CAJ | 학술논문

目的观察亚抑菌浓度(亚-MIC)苦参碱及与红霉素联用后对表皮葡萄球菌生物被膜的抑制作用以及对表皮葡萄球菌生物被膜形态学变化的影响.方法连续稀释法测定苦参碱和红霉素对表皮葡萄球菌的MIC；棋盘格法评价苦参碱和红霉素联用后对表皮葡萄球菌悬浮菌的抗菌活性；体外构建表皮葡萄球菌生物被膜,XTT减低法评价亚-MIC苦参碱及其与红霉素联用对表皮葡萄球菌生物被膜内细菌代谢及初始黏附能力的影响,扫描电镜观察用药后表皮葡萄球菌形态和生物被膜结构改变.结果红霉素对表皮葡萄球菌的MIC为7.8125 μg/ml,苦参碱对表皮葡萄球菌的MIC大于1000 μg/ml；两药联用时对表皮葡萄球菌悬浮菌有协同作用(FIC指数＜0.5).亚-MIC苦参碱对表皮葡萄球菌黏附作用无显著影响,与红霉素合用效果优于二者单独使用；亚-MIC苦参碱对表皮葡萄球菌生物被膜菌的代谢和形态均有抑制作用,其与红霉素联用时的作用弱于二者单独使用.结论亚-MIC苦参碱与红霉素对表皮葡萄球菌生物被膜的形成有显著影响；二者联合使用对表皮葡萄球菌悬浮菌及被膜菌黏附的抑制有协同作用,对表皮葡萄球菌生物被膜菌的代谢活性及形态影响均无协同作用.
목적 관찰아억균농도(아-MIC)고삼감급여홍매소련용후대표피포도구균생물피막적억제작용이급대표피포도구균생물피막형태학변화적영향.방법 련속희석법측정고삼감화홍매소대표피포도구균적MIC；기반격법평개고삼감화홍매소련용후대표피포도구균현부균적항균활성；체외구건표피포도구균생물피막,XTT감저법평개아-MIC고삼감급기여홍매소련용대표피포도구균생물피막내세균대사급초시점부능력적영향,소묘전경관찰용약후표피포도구균형태화생물피막결구개변.결과 홍매소대표피포도구균적MIC위7.8125 μg/ml,고삼감대표피포도구균적MIC대우1000 μg/ml；량약련용시대표피포도구균현부균유협동작용(FIC지수＜0.5).아-MIC고삼감대표피포도구균점부작용무현저영향,여홍매소합용효과우우이자단독사용；아-MIC고삼감대표피포도구균생물피막균적대사화형태균유억제작용,기여홍매소련용시적작용약우이자단독사용.결론 아-MIC고삼감여홍매소대표피포도구균생물피막적형성유현저영향；이자연합사용대표피포도구균현부균급피막균점부적억제유협동작용,대표피포도구균생물피막균적대사활성급형태영향균무협동작용.
Objective To observe the inhibitory effects of sub-MIC matrine alone and in combination with erythromycin on Staphylococcus epidermidis biofilms and their influences on morphological changes of the biofilms.Methods Minimum inhibitory concentrations (MIC) of matrine and erythromycin against Staphylococcus epidermidis were determined by the serial dilution method,antibacterial activity of matrine combined with erythromycin against planktonic S.epidermidis was evaluated by the checkerboard method.S.epidermidis biofilms were constructed in vitro,XTT reduction assay was used to evaluate influences of sub-MIC matrine alone and in combination with erythromycin on metabolism and adhesion of S.epidermidis biofilms,and scanning electronic microscope(SEM) was applied to observe the morphological and the structural changes of the biofilms.Results The MIC of erythromycin to S.epidermidis was 7.8125 μg/ml,while the MIC of matrine was greater than 1000 μg/ml,besides,a synergistic effect between erythronmycin and matrine on planktonic S.epidermidis was shown (FIC＜0.5).The sub-MIC matrine had no significant inhibitory effect on adhesion of S.epidermidis,and also the combination of the two agents was better than was used alone.However,the sub-MIC matrine had inhibitory effects on metabolism and morphology of S.epidermidis biofilms,and the combination of the two agents was weaker than was used alone.Conclusion Both the sub-MIC matrine and erythromycin had a significant inhibitory effect on S.epidermidis biofilm formation.Combination of the two agents showed synergistic effects on plankton and adhesion of S.epidermidis,but showed no synergistic effect on metabolism and morphology of the biofilms.