CAJ | 학술논문

目的研究北京市2006年新确认HIV-1感染者毒株的耐药突变本底数据.方法随机选取北京市2006年新确认HIV-1感染者抗凝全血标本50份,提取血浆病毒RNA,用逆转录聚合酶链反应扩增HIV-1 pol区基因片段,并进行序列测定及耐药基因型分析.结果成功扩增出34份标本的pol区基因;在1例样本的蛋白酶编码区检测出1个主要耐药突变,7例样本检测出7个次要耐药突变,主要耐药突变为M46L,毒株是CRF01_AE亚型,次要耐药突变有4种,出现的频率分别为A71T(2个)、A71V(3个)、Q58E(1个)、V11IV(1个).在14例样本逆转录酶编码区检测出一种或多种核苷类和(或)非核苷类逆转录酶抑制剂耐药突变,9例标本检出核苷类逆转录酶抑制剂耐药突变,出现频率分别为:V118I(42.9%)、M184V(7.1%)、A62V(7.1%)、K70T(7.1%)、K65R(7.1%)、K219N(7.1%)、T69d(7.1%)、V75LV(7.1%)、K219R(7.1%);10例标本检出核苷类逆转录酶抑制剂耐药突变,出现的频率分别为V1061(35.5%)、Y181C(15.4%)、K103KR(7.7%)、K103R(7.7%)、L100LV(7.7%)、V1081(7.7%)、V179D(7.7%)、V179DV(7.7%).结论北京市2006年新确认HIV-1感染者毒株中已经存在一定比例耐药突变,有必要定期进行耐药性监测研究.
목적 연구북경시2006년신학인HIV-1감염자독주적내약돌변본저수거.방법 수궤선취북경시2006년신학인HIV-1감염자항응전혈표본50빈,제취혈장병독RNA,용역전록취합매련반응확증HIV-1 pol구기인편단,병진행서렬측정급내약기인형분석.결과 성공확증출34빈표본적pol구기인;재1례양본적단백매편마구검측출1개주요내약돌변,7례양본검측출7개차요내약돌변,주요내약돌변위M46L,독주시CRF01_AE아형,차요내약돌변유4충,출현적빈솔분별위A71T(2개)、A71V(3개)、Q58E(1개)、V11IV(1개).재14례양본역전록매편마구검측출일충혹다충핵감류화(혹)비핵감류역전록매억제제내약돌변,9례표본검출핵감류역전록매억제제내약돌변,출현빈솔분별위:V118I(42.9%)、M184V(7.1%)、A62V(7.1%)、K70T(7.1%)、K65R(7.1%)、K219N(7.1%)、T69d(7.1%)、V75LV(7.1%)、K219R(7.1%);10례표본검출핵감류역전록매억제제내약돌변,출현적빈솔분별위V1061(35.5%)、Y181C(15.4%)、K103KR(7.7%)、K103R(7.7%)、L100LV(7.7%)、V1081(7.7%)、V179D(7.7%)、V179DV(7.7%).결론 북경시2006년신학인HIV-1감염자독주중이경존재일정비례내약돌변,유필요정기진행내약성감측연구.
Objective To study the drug resistance mutations of reverse transcriptase and protease among HIV-1 strains of new confirmed HIV-1-infected patients in Beijing,provide the base-line data for clinical antiviral treatment in Beijing.Methods We amplified partial pol fiequences by RT-PCR and nestPCR.then sequenced the gene segment and phylogenetically analyzed.Drug-resistance associated mutations in protease and reverse transcriptase regions were analyzed with Stanford University HIV Drug Resistance Database.Results Totat of 34 pol gene sequences were obtained from 50 HIV-1-infected patients.Analysis of drug-resistance associated mutation showed that 15 of 34 persons infected with drug-resistant HIV-1,among which major mutations in PR and RT regions were 1 and 14 of 34 persons respectively.The frequencies of major mutation in PR region were M46L(100%)and the NRTI(nucleoside reverse ranscriptase inhibitor) and NNRTI(non-nucleoside reverse ranscriptase inhibitor)resistance mutations in RT region were V118I (42.9%),M184V(7.1%),A62V(7.1%),K70T(7.1%),K65R(7.1%),K219N(7.1%),T69d (7.1%),V75LV(7.1%),K219R(7.1%)and V106I(35.5%),Y181C(15.4%),K103KR(7.7%),K103R(7.7%),L100LV(7.7%),V1081(7.7%),V179D(7.7%),V179DV(7.7%),respectively.Conclusion There were drug resistance mutations in some new confirmed HIV-1-infected patient in this study.the surveillance of HIV-1 drug resistance mutations should be paid more attention.