中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
10期
934-938
,共5页
张永宏%赵艳%徐克沂%闫惠平%马丽娜%伦文辉%吴昊%董涛%陈新月
張永宏%趙豔%徐剋沂%閆惠平%馬麗娜%倫文輝%吳昊%董濤%陳新月
장영굉%조염%서극기%염혜평%마려나%륜문휘%오호%동도%진신월
人免疫缺陷病毒-1%细胞免疫%重叠肽%酶联免疫斑点分析
人免疫缺陷病毒-1%細胞免疫%重疊肽%酶聯免疫斑點分析
인면역결함병독-1%세포면역%중첩태%매련면역반점분석
Human immunodeficiency virus-1 (HIV-1)%Cellular immunology%Overlapping peptides%Enzyme-linked immunospot assay (ELISPOT)
目的 探讨HIV-1特异性T细胞反应特征及对HIV-1感染者病情进展的影响.方法 通过合成重叠肽技术及ELISPOT技术,采用队列研究方法对37名HIV-1感染者的病毒特异性T细胞免疫反应进行分析.结果 83.78%(31/37)的HIV-1感染者对1个或多个合成肽反应(反应强度大于50 SFU/106 PBMCs).HIV-1B型病毒不同蛋白均可激发HIV-1感染者的特异性T细胞反应,其中HIV-1 Gag蛋白被识别的频率最高,81%的HIV-1感染者识别HIV-1 Gag而且HIV-1 Gag蛋白诱导的T细胞反应强度最高,相对强度达到28.25%,明显高于其他蛋白,差异具有统计学意义(F=17.969,P<0.001);重叠肽诱导T细胞分泌IFN-γ反应频率、反应强度在HIV-1感染无症状期和艾滋病期无明显区别,但是HIV-1 Gag蛋白诱导的T细胞反应强度在无症状期明显高于艾滋病期.结论 HIV-1B型病毒不同基因编码蛋白激发T细胞分泌IFN-γ反应小同,其中HIV-1 Gag蛋白特异性T细胞在控制病情进展方面发挥了重要作用.
目的 探討HIV-1特異性T細胞反應特徵及對HIV-1感染者病情進展的影響.方法 通過閤成重疊肽技術及ELISPOT技術,採用隊列研究方法對37名HIV-1感染者的病毒特異性T細胞免疫反應進行分析.結果 83.78%(31/37)的HIV-1感染者對1箇或多箇閤成肽反應(反應彊度大于50 SFU/106 PBMCs).HIV-1B型病毒不同蛋白均可激髮HIV-1感染者的特異性T細胞反應,其中HIV-1 Gag蛋白被識彆的頻率最高,81%的HIV-1感染者識彆HIV-1 Gag而且HIV-1 Gag蛋白誘導的T細胞反應彊度最高,相對彊度達到28.25%,明顯高于其他蛋白,差異具有統計學意義(F=17.969,P<0.001);重疊肽誘導T細胞分泌IFN-γ反應頻率、反應彊度在HIV-1感染無癥狀期和艾滋病期無明顯區彆,但是HIV-1 Gag蛋白誘導的T細胞反應彊度在無癥狀期明顯高于艾滋病期.結論 HIV-1B型病毒不同基因編碼蛋白激髮T細胞分泌IFN-γ反應小同,其中HIV-1 Gag蛋白特異性T細胞在控製病情進展方麵髮揮瞭重要作用.
목적 탐토HIV-1특이성T세포반응특정급대HIV-1감염자병정진전적영향.방법 통과합성중첩태기술급ELISPOT기술,채용대렬연구방법대37명HIV-1감염자적병독특이성T세포면역반응진행분석.결과 83.78%(31/37)적HIV-1감염자대1개혹다개합성태반응(반응강도대우50 SFU/106 PBMCs).HIV-1B형병독불동단백균가격발HIV-1감염자적특이성T세포반응,기중HIV-1 Gag단백피식별적빈솔최고,81%적HIV-1감염자식별HIV-1 Gag이차HIV-1 Gag단백유도적T세포반응강도최고,상대강도체도28.25%,명현고우기타단백,차이구유통계학의의(F=17.969,P<0.001);중첩태유도T세포분비IFN-γ반응빈솔、반응강도재HIV-1감염무증상기화애자병기무명현구별,단시HIV-1 Gag단백유도적T세포반응강도재무증상기명현고우애자병기.결론 HIV-1B형병독불동기인편마단백격발T세포분비IFN-γ반응소동,기중HIV-1 Gag단백특이성T세포재공제병정진전방면발휘료중요작용.
Objective To discuss the influence of HIV-1 specific T cell on disease progression of HIV-1 infection.Methods Thirty-seven patients with HIV-1 infection were recruited from a cohort study and their HIV-1 specific T cell responses were analyzed using overlapping peptides and ELISPOT assay.Resuits 83.78% (31/37) of candidates with HIV-1 infection responded to at least one peptide (magnitude was above 50 SFU/106 PBMCs).Peptides from HIV-1 subtype B proteins could elicit specific T cell responses in HIV-1 infections,and amongst them,the responses to HIV-1 Gag were the highest,not only in the frequencies of recognition,hut also in the magnitude of response (F=17.969,P<0.001).In addition,HIV-1 patients with different progression of disease expressed different T cell responses.The magnitude of response elicited by HIV-1 Gag protein in the patients with clinical asymptomatic stage was much higher than that in the patients with AIDS stage,although the responses to whole genome peptides between these two groups were similar.Conclusion The specific T cell responses elicited by different HIV-1 proteins are different,and the responses to HIV-1 Gag protein may play the most important role in the progression of HIV-1 infection.
