中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
9期
671-675
,共5页
汪琦%王伟%潘邓记%朱舟%陈黛琪
汪琦%王偉%潘鄧記%硃舟%陳黛琪
왕기%왕위%반산기%주주%진대기
阿尔茨海默病%β淀粉样蛋白%小胶质细胞%基质细胞衍生因子-1%趋化作用
阿爾茨海默病%β澱粉樣蛋白%小膠質細胞%基質細胞衍生因子-1%趨化作用
아이자해묵병%β정분양단백%소효질세포%기질세포연생인자-1%추화작용
Alzheimer's disease%Beta amyloid%Microglia%Stromal cell derived factor-1%Chemo-taxis
目的 观察基质细胞衍生因子1(SDF-1)对阿尔茨海默病小鼠脑内β淀粉样蛋白(Aβ)的清除作用,并研究其作用机制.方法 将12只APP/PS转基因小鼠按随机数字表法分为治疗组和对照组,治疗组给予侧脑室SDF-1注射,对照组给予侧脑室磷酸缓冲盐溶液(PBS)注射,每周1次,连续注射8周.治疗8周后,采用免疫荧光组织化学方法观察并比较2组注射后小鼠脑内Aβ斑块的数量和面积,以及小胶质细胞分布及斑块相关的小胶质细胞的数量.结果 治疗8周后,治疗组小鼠海马区域Aβ斑块的相对面积和数量为(0.4527±0.0711)％和(0.004 837±0.001 074)％,与对照组比较,差异均有统计学意义(P＜0.05);治疗组小鼠皮质区域Aβ斑块的相对面积和数量与对照组比较,差异亦均有统计学意义(P＜0.05).治疗8周后,治疗组海马和皮质区域斑块相关的小胶质细胞的数量与对照组比较,差异均有统计学意义(P＜0.05).结论 SDF-1α侧脑室注射可能减少APP/PS1小鼠脑内Aβ的斑块,其作用机制可能是SDF-1增加了小胶质细胞向Aβ斑块的趋化作用,从而促进Aβ斑块的吞噬清除.
目的 觀察基質細胞衍生因子1(SDF-1)對阿爾茨海默病小鼠腦內β澱粉樣蛋白(Aβ)的清除作用,併研究其作用機製.方法 將12隻APP/PS轉基因小鼠按隨機數字錶法分為治療組和對照組,治療組給予側腦室SDF-1註射,對照組給予側腦室燐痠緩遲鹽溶液(PBS)註射,每週1次,連續註射8週.治療8週後,採用免疫熒光組織化學方法觀察併比較2組註射後小鼠腦內Aβ斑塊的數量和麵積,以及小膠質細胞分佈及斑塊相關的小膠質細胞的數量.結果 治療8週後,治療組小鼠海馬區域Aβ斑塊的相對麵積和數量為(0.4527±0.0711)％和(0.004 837±0.001 074)％,與對照組比較,差異均有統計學意義(P＜0.05);治療組小鼠皮質區域Aβ斑塊的相對麵積和數量與對照組比較,差異亦均有統計學意義(P＜0.05).治療8週後,治療組海馬和皮質區域斑塊相關的小膠質細胞的數量與對照組比較,差異均有統計學意義(P＜0.05).結論 SDF-1α側腦室註射可能減少APP/PS1小鼠腦內Aβ的斑塊,其作用機製可能是SDF-1增加瞭小膠質細胞嚮Aβ斑塊的趨化作用,從而促進Aβ斑塊的吞噬清除.
목적 관찰기질세포연생인자1(SDF-1)대아이자해묵병소서뇌내β정분양단백(Aβ)적청제작용,병연구기작용궤제.방법 장12지APP/PS전기인소서안수궤수자표법분위치료조화대조조,치료조급여측뇌실SDF-1주사,대조조급여측뇌실린산완충염용액(PBS)주사,매주1차,련속주사8주.치료8주후,채용면역형광조직화학방법관찰병비교2조주사후소서뇌내Aβ반괴적수량화면적,이급소효질세포분포급반괴상관적소효질세포적수량.결과 치료8주후,치료조소서해마구역Aβ반괴적상대면적화수량위(0.4527±0.0711)％화(0.004 837±0.001 074)％,여대조조비교,차이균유통계학의의(P＜0.05);치료조소서피질구역Aβ반괴적상대면적화수량여대조조비교,차이역균유통계학의의(P＜0.05).치료8주후,치료조해마화피질구역반괴상관적소효질세포적수량여대조조비교,차이균유통계학의의(P＜0.05).결론 SDF-1α측뇌실주사가능감소APP/PS1소서뇌내Aβ적반괴,기작용궤제가능시SDF-1증가료소효질세포향Aβ반괴적추화작용,종이촉진Aβ반괴적탄서청제.
Objective To explore whether stromal cell-derived factor 1 (SDF-1) can promote the clearance of β-amyloid deposition in the brain of APP/PS1 mice and the possible underlying mechanism.Methods Twelve 28-week-old APP/PS1 mice were divided into two groups:a treatment group and a control group.Animals were given the intracerebroventricular injection weekly with PBS or mouse recombinant SDF-1 α for eight weeks.Microglia and Aβ in cerebral cortex and hippocampal region of APP/PS1 mice were detected by immunofluorescence.Results After 8-week treatment,both the relative number and the relative area of Aβ deposits in the mice of treatment group were less than those in the control group.The relative number of plaque associated microglia increased to a significantly greater extent in the cortex and hippocampus in treatment group than those in the control group.Conclusion Injecting SDF-1α significantly reduced amyloid burden in APP/PS1 mice.This effect might associated with the improvement of the chemotoxis of microglia,which promote the phagocytosis of Aβ by microglia.
