);与对照组比较,高剂量冰片组大鼠脑脊液丙戊酸钠浓度[(91.09±9.45)mg/L]及脑脊液血浆浓度比值(0.577±0.051)较高,差异有统计学意义(P＜0.05).与低剂量冰片组比较,HBO-3组、联合应用组大鼠脑脊液丙戊酸钠浓度较高,差异有统计学意义(P＜0.05).与HBO-3组比较,联合应用组大鼠脑脊液丙戊酸钠浓度[(112.43±11.52)mg/L]及脑脊液血浆浓度比(0.698±0.058)均显著增加(P＜0.05).各组间血液丙戊酸钠浓度比较,差异均无统计学意义(P＞0.05).结论 HBO疗法可提高大鼠BBB对丙戊酸钠的通透性,与低剂量冰片联用后效果更佳.
목적 관찰고압양(HBO)연합중약빙편대대서혈뇌병장(BBB)병무산납투과솔적영향.방법 선취56지건강SD대서,안조수궤수자표법장기분위대조조(복강주사병무산납0.1 g/kg)、HBO-1조(HBO치료결속후0.5h주사병무산납0.1 g/kg)、HBO-2조(HBO치료동시주사병무산납0.1 g/kg)、HBO-3조(HBO치료전0.5h주사병무산납0.1 g/kg)、저제량빙편조(관복0.125 g/kg빙편0.5h후주사병무산납0.1 g/kg)、고제량빙편조(관복0.25 g/kg빙편0.5h후주사병무산납0.1 g/kg)、연합응용조(관복0.125 g/kg빙편0.5h후주사병무산납0.1 g/kg,0.5h후진행HBO치료),매조8지.주사병무산납1.5h후,취대서적혈액급뇌척액,채용고효액상색보법측정병무산납적농도.결과 여대조조비교,HBO-2조[(97.43 ±12.09)mg/L]、HBO-3조[(100.10±13.54) mg/L]대서뇌척액병무산납농도균현저증가,차이유통계학의의(P＜0.05),뇌척액혈장농도비야명현증고(P＜0.05);여대조조비교,고제량빙편조대서뇌척액병무산납농도[(91.09±9.45)mg/L]급뇌척액혈장농도비치(0.577±0.051)교고,차이유통계학의의(P＜0.05).여저제량빙편조비교,HBO-3조、연합응용조대서뇌척액병무산납농도교고,차이유통계학의의(P＜0.05).여HBO-3조비교,연합응용조대서뇌척액병무산납농도[(112.43±11.52)mg/L]급뇌척액혈장농도비(0.698±0.058)균현저증가(P＜0.05).각조간혈액병무산납농도비교,차이균무통계학의의(P＞0.05).결론 HBO요법가제고대서BBB대병무산납적통투성,여저제량빙편련용후효과경가.
Objective To observe the effect of hyperbaric oxygen (HBO) combined with borneol on the permeability of the blood-brain barrier to sodium valproate in rats.Methods Fifty-six male,healthy Sprague Dawley rats were randomly divided into a control group which received an intraperitoneal injection of sodium valproate (0.1 g/kg),an HBO-1 group to which HBO was administered 0.5 hours before an equivalent injection of sodium valproate,an HBO-2 group where the HBO and sodium valproate were administered together,an HBO-3 group which received HBO 0.5 hours after the injection,a low dosage borneol group (L-borneol) which received 0.125 g/kg of borneol by oral perfusion 0.5 hours before an injection of sodium valproate (0.1 g/kg),a high dosage borneol group which received 0.25 g/kg of borneol 0.5 hours before an injection of sodium valproate (0.1 g/kg) and a combined group which received the HBO-3 group's treatment supplemented with the borneol dose of the L-borneol group.There were 8 rats in each group.Blood and cerebrospinal fluid (CSF) were collected 1.5 h after the sodium valproate injection and the concentrations of sodium valproate were measured by high performance liquid chromatography.Results Compared with the control group,the CSF concentrations of sodium valproate in the HBO-2 and HBO-3 groups had increased significantly more (to 97.43 ± 12.09 mg/L and 100.10 ± 13.54 mg/L respectively).The ratios of the CSF to plasma (C/P) concentrations had also increased significantly more.The CSF concentration of sodium valproate in the H-borneol group (91.09 ± 9.45 mg/L) and the C/P ratio (0.577 ± 0.051) had increased significantly more than in the control group.Compared with the L-borneol group,there were significantly greater increases in the HBO-3 group and the combined group in the concentration of sodium valproate in the rats' CSF.Compared with the HBO-3 group,the average concentration of sodium valproate in the CSF of the combined group (112.43 ± 11.52 mg/L) and the C/P ratio (0.698 ±0.058) had increased significantly more.There was no significant difference among the groups in the plasma concentrations of sodium valproate.Conclusions HBO can increase the permeability of the blood-brain barrier to sodium valproate,at least in rats.Combining HBO with a low dose of borneol has an additional effect.