中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
11期
1000-1004
,共5页
张鹭%李国坚%吴继周%吴健林%陈茂伟%陈务卿%韦颖华%胡蝶飞%宁秋悦%庞裕
張鷺%李國堅%吳繼週%吳健林%陳茂偉%陳務卿%韋穎華%鬍蝶飛%寧鞦悅%龐裕
장로%리국견%오계주%오건림%진무위%진무경%위영화%호접비%저추열%방유
肝细胞癌%细胞因子%免疫状态%家族聚集
肝細胞癌%細胞因子%免疫狀態%傢族聚集
간세포암%세포인자%면역상태%가족취집
Hepatocellular carcinoma%Cytokines%Immune state%Familial aggregation
目的 探讨机体Th1/Th2细胞相关因子及免疫状态在肝癌发生及家族聚集性中的作用.方法 以IFN-γ和IL-2代表Th1类细胞因子,IL-4和IL-10代表Th2类细胞因子,利用酶联免疫吸附试验检测95对经配对设计的肝癌高发家族成员和无癌家族成员外周血IFN-γ、IL-2、IL-4、IL-10的水平.结果 肝癌高发家族成员中肝癌患者的Th1细胞因子水平显著高于无癌家族成员,Th2细胞因子水平显著低于无癌家族成员(P＜0.05)；肝癌高发家族中未患肝癌的家族成员,Th1/Th2细胞相关因子与无癌家族成员相比无明显差异(P＞0.05).结论 肝癌高发家族中的肝癌患者存在Th1/Th2平衡失调,向Th2方向漂移,抗乙型肝炎病毒(HBV)感染及抗肿瘤免疫功能低下导致HBV长期慢性感染,进而引起肝癌,在HBV感染家族聚集的基础上发生肝癌的家族聚集.肝癌高发家族中未患肝癌的成员Th1/Th2细胞因子水平无明显变化.推测肝癌的免疫功能异常是肝细胞癌变后逐渐形成的,而不是癌变前存在的免疫异常导致了癌变.
目的 探討機體Th1/Th2細胞相關因子及免疫狀態在肝癌髮生及傢族聚集性中的作用.方法 以IFN-γ和IL-2代錶Th1類細胞因子,IL-4和IL-10代錶Th2類細胞因子,利用酶聯免疫吸附試驗檢測95對經配對設計的肝癌高髮傢族成員和無癌傢族成員外週血IFN-γ、IL-2、IL-4、IL-10的水平.結果 肝癌高髮傢族成員中肝癌患者的Th1細胞因子水平顯著高于無癌傢族成員,Th2細胞因子水平顯著低于無癌傢族成員(P＜0.05)；肝癌高髮傢族中未患肝癌的傢族成員,Th1/Th2細胞相關因子與無癌傢族成員相比無明顯差異(P＞0.05).結論 肝癌高髮傢族中的肝癌患者存在Th1/Th2平衡失調,嚮Th2方嚮漂移,抗乙型肝炎病毒(HBV)感染及抗腫瘤免疫功能低下導緻HBV長期慢性感染,進而引起肝癌,在HBV感染傢族聚集的基礎上髮生肝癌的傢族聚集.肝癌高髮傢族中未患肝癌的成員Th1/Th2細胞因子水平無明顯變化.推測肝癌的免疫功能異常是肝細胞癌變後逐漸形成的,而不是癌變前存在的免疫異常導緻瞭癌變.
목적 탐토궤체Th1/Th2세포상관인자급면역상태재간암발생급가족취집성중적작용.방법 이IFN-γ화IL-2대표Th1류세포인자,IL-4화IL-10대표Th2류세포인자,이용매련면역흡부시험검측95대경배대설계적간암고발가족성원화무암가족성원외주혈IFN-γ、IL-2、IL-4、IL-10적수평.결과 간암고발가족성원중간암환자적Th1세포인자수평현저고우무암가족성원,Th2세포인자수평현저저우무암가족성원(P＜0.05)；간암고발가족중미환간암적가족성원,Th1/Th2세포상관인자여무암가족성원상비무명현차이(P＞0.05).결론 간암고발가족중적간암환자존재Th1/Th2평형실조,향Th2방향표이,항을형간염병독(HBV)감염급항종류면역공능저하도치HBV장기만성감염,진이인기간암,재HBV감염가족취집적기출상발생간암적가족취집.간암고발가족중미환간암적성원Th1/Th2세포인자수평무명현변화.추측간암적면역공능이상시간세포암변후축점형성적,이불시암변전존재적면역이상도치료암변.
Objective To investigate the effect of Th1/Th2 cytokines and immune state on the occurrence and familial aggregation of hepatocellular carcinoma(HCC).Methods Ninety-five members whose families have had two or even more HCC patients(high-occurrence families) were selected as the case group,by matching with the same nationality,gender,residential area,age±5 years old,95 members whose families had no any cancer were selected as the control.The level of peripheral blood Th1 type cytokines such as interferon-γ(IFN-γ),interleukin-2(IL-2) and Th2 type cytokines such as interleukin-4(IL-4),interleukin-10 (IL-10) were detected by enzyme linked immunosorbent assay(ELISA).Results There was a Th1/Th2 serum cytokine imbalance profile in members of HCC high-occurrence family.The levels of IFN-γ and IL-2 were significantly lower in members of HCC high-occurrence family than that of the controls.The levels of IL-4 and IL-10 were higher in members of HCC high-occurrence family than that of the controls.Conclusion There was a poor cellular immune state in members of HCC in the high-occurrence families.Th1 type cytokines was inhibited,and Th2 type cytokines was enhanced,so more susceptible to HBV chronic infection.It might be the mechanism of HCC occurrence and familial aggregation.
