中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
10期
903-906
,共4页
瞿秋霞%陈成%蒋玉平%陈曦%沈宇%朱一蓓%张学光
瞿鞦霞%陳成%蔣玉平%陳晞%瀋宇%硃一蓓%張學光
구추하%진성%장옥평%진희%침우%주일배%장학광
树突状细胞%B7-H4%T淋巴细胞
樹突狀細胞%B7-H4%T淋巴細胞
수돌상세포%B7-H4%T림파세포
Dendritic cell%B7-H4%T lymphocytes
目的 探讨B7-H4分子在小鼠树突状细胞分化发育过程中的表达及其在T细胞活化中的作用.方法 采用GM-CSF和IL-4联合方案体外诱导小鼠髓系树突状细胞(DCs);采用流式细胞术检测未成熟DCs、成熟DCs以及IL-10诱导的DCs表面B7-H4分子的表达;采用3H-TdR掺入试验和抗B7-H4单抗(McAb)阻断试验分析DCs表达的B7-H4分子对T淋巴细胞的共刺激效应.结果 经GM-CSF和IL-4联合方案体外诱导的未成熟DCs较高水平表达B7-H4,在IL-10作用下B7-H4表达进一步上调,经TNF-α刺激成熟后,B7-H4的表达显著下调,不同功能状态下DCs表面B7-H4分子均可抑制T细胞的增殖,但以未成熟DCs、IL-10诱导的DCs表面B7-H4分子的抑制作用更为显著.结论 不同功能状态下的DCs均有B7-H4分子的表达,处于抑制状态下的DCs通过高表达B7-H4介导免疫不应答效应.
目的 探討B7-H4分子在小鼠樹突狀細胞分化髮育過程中的錶達及其在T細胞活化中的作用.方法 採用GM-CSF和IL-4聯閤方案體外誘導小鼠髓繫樹突狀細胞(DCs);採用流式細胞術檢測未成熟DCs、成熟DCs以及IL-10誘導的DCs錶麵B7-H4分子的錶達;採用3H-TdR摻入試驗和抗B7-H4單抗(McAb)阻斷試驗分析DCs錶達的B7-H4分子對T淋巴細胞的共刺激效應.結果 經GM-CSF和IL-4聯閤方案體外誘導的未成熟DCs較高水平錶達B7-H4,在IL-10作用下B7-H4錶達進一步上調,經TNF-α刺激成熟後,B7-H4的錶達顯著下調,不同功能狀態下DCs錶麵B7-H4分子均可抑製T細胞的增殖,但以未成熟DCs、IL-10誘導的DCs錶麵B7-H4分子的抑製作用更為顯著.結論 不同功能狀態下的DCs均有B7-H4分子的錶達,處于抑製狀態下的DCs通過高錶達B7-H4介導免疫不應答效應.
목적 탐토B7-H4분자재소서수돌상세포분화발육과정중적표체급기재T세포활화중적작용.방법 채용GM-CSF화IL-4연합방안체외유도소서수계수돌상세포(DCs);채용류식세포술검측미성숙DCs、성숙DCs이급IL-10유도적DCs표면B7-H4분자적표체;채용3H-TdR참입시험화항B7-H4단항(McAb)조단시험분석DCs표체적B7-H4분자대T림파세포적공자격효응.결과 경GM-CSF화IL-4연합방안체외유도적미성숙DCs교고수평표체B7-H4,재IL-10작용하B7-H4표체진일보상조,경TNF-α자격성숙후,B7-H4적표체현저하조,불동공능상태하DCs표면B7-H4분자균가억제T세포적증식,단이미성숙DCs、IL-10유도적DCs표면B7-H4분자적억제작용경위현저.결론 불동공능상태하적DCs균유B7-H4분자적표체,처우억제상태하적DCs통과고표체B7-H4개도면역불응답효응.
Objective To study the role of B7-H4 on the surface of murine DCs in mediating T cell activation.Methods Mouse myetoid dendritic cells(DCs)were generated from bone marrow in vitro using GM-CSF and IL-4,and further stimulation with TNF-α or IL-10 for 48 h,respectively.Expressions of B7-H4 on DCs were analyzed by FCM.3H-thymidine incorporation test was used to detect the T cell proliferation stimulated by DCs with or without blocking B7-H4 by McAb.respectively.Results 5-6 d DCs in our system can be regarded as imDCs.B7-H4 was expressed moderately on imDCs,and could be markedly upregulated by IL-10,and be markedly down-regulated by TNF-α.3H-thymidine incorporation test showed that blockade of B7-H4 on imDCs and IL-10-treated DCs resulted in enhancement of T cell proliferation signiticantly.When T cells encountered with mDCs.B7-H4 blockade could not markedly enhance the T cells proliferation as seen in inhibitory DCs.Conclusion Expression of B7-H4 on inhibitory DCs might contribute to its immunoinhibitory effect.
